Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   E3220_RS12700 Genome accession   NZ_CP038208
Coordinates   2352236..2353741 (-) Length   501 a.a.
NCBI ID   WP_016209723.1    Uniprot ID   A0A9Q5VFW7
Organism   Piscirickettsia salmonis EM-90     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2307521..2369563 2352236..2353741 within 0


Gene organization within MGE regions


Location: 2307521..2369563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3220_RS12505 (E3220_11975) - 2307521..2308495 (-) 975 WP_054300271.1 IS30 family transposase -
  E3220_RS12510 (E3220_11980) - 2308571..2309590 (-) 1020 WP_054300164.1 transposase -
  E3220_RS12515 - 2309638..2309784 (-) 147 WP_155047000.1 hypothetical protein -
  E3220_RS12520 (E3220_11985) - 2309989..2310174 (-) 186 WP_155047001.1 hypothetical protein -
  E3220_RS12525 (E3220_11990) - 2311073..2312142 (+) 1070 Protein_2451 IS481 family transposase -
  E3220_RS12530 (E3220_11995) - 2312190..2313251 (-) 1062 WP_075274966.1 transposase -
  E3220_RS12535 (E3220_12000) - 2313332..2313640 (-) 309 WP_016211201.1 hypothetical protein -
  E3220_RS12540 (E3220_12005) - 2313755..2315071 (-) 1317 WP_016211206.1 MFS transporter -
  E3220_RS12545 (E3220_12010) - 2315533..2316750 (-) 1218 WP_016211208.1 multidrug effflux MFS transporter -
  E3220_RS12550 (E3220_12015) - 2316892..2317788 (+) 897 WP_016211205.1 LysR family transcriptional regulator -
  E3220_RS12555 (E3220_12020) - 2317875..2318873 (-) 999 WP_016211203.1 ATP-binding protein -
  E3220_RS12560 (E3220_12025) aroK 2318982..2319506 (-) 525 WP_032126430.1 shikimate kinase AroK -
  E3220_RS12565 (E3220_12030) - 2319754..2321013 (-) 1260 WP_016211204.1 type IV pilus secretin PilQ -
  E3220_RS12570 - 2321259..2321402 (+) 144 WP_016211207.1 hypothetical protein -
  E3220_RS12575 (E3220_12035) - 2321540..2322013 (-) 474 WP_016212222.1 hypothetical protein -
  E3220_RS12580 (E3220_12040) - 2322010..2322525 (-) 516 WP_016212225.1 PilN domain-containing protein -
  E3220_RS12585 (E3220_12045) - 2322522..2323334 (-) 813 WP_016212223.1 hypothetical protein -
  E3220_RS12590 (E3220_12050) - 2323335..2323910 (-) 576 WP_075273327.1 IS630 family transposase -
  E3220_RS12595 (E3220_12055) - 2323856..2324221 (-) 366 WP_032126362.1 IS630 transposase-related protein -
  E3220_RS12600 (E3220_12060) - 2324486..2326816 (+) 2331 WP_016211610.1 penicillin-binding protein 1A -
  E3220_RS12605 (E3220_12065) - 2326937..2328952 (+) 2016 WP_129556603.1 methyltransferase domain-containing protein -
  E3220_RS12610 (E3220_12070) - 2329136..2332528 (+) 3393 WP_075274967.1 hypothetical protein -
  E3220_RS12615 (E3220_12075) - 2332593..2332898 (+) 306 WP_016212386.1 hypothetical protein -
  E3220_RS12620 (E3220_12080) - 2333068..2334168 (+) 1101 WP_075274968.1 transposase -
  E3220_RS12625 (E3220_12085) - 2334416..2335672 (+) 1257 WP_155049897.1 hypothetical protein -
  E3220_RS12630 (E3220_12090) - 2335855..2336184 (-) 330 WP_016212095.1 phospholipase D-like domain-containing protein -
  E3220_RS12635 (E3220_12095) asnS 2336611..2338008 (-) 1398 WP_016209721.1 asparagine--tRNA ligase -
  E3220_RS12640 (E3220_12100) trbB 2338128..2339075 (-) 948 WP_051307313.1 P-type conjugative transfer ATPase TrbB -
  E3220_RS12645 (E3220_12105) - 2339072..2339587 (-) 516 WP_032126703.1 S26 family signal peptidase -
  E3220_RS12650 - 2340090..2340773 (-) 684 Protein_2476 type IV secretion system protein -
  E3220_RS12655 (E3220_12115) - 2340770..2341078 (-) 309 WP_230383332.1 hypothetical protein -
  E3220_RS12660 (E3220_12120) - 2341095..2342324 (-) 1230 WP_016209703.1 TrbI/VirB10 family protein -
  E3220_RS12665 (E3220_12125) - 2342324..2343367 (-) 1044 WP_016209722.1 TrbG/VirB9 family P-type conjugative transfer protein -
  E3220_RS12670 (E3220_12130) trbF 2343367..2344050 (-) 684 WP_129556606.1 conjugal transfer protein TrbF -
  E3220_RS12675 (E3220_12135) trbE 2344047..2346536 (-) 2490 WP_016209720.1 conjugal transfer protein TrbE -
  E3220_RS12680 (E3220_12140) - 2346553..2346807 (-) 255 WP_017377396.1 VirB3 family type IV secretion system protein -
  E3220_RS12685 (E3220_12145) - 2346808..2347101 (-) 294 WP_051307316.1 TrbC/VirB2 family protein -
  E3220_RS12690 (E3220_12150) - 2347950..2349107 (+) 1158 WP_032126705.1 efflux RND transporter periplasmic adaptor subunit -
  E3220_RS12695 (E3220_12155) - 2349127..2352234 (+) 3108 WP_016209727.1 efflux RND transporter permease subunit -
  E3220_RS12700 (E3220_12160) comM 2352236..2353741 (-) 1506 WP_016209723.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  E3220_RS12705 (E3220_12165) - 2353769..2354050 (-) 282 WP_016209714.1 ubiquinone biosynthesis accessory factor UbiK -
  E3220_RS12710 (E3220_12170) - 2354199..2354540 (+) 342 WP_016209709.1 P-II family nitrogen regulator -
  E3220_RS12715 (E3220_12175) speA 2354660..2356540 (+) 1881 WP_016209712.1 biosynthetic arginine decarboxylase -
  E3220_RS12720 (E3220_12180) - 2356625..2358223 (-) 1599 WP_059372650.1 hypothetical protein -
  E3220_RS12725 (E3220_12185) - 2358241..2359356 (-) 1116 WP_051307314.1 glycosyltransferase -
  E3220_RS12730 (E3220_12190) aroF 2359484..2360482 (-) 999 WP_016209706.1 3-deoxy-7-phosphoheptulonate synthase -
  E3220_RS12735 (E3220_12195) trpA 2360486..2361244 (-) 759 WP_016209700.1 tryptophan synthase subunit alpha -
  E3220_RS12740 (E3220_12200) trpB 2361246..2362445 (-) 1200 WP_016209702.1 tryptophan synthase subunit beta -
  E3220_RS12745 (E3220_12205) - 2362429..2363100 (-) 672 WP_016209711.1 phosphoribosylanthranilate isomerase -
  E3220_RS12750 (E3220_12210) - 2363122..2363898 (-) 777 WP_016209710.1 indole-3-glycerol-phosphate synthase -
  E3220_RS12755 (E3220_12215) trpD 2363902..2364876 (-) 975 WP_230391886.1 anthranilate phosphoribosyltransferase -
  E3220_RS12760 (E3220_12220) - 2364902..2365480 (-) 579 WP_016209697.1 aminodeoxychorismate/anthranilate synthase component II -
  E3220_RS12765 (E3220_12225) - 2365477..2366946 (-) 1470 WP_016209713.1 anthranilate synthase component I family protein -
  E3220_RS12770 (E3220_12230) trpR 2366990..2367277 (-) 288 WP_016209719.1 trp operon repressor -
  E3220_RS12775 (E3220_12235) - 2367478..2368074 (+) 597 WP_016209699.1 DMT family transporter -
  E3220_RS12780 (E3220_12240) - 2368380..2368754 (+) 375 WP_173018591.1 IS630 transposase-related protein -
  E3220_RS12785 - 2368811..2368966 (+) 156 WP_155046757.1 hypothetical protein -
  E3220_RS12790 (E3220_12250) - 2369111..2369563 (+) 453 WP_075274971.1 IS630 family transposase -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54520.47 Da        Isoelectric Point: 7.9377

