Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VEA_RS05020 Genome accession   NC_013456
Coordinates   1033747..1034220 (-) Length   157 a.a.
NCBI ID   WP_012841419.1    Uniprot ID   -
Organism   Vibrio antiquarius     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1028747..1039220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VEA_RS05000 (VEA_002542) coaE 1029260..1029874 (-) 615 WP_012841417.1 dephospho-CoA kinase -
  VEA_RS05005 (VEA_002543) pilD 1029875..1030744 (-) 870 WP_012841418.1 A24 family peptidase Machinery gene
  VEA_RS05010 (VEA_002544) pilC 1030809..1032032 (-) 1224 WP_006743173.1 type II secretion system F family protein Machinery gene
  VEA_RS05015 (VEA_002545) pilB 1032056..1033741 (-) 1686 WP_006743172.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VEA_RS05020 (VEA_002546) pilA 1033747..1034220 (-) 474 WP_012841419.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VEA_RS05025 (VEA_002547) nadC 1034482..1035369 (-) 888 WP_012841420.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VEA_RS05030 (VEA_002548) ampD 1035462..1036013 (+) 552 WP_012841421.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VEA_RS05035 (VEA_002549) pdhR 1036419..1037186 (+) 768 WP_005396680.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16575.17 Da        Isoelectric Point: 8.4643

>NTDB_id=35643 VEA_RS05020 WP_012841419.1 1033747..1034220(-) (pilA) [Vibrio antiquarius]
MKNSKQKKQQGFTLIELMIVVAIIGILSAFAVPAYQNYTKKATLSEFPKAAAAAKLAVELCAHENASNETTFISECVKEE
NGIPAKFVLNNIEISVVKATDASVSGAIAVIAAADKVKGPIAKGEQYIMAASYKTQGLEWGASCKAGGKAQTDYCPN

Nucleotide


Download         Length: 474 bp        

>NTDB_id=35643 VEA_RS05020 WP_012841419.1 1033747..1034220(-) (pilA) [Vibrio antiquarius]
ATGAAAAACAGTAAACAAAAAAAACAACAGGGTTTTACTCTGATTGAATTAATGATTGTGGTGGCGATTATTGGCATCTT
ATCTGCTTTCGCAGTTCCTGCTTACCAAAATTATACTAAGAAAGCGACATTGTCAGAATTTCCAAAAGCAGCTGCGGCAG
CCAAATTAGCAGTAGAATTATGTGCCCATGAGAATGCCTCAAATGAAACAACATTTATTAGTGAATGTGTAAAGGAAGAA
AATGGAATTCCCGCTAAATTCGTTTTGAATAATATAGAAATTAGCGTTGTTAAAGCCACAGATGCTAGCGTTTCTGGTGC
GATAGCAGTTATTGCCGCAGCTGATAAAGTTAAGGGACCTATTGCTAAAGGCGAGCAATATATCATGGCTGCATCATATA
AAACTCAAGGCTTAGAGTGGGGGGCTTCATGTAAAGCTGGCGGTAAAGCACAAACAGACTACTGCCCAAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

57.325

100

0.573
  pilA Haemophilus influenzae Rd KW20

43.056

91.72

0.395
  pilA Haemophilus influenzae 86-028NP

41.781

92.994

0.389

Multiple sequence alignment