Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   E4J95_RS07615 Genome accession   NZ_CP038229
Coordinates   1588613..1589587 (-) Length   324 a.a.
NCBI ID   WP_000697227.1    Uniprot ID   -
Organism   Staphylococcus aureus strain MJ163     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1583613..1594587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4J95_RS07575 (E4J95_07930) gcvT 1584114..1585205 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  E4J95_RS07580 (E4J95_07935) - 1585364..1585888 (-) 525 WP_001015121.1 shikimate kinase -
  E4J95_RS07585 (E4J95_07940) - 1585878..1586024 (-) 147 WP_001789879.1 hypothetical protein -
  E4J95_RS07590 (E4J95_07945) comGF 1586121..1586618 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  E4J95_RS07595 (E4J95_07950) comGE 1586536..1586835 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E4J95_RS07600 (E4J95_07955) comGD 1586822..1587268 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  E4J95_RS07605 (E4J95_07960) comGC 1587246..1587557 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E4J95_RS07610 (E4J95_07965) comGB 1587571..1588641 (-) 1071 WP_045176802.1 competence type IV pilus assembly protein ComGB Machinery gene
  E4J95_RS07615 (E4J95_07970) comGA 1588613..1589587 (-) 975 WP_000697227.1 competence type IV pilus ATPase ComGA Machinery gene
  E4J95_RS07620 (E4J95_07975) - 1589639..1590262 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E4J95_RS07625 (E4J95_07980) - 1590259..1590588 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E4J95_RS07630 (E4J95_07985) - 1590588..1591574 (-) 987 WP_000161314.1 ROK family glucokinase -
  E4J95_RS07635 (E4J95_07990) - 1591571..1591774 (-) 204 WP_000087561.1 YqgQ family protein -
  E4J95_RS07640 (E4J95_07995) - 1591755..1593218 (-) 1464 WP_169449281.1 rhomboid family intramembrane serine protease -
  E4J95_RS07645 (E4J95_08000) - 1593239..1593769 (-) 531 WP_072384884.1 5-formyltetrahydrofolate cyclo-ligase -
  E4J95_RS07650 (E4J95_08005) rpmG 1593978..1594127 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36842.96 Da        Isoelectric Point: 8.8508

>NTDB_id=352444 E4J95_RS07615 WP_000697227.1 1588613..1589587(-) (comGA) [Staphylococcus aureus strain MJ163]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=352444 E4J95_RS07615 WP_000697227.1 1588613..1589587(-) (comGA) [Staphylococcus aureus strain MJ163]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGGTTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.383

100

0.994
  comGA Staphylococcus aureus N315

99.383

100

0.994

Multiple sequence alignment