>NTDB_id=352328 E3220_RS12700 WP_016209723.1 2352236..2353741(-) (comM) [Piscirickettsia salmonis EM-90]
MKLAIVYSRAQVAVAAPQVTVEVHISNGLPGFTIVGLPDAAVKESRERVRSAIQNSGFNFPQRRITVNLAPADLPKDGGR
FDLAIAIGILASSGQIDADILNGYEFIGELALSGEVRSVTGALTSAKAVKECQRKLIIADDNANLAALVEGLSVYRANNL
ASISAFLKGEGDLEQAHFQVDTGQTHYLDMADVKGQAHAKRALEIAAAGRHHLLFVGPPGTGKTMLASRLPGVLPALSNQ
EALESAAVHSLTSAEVDLSCWFIPKFASPHHTASSIAMVGGGSVPKPGEISRAHHGVLFLDELPEFDRKVLEVLREPLES
GQVDIIRASHRASFPASFQLIAAMNPCPCGHLGSQEQRCRCSQLQIKRYLNKLSGPLLERIDLQIEVPRLLQHELSSTQV
AESSVVIRQRVEVARQLQLKRQQCPNAGLMPRQIEVFCSLGQQESNLLTRAIEKFGLSARVYYRLLKVARTIADLEQSTD
IKLQHVQESLAYRSLDRRQSE

