Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   E5A72_RS05190 Genome accession   NZ_CP039025
Coordinates   1064069..1064809 (-) Length   246 a.a.
NCBI ID   WP_000076102.1    Uniprot ID   A0A1E3MBI9
Organism   Acinetobacter baumannii ATCC 17978 substr. PMR-High     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1059069..1069809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5A72_RS05170 (E5A72_05170) aroB 1059606..1060688 (-) 1083 WP_001193998.1 3-dehydroquinate synthase -
  E5A72_RS05175 (E5A72_05175) aroK 1060717..1061286 (-) 570 WP_000643712.1 shikimate kinase AroK -
  E5A72_RS05180 (E5A72_05180) pilQ 1061298..1063463 (-) 2166 WP_001017031.1 type IV pilus secretin PilQ Machinery gene
  E5A72_RS05185 (E5A72_05185) pilP 1063531..1064058 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  E5A72_RS05190 (E5A72_05190) pilO 1064069..1064809 (-) 741 WP_000076102.1 type 4a pilus biogenesis protein PilO Machinery gene
  E5A72_RS05195 (E5A72_05195) pilN 1064806..1065447 (-) 642 WP_000201223.1 PilN domain-containing protein Machinery gene
  E5A72_RS05200 (E5A72_05200) pilM 1065447..1066505 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  E5A72_RS05205 (E5A72_05205) ponA 1066668..1069223 (+) 2556 WP_000736677.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27849.61 Da        Isoelectric Point: 4.8357

>NTDB_id=356784 E5A72_RS05190 WP_000076102.1 1064069..1064809(-) (pilO) [Acinetobacter baumannii ATCC 17978 substr. PMR-High]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGTTDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=356784 E5A72_RS05190 WP_000076102.1 1064069..1064809(-) (pilO) [Acinetobacter baumannii ATCC 17978 substr. PMR-High]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTATGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
TATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGACAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCCTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E3MBI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.593

100

0.996
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment