Detailed information NTG0354855

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: E4664_RS12555
• Genome accession: NZ_CP038500
• Coordinates: 2554092..2555210 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347035.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain CIAT758
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2549092..2560210

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E4664_RS12545 (E4664_12545)	-	2552208..2552900 (-)	693	WP_001108510.1	YggS family pyridoxal phosphate-dependent enzyme	-
E4664_RS12550 (E4664_12550)	pilT	2553027..2554064 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
E4664_RS12555 (E4664_12555)	pilU	2554092..2555210 (+)	1119	WP_000347035.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
E4664_RS12560 (E4664_12560)	fur	2555280..2555717 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
E4664_RS12565 (E4664_12565)	-	2555829..2556227 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
E4664_RS12570 (E4664_12570)	-	2556282..2556605 (-)	324	WP_000442585.1	RnfH family protein	-
E4664_RS12575 (E4664_12575)	-	2556605..2557672 (-)	1068	WP_005120426.1	hypothetical protein	-
E4664_RS12585 (E4664_12585)	-	2557825..2558280 (-)	456	WP_034129532.1	bacteriohemerythrin	-
E4664_RS12590 (E4664_12590)	-	2558533..2559381 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41821.98 Da
• Isoelectric Point: 6.5675

>NTDB_id=354855 E4664_RS12555 WP_000347035.1 2554092..2555210(+) (pilU) [Acinetobacter baumannii strain CIAT758]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDSQ

Nucleotide

• Length: 1119 bp

>NTDB_id=354855 E4664_RS12555 WP_000347035.1 2554092..2555210(+) (pilU) [Acinetobacter baumannii strain CIAT758]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACAGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.817	100	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39