Detailed information    

insolico Bioinformatically predicted

Overview

Name   comO/pilO   Type   Machinery gene
Locus tag   GSF12_RS03030 Genome accession   NZ_CP047226
Coordinates   652992..653753 (+) Length   253 a.a.
NCBI ID   WP_201450424.1    Uniprot ID   -
Organism   Moraxella osloensis strain YV1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 647992..658753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF12_RS03010 (GSF12_03010) - 650240..650740 (-) 501 WP_159374277.1 IS630 family transposase -
  GSF12_RS03015 (GSF12_03015) - 650773..651153 (-) 381 WP_159374322.1 helix-turn-helix domain-containing protein -
  GSF12_RS03020 (GSF12_03020) pilM 651266..652318 (+) 1053 WP_159374323.1 pilus assembly protein PilM Machinery gene
  GSF12_RS03025 (GSF12_03025) pilN 652318..652989 (+) 672 WP_159374324.1 PilN domain-containing protein Machinery gene
  GSF12_RS03030 (GSF12_03030) comO/pilO 652992..653753 (+) 762 WP_201450424.1 type 4a pilus biogenesis protein PilO Machinery gene
  GSF12_RS03035 (GSF12_03035) comL/pilP 653740..654273 (+) 534 WP_159374325.1 pilus assembly protein PilP Machinery gene
  GSF12_RS03040 (GSF12_03040) comQ/pilQ 654291..656624 (+) 2334 WP_159374326.1 type IV pilus secretin family protein Machinery gene
  GSF12_RS03045 (GSF12_03045) - 656733..657269 (+) 537 WP_159374327.1 shikimate kinase -
  GSF12_RS03050 (GSF12_03050) aroB 657338..658435 (+) 1098 WP_159374328.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 253 a.a.        Molecular weight: 28746.01 Da        Isoelectric Point: 9.6337

>NTDB_id=358157 GSF12_RS03030 WP_201450424.1 652992..653753(+) (comO/pilO) [Moraxella osloensis strain YV1]
MKLTNPLKRPRLVKKPVQITAKKSFDVKAFAESFKSLDQNNYGSWPVPVKVTVLLFMVAVIAFLTWFLPISTKREEIAAA
EAEQQTLLDQYRQKESKARHLKEYQTQVLKMQSDFKELLNQLPKDTRISDLVDGINMVGVGSNIKFQDIKVEPEVSQEFF
IEQPIRIAALGDYHQFGSFISGLAKLPRIITLHDFEVANPSPSLDKLPETNLVLNVKTYRSKELTEEELKKAQKDNQNNP
AGSDNKEANNGNK

Nucleotide


Download         Length: 762 bp        

>NTDB_id=358157 GSF12_RS03030 WP_201450424.1 652992..653753(+) (comO/pilO) [Moraxella osloensis strain YV1]
GTGAAATTAACCAATCCGCTAAAAAGACCGAGACTCGTCAAAAAACCTGTTCAAATTACCGCAAAAAAATCGTTTGATGT
TAAAGCCTTCGCCGAGAGCTTTAAATCACTAGACCAAAACAATTATGGTAGCTGGCCTGTGCCTGTTAAGGTTACCGTCT
TGCTATTTATGGTCGCTGTTATCGCCTTTTTAACTTGGTTTTTACCGATTAGTACCAAACGTGAGGAAATTGCCGCTGCG
GAAGCCGAGCAGCAAACCTTGCTCGATCAATATCGCCAAAAAGAATCCAAAGCACGTCATTTAAAAGAATATCAAACGCA
AGTATTAAAAATGCAGTCTGATTTTAAAGAACTGCTCAATCAATTACCCAAAGACACCCGTATTTCTGACCTAGTGGATG
GGATTAATATGGTTGGTGTGGGCAGTAATATTAAATTTCAGGATATCAAAGTTGAACCTGAAGTGTCCCAAGAGTTCTTT
ATTGAGCAGCCAATTCGTATTGCCGCTTTAGGAGATTATCACCAATTTGGGTCATTCATTAGTGGTCTTGCTAAATTACC
TCGCATTATTACCCTGCATGACTTTGAAGTTGCCAACCCTAGTCCTAGCCTAGACAAGCTACCAGAAACCAATCTTGTTT
TGAACGTTAAAACTTATCGTTCAAAAGAGTTAACTGAAGAAGAATTGAAAAAGGCACAAAAAGATAATCAAAACAATCCA
GCTGGGTCTGACAATAAGGAAGCAAATAATGGCAATAAATAA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(78-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comO/pilO Acinetobacter baylyi ADP1

45.249

87.352

0.395
  pilO Acinetobacter baumannii D1279779

47.343

81.818

0.387

Multiple sequence alignment