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=352328 E3220_RS12700 WP_016209723.1 2352236..2353741(-) (comM) [Piscirickettsia salmonis EM-90]
ATGAAATTAGCAATTGTTTACTCGCGTGCCCAGGTTGCGGTGGCTGCGCCACAAGTGACTGTTGAGGTGCATATTTCTAA
TGGCTTACCAGGCTTTACCATCGTAGGATTGCCGGATGCGGCGGTAAAAGAAAGCCGTGAGCGCGTGCGCAGCGCGATTC
AAAACTCCGGCTTTAATTTCCCACAACGGCGTATTACTGTGAACCTTGCGCCTGCAGACTTACCGAAAGATGGTGGGCGC
TTTGATTTAGCGATTGCCATCGGCATTTTGGCATCGTCGGGCCAAATTGATGCCGATATTTTAAACGGCTATGAATTTAT
TGGTGAACTTGCCCTCTCTGGTGAAGTACGTTCTGTCACTGGGGCCTTAACGAGTGCTAAAGCGGTTAAAGAATGTCAGC
GTAAATTAATCATCGCTGATGATAATGCCAATTTAGCGGCCTTAGTCGAGGGGTTATCTGTATATCGGGCGAATAATTTA
GCCAGCATTAGTGCTTTTTTAAAAGGTGAAGGTGATTTAGAACAGGCACATTTTCAAGTCGATACCGGGCAAACGCACTA
TTTAGATATGGCGGATGTCAAAGGCCAGGCGCATGCTAAGCGCGCTTTAGAAATTGCCGCCGCAGGTCGTCATCATTTAC
TTTTTGTCGGCCCTCCGGGAACAGGCAAAACCATGCTTGCCTCACGGTTACCTGGCGTTTTGCCAGCCTTATCCAATCAA
GAAGCACTAGAATCAGCAGCAGTGCATAGCTTAACGAGTGCTGAAGTTGATTTATCATGTTGGTTTATCCCTAAATTTGC
CAGCCCTCATCATACCGCATCGAGTATTGCCATGGTTGGGGGTGGCTCAGTGCCGAAACCGGGTGAAATTTCTCGGGCCC
ATCATGGGGTATTATTTTTAGATGAGTTGCCTGAATTTGATCGCAAAGTCTTAGAAGTATTACGAGAGCCATTAGAATCG
GGGCAGGTTGATATTATTCGCGCAAGTCACCGGGCCTCTTTTCCGGCTTCGTTTCAGCTTATTGCAGCAATGAACCCGTG
TCCCTGTGGCCATTTAGGTTCACAAGAGCAACGTTGTCGTTGCAGTCAGTTACAGATCAAGCGCTATTTAAATAAGCTCT
CTGGACCGTTACTTGAGCGTATTGACCTGCAAATTGAAGTACCACGGTTATTGCAACATGAACTAAGCAGTACACAAGTG
GCCGAGAGCAGTGTGGTGATACGGCAGCGGGTCGAGGTTGCACGTCAATTACAATTAAAACGTCAGCAGTGCCCGAATGC
TGGATTAATGCCTAGGCAAATTGAAGTGTTTTGTTCTCTGGGTCAACAAGAATCGAACTTGCTGACACGGGCAATAGAAA
AGTTTGGCTTATCTGCACGGGTGTATTATCGCTTGTTAAAGGTTGCACGGACCATTGCTGACCTTGAGCAAAGTACAGAC
ATTAAACTGCAGCATGTGCAAGAAAGCTTGGCCTATCGCAGCTTGGATCGACGTCAGAGTGAGTGA

Domains

Predicted by InterproScan.

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(402-493)

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Vibrio cholerae strain A1552

53.493

100

0.535
  comM Haemophilus influenzae Rd KW20

53.28

100

0.535
  comM Glaesserella parasuis strain SC1401

53.082

100

0.533
  comM Vibrio campbellii strain DS40M4

53.094

100

0.531
  comM Legionella pneumophila str. Paris

51.21

99.002

0.507
  comM Legionella pneumophila strain ERS1305867

51.21

99.002

0.507
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

46.654

100

0.473

Multiple sequence alignment