Detailed information NTG0035553

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: BAMTA208_RS12200
• Genome accession: NC_017188
• Coordinates: 2424298..2424798 (-)
• Length: 166 a.a.
• NCBI ID: WP_013352868.1
• Uniprot ID: A0A9P1JIL5
• Organism: Bacillus amyloliquefaciens TA208
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2419298..2429798

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAMTA208_RS12155 (BAMTA208_12640)	-	2419417..2420211 (+)	795	WP_013352859.1	YqhG family protein	-
BAMTA208_RS12160 (BAMTA208_12645)	sinI	2420391..2420564 (+)	174	WP_013352860.1	anti-repressor SinI	Regulator
BAMTA208_RS12165 (BAMTA208_12650)	sinR	2420598..2420933 (+)	336	WP_014470659.1	transcriptional regulator SinR	Regulator
BAMTA208_RS12170 (BAMTA208_12655)	tasA	2420981..2421766 (-)	786	WP_013352862.1	biofilm matrix protein TasA	-
BAMTA208_RS12175 (BAMTA208_12660)	sipW	2421831..2422415 (-)	585	WP_013352863.1	signal peptidase I SipW	-
BAMTA208_RS12180 (BAMTA208_12665)	tapA	2422387..2423058 (-)	672	WP_013352864.1	amyloid fiber anchoring/assembly protein TapA	-
BAMTA208_RS12185 (BAMTA208_12670)	-	2423316..2423645 (+)	330	WP_013352865.1	DUF3889 domain-containing protein	-
BAMTA208_RS12190 (BAMTA208_12675)	-	2423686..2423865 (-)	180	WP_013352866.1	YqzE family protein	-
BAMTA208_RS12195 (BAMTA208_12680)	comGG	2423919..2424296 (-)	378	WP_013352867.1	competence type IV pilus minor pilin ComGG	Machinery gene
BAMTA208_RS12200 (BAMTA208_12685)	comGF	2424298..2424798 (-)	501	WP_013352868.1	competence type IV pilus minor pilin ComGF	Machinery gene
BAMTA208_RS12205 (BAMTA208_12690)	comGE	2424707..2425021 (-)	315	WP_014470662.1	competence type IV pilus minor pilin ComGE	Machinery gene
BAMTA208_RS12210 (BAMTA208_12695)	comGD	2425005..2425442 (-)	438	WP_013352869.1	competence type IV pilus minor pilin ComGD	Machinery gene
BAMTA208_RS12215 (BAMTA208_12700)	comGC	2425432..2425698 (-)	267	WP_044051905.1	competence type IV pilus major pilin ComGC	Machinery gene
BAMTA208_RS12220 (BAMTA208_12705)	comGB	2425745..2426782 (-)	1038	WP_013352871.1	competence type IV pilus assembly protein ComGB	Machinery gene
BAMTA208_RS12225 (BAMTA208_12710)	comGA	2426769..2427839 (-)	1071	WP_013352872.1	competence type IV pilus ATPase ComGA	Machinery gene
BAMTA208_RS12230 (BAMTA208_12715)	-	2428033..2428983 (-)	951	WP_013352873.1	magnesium transporter CorA family protein	-

Sequence

Protein

• Length: 166 a.a.
• Molecular weight: 17813.77 Da
• Isoelectric Point: 10.2182

>NTDB_id=35553 BAMTA208_RS12200 WP_013352868.1 2424298..2424798(-) (comGF) [Bacillus amyloliquefaciens TA208]
MVIITKSVQLSAAKKKCASAFSKQTGFTLLNVLVSLAVCLFLSGTLSPILHLFLSGRTDNGGIDSREHHIAVQQIMNECK
QAETVEPADGGRALTCRKTGGEEVRFEIYHTMIRKRVNGKGHVPILHNAASLTADVKNGLILVGITSVTGKKKQTAIPVY
SSLKGD

Nucleotide

• Length: 501 bp

>NTDB_id=35553 BAMTA208_RS12200 WP_013352868.1 2424298..2424798(-) (comGF) [Bacillus amyloliquefaciens TA208]
ATGGTGATTATTACAAAGTCTGTGCAGCTGTCCGCGGCGAAAAAGAAATGTGCCTCAGCATTCTCAAAACAGACTGGCTT
TACGCTTCTTAATGTTTTGGTCTCCTTGGCCGTCTGTCTCTTCTTATCAGGCACTCTGAGCCCGATTTTACATTTGTTTT
TATCAGGCCGGACCGATAACGGAGGAATAGACAGCCGGGAGCATCATATTGCCGTTCAGCAGATCATGAATGAATGTAAA
CAGGCTGAGACTGTGGAGCCTGCAGATGGCGGACGGGCGTTAACCTGCAGGAAAACAGGAGGCGAAGAAGTGCGTTTTGA
AATCTATCACACGATGATAAGGAAAAGAGTGAACGGAAAAGGCCATGTGCCGATCCTGCATAACGCTGCTTCATTAACGG
CTGATGTGAAAAACGGCCTGATTTTAGTGGGAATCACAAGCGTTACAGGTAAAAAGAAGCAGACGGCCATTCCGGTTTAC
AGCTCTTTAAAAGGTGATTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (65-152)

3D structure

• AlphaFold DB: A0A9P1JIL5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Bacillus subtilis subsp. subtilis str. 168	47.619	75.904	0.361