Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   E4J95_RS07590 Genome accession   NZ_CP038229
Coordinates   1586121..1586618 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain MJ163     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1581121..1591618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4J95_RS07565 (E4J95_07920) gcvPB 1581283..1582755 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  E4J95_RS07570 (E4J95_07925) gcvPA 1582748..1584094 (-) 1347 WP_045177127.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E4J95_RS07575 (E4J95_07930) gcvT 1584114..1585205 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  E4J95_RS07580 (E4J95_07935) - 1585364..1585888 (-) 525 WP_001015121.1 shikimate kinase -
  E4J95_RS07585 (E4J95_07940) - 1585878..1586024 (-) 147 WP_001789879.1 hypothetical protein -
  E4J95_RS07590 (E4J95_07945) comGF 1586121..1586618 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  E4J95_RS07595 (E4J95_07950) comGE 1586536..1586835 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E4J95_RS07600 (E4J95_07955) comGD 1586822..1587268 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  E4J95_RS07605 (E4J95_07960) comGC 1587246..1587557 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E4J95_RS07610 (E4J95_07965) comGB 1587571..1588641 (-) 1071 WP_045176802.1 competence type IV pilus assembly protein ComGB Machinery gene
  E4J95_RS07615 (E4J95_07970) comGA 1588613..1589587 (-) 975 WP_000697227.1 competence type IV pilus ATPase ComGA Machinery gene
  E4J95_RS07620 (E4J95_07975) - 1589639..1590262 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E4J95_RS07625 (E4J95_07980) - 1590259..1590588 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E4J95_RS07630 (E4J95_07985) - 1590588..1591574 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=352439 E4J95_RS07590 WP_001789864.1 1586121..1586618(-) (comGF) [Staphylococcus aureus strain MJ163]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=352439 E4J95_RS07590 WP_001789864.1 1586121..1586618(-) (comGF) [Staphylococcus aureus strain MJ163]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment