Detailed information NTG0351650

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: E3U44_RS04600
• Genome accession: NZ_CP038033
• Coordinates: 950069..951184 (+)
• Length: 371 a.a.
• NCBI ID: WP_134356880.1
• Uniprot ID: -
• Organism: Nitrosococcus wardiae strain D1FHS
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 945069..956184

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E3U44_RS04580 (E3U44_04580)	rpmE	945099..945305 (+)	207	WP_134356876.1	50S ribosomal protein L31	-
E3U44_RS04585 (E3U44_04585)	-	945459..946778 (+)	1320	WP_134356877.1	malic enzyme-like NAD(P)-binding protein	-
E3U44_RS04590 (E3U44_04590)	-	946768..947382 (-)	615	WP_134356878.1	hypothetical protein	-
E3U44_RS04595 (E3U44_04595)	-	947405..949843 (-)	2439	WP_134356879.1	penicillin-binding protein 1A	-
E3U44_RS04600 (E3U44_04600)	pilM	950069..951184 (+)	1116	WP_134356880.1	pilus assembly protein PilM	Machinery gene
E3U44_RS04605 (E3U44_04605)	-	951184..951753 (+)	570	WP_134356881.1	PilN domain-containing protein	-
E3U44_RS04610 (E3U44_04610)	-	951753..952370 (+)	618	WP_134356882.1	type 4a pilus biogenesis protein PilO	-
E3U44_RS04615 (E3U44_04615)	-	952367..952945 (+)	579	WP_134356883.1	pilus assembly protein PilP	-
E3U44_RS04620 (E3U44_04620)	pilQ	952962..955061 (+)	2100	WP_134356884.1	type IV pilus secretin PilQ	-
E3U44_RS04625 (E3U44_04625)	aroK	955230..955778 (+)	549	WP_134356885.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 40223.96 Da
• Isoelectric Point: 4.7506

>NTDB_id=351650 E3U44_RS04600 WP_134356880.1 950069..951184(+) (pilM) [Nitrosococcus wardiae strain D1FHS]
MLNSLDRISNSLDSGKKSSSLFSRKPPFLLGIDISSSAVKLLELSRKGERSCVENYAIEPLPPHTVVENKVEDVEALGEA
IKRAVKRSGTRIKKAAAAVAGSAVITKIISVPNTLSERELESQIELEAGQYIPYPLEEVNLDFEVLTPSEKDPEAVDVLL
AACRSENVEARVAALELAGLTPAVMDVEAYAMEKVFPLIASQMPSRGKGQTIAVVDVGATLTTINVLHDYKIIYTREQIF
GGNQLTEEIQRRYGLSYEEANLAKRQGGLPDNYIPEILEPFKKAIAQQVSRAFQFFFSSTQYNSVDHVVLAGGCASIPGV
DELIEHMAGTTVSIANPFADMALGSQVKAKELYSDAPALMTACGLALRSFD

Nucleotide

• Length: 1116 bp

>NTDB_id=351650 E3U44_RS04600 WP_134356880.1 950069..951184(+) (pilM) [Nitrosococcus wardiae strain D1FHS]
ATGCTAAATTCATTGGATCGTATTAGTAATTCACTTGACTCTGGGAAAAAATCATCGAGCTTATTTTCTCGCAAACCTCC
TTTTCTGTTGGGGATAGATATAAGCTCATCAGCCGTCAAGCTGCTTGAACTTAGCCGAAAGGGAGAACGCTCTTGTGTAG
AAAATTATGCGATTGAGCCTTTGCCACCTCATACTGTGGTTGAAAATAAGGTTGAAGATGTGGAAGCCCTTGGTGAGGCG
ATTAAAAGGGCGGTTAAACGTTCGGGAACCCGAATAAAAAAAGCGGCTGCAGCAGTCGCTGGTTCAGCAGTAATTACGAA
GATCATCTCCGTTCCAAATACCCTTTCAGAGCGCGAACTCGAGAGTCAGATCGAGTTAGAGGCAGGACAGTATATTCCTT
ACCCCCTGGAGGAAGTTAATCTGGATTTTGAGGTGCTGACTCCCTCTGAAAAAGATCCTGAAGCAGTGGATGTGCTGCTT
GCTGCTTGCCGCAGTGAAAACGTTGAAGCACGGGTAGCCGCATTGGAACTGGCAGGCTTAACACCCGCAGTAATGGATGT
AGAGGCTTATGCTATGGAAAAAGTTTTTCCTTTGATTGCGAGCCAGATGCCGAGCCGAGGGAAAGGGCAAACCATTGCTG
TTGTGGATGTAGGTGCAACTTTAACCACGATTAATGTGTTGCATGATTACAAAATTATTTATACCCGGGAACAAATATTT
GGTGGTAATCAGCTTACGGAAGAAATACAACGGCGATATGGCTTGTCCTATGAGGAAGCTAATTTAGCGAAGCGTCAAGG
GGGATTGCCAGATAATTATATTCCCGAGATCTTAGAACCTTTCAAAAAGGCAATTGCCCAGCAGGTCAGCCGTGCTTTTC
AATTCTTCTTTTCTTCAACCCAATATAACAGCGTTGATCATGTGGTATTGGCAGGTGGATGTGCTTCTATTCCTGGAGTA
GACGAACTGATTGAGCATATGGCGGGTACTACTGTTTCGATTGCGAATCCTTTTGCTGATATGGCGTTAGGCTCTCAGGT
TAAAGCTAAGGAGTTGTACAGTGATGCTCCAGCTTTGATGACTGCCTGCGGCTTAGCACTTAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (31-370)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.576	94.07	0.485
comM	Acinetobacter nosocomialis M2	51.576	94.07	0.485
comM	Acinetobacter baylyi ADP1	48.433	94.609	0.458
pilM	Legionella pneumophila strain ERS1305867	45.584	94.609	0.431