Detailed information NTG0356786

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: E5A72_RS05200
• Genome accession: NZ_CP039025
• Coordinates: 1065447..1066505 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii ATCC 17978 substr. PMR-High
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1060447..1071505

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E5A72_RS05175 (E5A72_05175)	aroK	1060717..1061286 (-)	570	WP_000643712.1	shikimate kinase AroK	-
E5A72_RS05180 (E5A72_05180)	pilQ	1061298..1063463 (-)	2166	WP_001017031.1	type IV pilus secretin PilQ	Machinery gene
E5A72_RS05185 (E5A72_05185)	pilP	1063531..1064058 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
E5A72_RS05190 (E5A72_05190)	pilO	1064069..1064809 (-)	741	WP_000076102.1	type 4a pilus biogenesis protein PilO	Machinery gene
E5A72_RS05195 (E5A72_05195)	pilN	1064806..1065447 (-)	642	WP_000201223.1	PilN domain-containing protein	Machinery gene
E5A72_RS05200 (E5A72_05200)	pilM	1065447..1066505 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
E5A72_RS05205 (E5A72_05205)	ponA	1066668..1069223 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
E5A72_RS05210 (E5A72_05210)	-	1069241..1070065 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
E5A72_RS05215 (E5A72_05215)	-	1070068..1070400 (-)	333	WP_000169602.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=356786 E5A72_RS05200 WP_002014066.1 1065447..1066505(-) (pilM) [Acinetobacter baumannii ATCC 17978 substr. PMR-High]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=356786 E5A72_RS05200 WP_002014066.1 1065447..1066505(-) (pilM) [Acinetobacter baumannii ATCC 17978 substr. PMR-High]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGACGCGGTAGT
TCAGCAGGCGGCGCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCATTCTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423