Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E5677_RS11910 Genome accession   NZ_CP039251
Coordinates   2879629..2880069 (-) Length   146 a.a.
NCBI ID   WP_062843708.1    Uniprot ID   -
Organism   Psychrobacter sp. PAMC27889     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2869646..2879524 2879629..2880069 flank 105


Gene organization within MGE regions


Location: 2869646..2880069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5677_RS11860 (E5677_11860) - 2869646..2870401 (-) 756 WP_062843716.1 glycosyltransferase family 2 protein -
  E5677_RS11865 (E5677_11865) - 2870379..2871434 (-) 1056 WP_062843715.1 glycosyltransferase family 4 protein -
  E5677_RS11870 (E5677_11870) - 2871435..2872469 (-) 1035 WP_062843714.1 glycosyltransferase -
  E5677_RS11875 (E5677_11875) - 2872481..2873251 (-) 771 WP_062843713.1 glycosyltransferase family 25 protein -
  E5677_RS11880 (E5677_11880) - 2873284..2874033 (-) 750 WP_062843712.1 glycosyltransferase family 32 protein -
  E5677_RS11885 (E5677_11885) - 2874083..2874781 (-) 699 WP_062843711.1 FkbM family methyltransferase -
  E5677_RS11890 (E5677_11890) - 2874802..2876118 (-) 1317 WP_084387217.1 flippase -
  E5677_RS11895 (E5677_11895) - 2876193..2877461 (-) 1269 WP_062843710.1 sugar transferase -
  E5677_RS11900 (E5677_11900) - 2877613..2878032 (-) 420 WP_167541793.1 pilin -
  E5677_RS11905 (E5677_11905) - 2878205..2879524 (-) 1320 WP_228139886.1 O-antigen ligase family protein -
  E5677_RS11910 (E5677_11910) pilA 2879629..2880069 (-) 441 WP_062843708.1 pilin Machinery gene

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14797.88 Da        Isoelectric Point: 7.7782

>NTDB_id=357621 E5677_RS11910 WP_062843708.1 2879629..2880069(-) (pilA) [Psychrobacter sp. PAMC27889]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIAKSQASEAFTLIDGAKAEVNTNLEGNSCTNATAAKNTIAGKYGSLV
IAGTAASDASPTASTGCTLTYTFKGTGVSSQLASKVIGATLLNNGTLTKNATTTTVDADILPKSFT

Nucleotide


Download         Length: 441 bp        

>NTDB_id=357621 E5677_RS11910 WP_062843708.1 2879629..2880069(-) (pilA) [Psychrobacter sp. PAMC27889]
ATGAACGCTCAAAAAGGTTTTACCTTAATCGAACTAATGATCGTTGTTGCTATTATCGGTATTTTAGCTGCGATCGCTAT
TCCTGCTTATCAGAATTACATTGCTAAGTCACAAGCATCAGAAGCGTTTACTTTGATAGATGGAGCAAAAGCTGAGGTTA
ATACTAATTTAGAAGGCAATAGCTGTACTAATGCTACTGCTGCTAAAAATACAATTGCTGGTAAGTATGGTAGTTTAGTA
ATTGCTGGTACTGCTGCTAGTGATGCTTCGCCAACCGCATCAACGGGTTGTACACTTACTTATACATTTAAAGGAACTGG
TGTATCAAGTCAGCTAGCGTCTAAAGTAATTGGTGCTACATTACTTAACAACGGTACTTTGACTAAAAATGCTACCACTA
CTACAGTTGATGCTGATATCTTACCTAAATCATTCACTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter nosocomialis M2

58.389

100

0.596
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.667

100

0.438
  pilA Vibrio cholerae strain A1552

42.667

100

0.438
  pilA Vibrio cholerae C6706

42.667

100

0.438
  pilA Pseudomonas aeruginosa PAK

41.333

100

0.425

Multiple sequence alignment