Detailed information NTG0358150

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EA743_RS15190
• Genome accession: NZ_CP039341
• Coordinates: 3134185..3135303 (+)
• Length: 372 a.a.
• NCBI ID: WP_024432390.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain TG31986
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3129185..3140303

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EA743_RS15180 (EA743_015180)	-	3132301..3132993 (-)	693	WP_024432389.1	YggS family pyridoxal phosphate-dependent enzyme	-
EA743_RS15185 (EA743_015185)	pilT	3133120..3134157 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
EA743_RS15190 (EA743_015190)	pilU	3134185..3135303 (+)	1119	WP_024432390.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EA743_RS15195 (EA743_015195)	fur	3135373..3135810 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
EA743_RS15200 (EA743_015200)	-	3135922..3136320 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
EA743_RS15205 (EA743_015205)	-	3136375..3136698 (-)	324	WP_000442585.1	RnfH family protein	-
EA743_RS15210 (EA743_015210)	-	3136698..3137765 (-)	1068	WP_029776981.1	membrane protein	-
EA743_RS15220 (EA743_015220)	-	3137918..3138373 (-)	456	WP_000782976.1	bacteriohemerythrin	-
EA743_RS15225 (EA743_015225)	-	3138622..3139470 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41889.11 Da
• Isoelectric Point: 6.6945

>NTDB_id=358150 EA743_RS15190 WP_024432390.1 3134185..3135303(+) (pilU) [Acinetobacter baumannii strain TG31986]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDFAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=358150 EA743_RS15190 WP_024432390.1 3134185..3135303(+) (pilU) [Acinetobacter baumannii strain TG31986]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
TCGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392