Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E5134_RS03440 Genome accession   NZ_CP038872
Coordinates   737880..739265 (+) Length   461 a.a.
NCBI ID   WP_061406214.1    Uniprot ID   -
Organism   Pasteurella multocida strain FCf71     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 732880..744265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5134_RS03410 (E5134_03410) - 733071..733922 (-) 852 WP_005722413.1 thymidylate synthase -
  E5134_RS03415 (E5134_03415) lgt 733919..734731 (-) 813 WP_010906471.1 prolipoprotein diacylglyceryl transferase -
  E5134_RS03420 (E5134_03420) - 734741..735535 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  E5134_RS03425 (E5134_03425) rppH 735538..736134 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  E5134_RS03430 (E5134_03430) ampD 736720..737286 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E5134_RS03435 (E5134_03435) pilA 737446..737880 (+) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  E5134_RS03440 (E5134_03440) pilB 737880..739265 (+) 1386 WP_061406214.1 GspE/PulE family protein Machinery gene
  E5134_RS03445 (E5134_03445) - 739265..740476 (+) 1212 WP_005722560.1 type II secretion system F family protein -
  E5134_RS03450 (E5134_03450) - 740476..741198 (+) 723 WP_005722562.1 A24 family peptidase -
  E5134_RS03455 (E5134_03455) coaE 741213..741833 (+) 621 WP_005722564.1 dephospho-CoA kinase -
  E5134_RS03460 (E5134_03460) yacG 741844..742047 (+) 204 WP_005722566.1 DNA gyrase inhibitor YacG -
  E5134_RS03465 (E5134_03465) - 742047..742316 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  E5134_RS03470 (E5134_03470) - 742765..744138 (+) 1374 WP_005722571.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52120.80 Da        Isoelectric Point: 6.3686

>NTDB_id=356118 E5134_RS03440 WP_061406214.1 737880..739265(+) (pilB) [Pasteurella multocida strain FCf71]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIVLTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRCCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=356118 E5134_RS03440 WP_061406214.1 737880..739265(+) (pilB) [Pasteurella multocida strain FCf71]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGTTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGTGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACAAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTTGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.811

98.482

0.579
  pilB Acinetobacter baumannii D1279779

39.757

100

0.425
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.412
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio cholerae strain A1552

38.272

100

0.403
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395

Multiple sequence alignment