Detailed information
Bioinformatically predicted
Overview
| Name | pilA | Type | Machinery gene |
| Locus tag | GSU80_RS00535 | Genome accession | NZ_CP047231 |
| Coordinates | 122360..122800 (-) | Length | 146 a.a. |
| NCBI ID | WP_000360904.1 | Uniprot ID | P36647 |
| Organism | Escherichia coli strain BL21-AI | ||
| Function | type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake |
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Genomic Context
Location: 117360..127800
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GSU80_RS00515 (GSU80_00515) | coaE | 117850..118470 (-) | 621 | WP_001269520.1 | dephospho-CoA kinase | - |
| GSU80_RS21785 | - | 118495..118539 (+) | 45 | WP_120795372.1 | protein YacM | - |
| GSU80_RS00520 (GSU80_00520) | guaC | 118695..119738 (+) | 1044 | WP_001217335.1 | GMP reductase | - |
| GSU80_RS00525 (GSU80_00525) | hofC | 119773..120975 (-) | 1203 | WP_000157266.1 | protein transport protein HofC | - |
| GSU80_RS00530 (GSU80_00530) | pilB | 120965..122350 (-) | 1386 | WP_001025145.1 | type II secretion system protein GspE | Machinery gene |
| GSU80_RS00535 (GSU80_00535) | pilA | 122360..122800 (-) | 441 | WP_000360904.1 | prepilin peptidase-dependent pilin | Machinery gene |
| GSU80_RS00540 (GSU80_00540) | nadC | 123003..123896 (-) | 894 | WP_001135174.1 | carboxylating nicotinate-nucleotide diphosphorylase | - |
| GSU80_RS00545 (GSU80_00545) | ampD | 123984..124535 (+) | 552 | WP_000923721.1 | 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD | - |
| GSU80_RS00550 (GSU80_00550) | ampE | 124532..125386 (+) | 855 | WP_000172005.1 | beta-lactamase regulator AmpE | - |
| GSU80_RS00555 (GSU80_00555) | aroP | 125429..126802 (-) | 1374 | WP_000969915.1 | aromatic amino acid transporter AroP | - |
Sequence
Protein
Download Length: 146 a.a. Molecular weight: 15621.75 Da Isoelectric Point: 4.3938
>NTDB_id=358196 GSU80_RS00535 WP_000360904.1 122360..122800(-) (pilA) [Escherichia coli strain BL21-AI]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN
Nucleotide
Download Length: 441 bp
>NTDB_id=358196 GSU80_RS00535 WP_000360904.1 122360..122800(-) (pilA) [Escherichia coli strain BL21-AI]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilA | Haemophilus influenzae 86-028NP |
43.089 |
84.247 |
0.363 |