Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DDF84_RS15740 Genome accession   NZ_CP037900
Coordinates   3380082..3381803 (+) Length   573 a.a.
NCBI ID   WP_024570928.1    Uniprot ID   A0A2L0XA98
Organism   Cupriavidus metallidurans strain BS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3375082..3386803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDF84_RS15710 (DDF84_015710) proB 3375145..3376263 (-) 1119 WP_008647250.1 glutamate 5-kinase -
  DDF84_RS15715 (DDF84_015715) obgE 3376301..3377398 (-) 1098 WP_008647251.1 GTPase ObgE -
  DDF84_RS15720 (DDF84_015720) rpmA 3377524..3377784 (-) 261 WP_008647258.1 50S ribosomal protein L27 -
  DDF84_RS15725 (DDF84_015725) rplU 3377836..3378147 (-) 312 WP_008647259.1 50S ribosomal protein L21 -
  DDF84_RS15730 (DDF84_015730) ispB 3378657..3379586 (+) 930 WP_017514705.1 octaprenyl diphosphate synthase -
  DDF84_RS15740 (DDF84_015740) pilF 3380082..3381803 (+) 1722 WP_024570928.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DDF84_RS15745 (DDF84_015745) - 3381833..3383098 (+) 1266 WP_017515802.1 type II secretion system F family protein -
  DDF84_RS15750 (DDF84_015750) pilD 3383117..3383986 (+) 870 WP_024570929.1 prepilin peptidase Machinery gene
  DDF84_RS15755 (DDF84_015755) coaE 3384057..3384701 (+) 645 WP_017515849.1 dephospho-CoA kinase -
  DDF84_RS15760 (DDF84_015760) zapD 3384924..3385682 (+) 759 WP_008650332.1 cell division protein ZapD -
  DDF84_RS15765 (DDF84_015765) - 3385691..3385882 (+) 192 WP_008650330.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62938.57 Da        Isoelectric Point: 7.5068

>NTDB_id=350567 DDF84_RS15740 WP_024570928.1 3380082..3381803(+) (pilF) [Cupriavidus metallidurans strain BS1]
MTLGLALAQSRRIAPALLAQLEQSAREKKTQLIDEIIGSGTMSAHDVAVFAADKYQLPLLDLSEYNLSRVPSALSGNREF
HAHRLLPLGRRENRLIVAMSDPANQAGVDVIRQKFSLPVETVVVEHDKLMKHVRSAGEALGTLKNALPTPTAQKMIDYDP
VAAASARKVPGANDIDDAPVVRFLQKLLTEAFHRGASDLHFEPFEFFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVLS
RLDISEKRVPQDGRMKLLISMPKDKDGKETVDKAIDFRVSTLPTLFGEKIVMRILDSSSDKLDIDQLGYEPQQKALLQEV
IKRPYGMVLVTGPTGSGKTVSLYTFLNMLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDVIMV
GEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCTCKREGDLAPQVLID
AGFQESDLDGTWKPYHPVGCDRCSGSGYKGRCGIYQVMPITETIQQIILSHGTALQIAEQARKEGVLSLREAGLLKVKQG
VTSLEEVLATTNT

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=350567 DDF84_RS15740 WP_024570928.1 3380082..3381803(+) (pilF) [Cupriavidus metallidurans strain BS1]
ATGACACTAGGCCTCGCGCTTGCCCAGAGCCGGCGGATCGCGCCGGCTCTGCTTGCCCAGTTGGAGCAATCGGCCCGGGA
AAAGAAGACGCAGCTGATCGACGAGATCATTGGCAGCGGCACCATGTCCGCCCATGACGTCGCCGTGTTCGCCGCCGACA
AGTACCAGCTCCCGCTGCTCGACCTCTCCGAATACAACCTCAGCCGCGTGCCGTCGGCACTCTCGGGCAACCGCGAGTTC
CATGCGCACCGGCTCCTGCCGCTGGGCCGTCGGGAAAACCGCCTGATCGTGGCGATGTCCGATCCGGCCAACCAGGCCGG
CGTCGACGTGATCCGCCAGAAGTTCAGCCTACCCGTGGAAACGGTCGTCGTCGAACACGACAAGCTGATGAAGCATGTCC
GATCTGCCGGCGAGGCACTTGGCACGCTCAAGAACGCCCTGCCGACGCCCACGGCTCAGAAGATGATCGACTATGACCCG
GTGGCCGCCGCAAGCGCGCGCAAGGTCCCCGGCGCCAACGACATCGACGACGCGCCAGTGGTCCGCTTCCTGCAGAAGCT
GCTGACGGAAGCCTTCCATCGCGGCGCGTCGGACCTTCACTTCGAACCATTCGAGTTCTTCTACCGCATTCGCTTCCGCG
TGGACGGCGTGCTGCAGGAAGTCGCGCGGCCACCGCTCGATATCCGCGACAAGATCGCCACGCGTATCAAGGTGCTATCG
CGCCTGGACATCTCGGAAAAGCGCGTGCCGCAGGACGGCCGGATGAAGCTGCTGATCTCGATGCCGAAAGACAAGGACGG
CAAGGAGACCGTCGACAAGGCGATCGACTTCCGCGTCTCCACGCTGCCCACGTTGTTCGGCGAAAAGATCGTGATGCGGA
TTCTCGATTCGTCGAGCGACAAGCTCGACATCGACCAACTCGGCTATGAGCCGCAGCAGAAGGCACTGCTGCAGGAAGTG
ATCAAGCGGCCATACGGGATGGTGCTGGTGACTGGCCCGACCGGCAGCGGCAAGACTGTGTCGCTCTATACGTTCCTGAA
CATGCTGAACCAGGGCGACATTAATATCTCCACGGCCGAGGACCCGGCCGAAATCCAGTTGCCCGGCATCAACCAGGTCA
ACGTGAACGACAAGGCCGGCCTGACCTTCGCCGCCGCGCTGAAATCGTTCCTGCGCCAGGATCCCGACGTGATCATGGTC
GGCGAAATTCGTGACCTCGAGACGGCCGACATCTCCATCAAGGCCGCGCAGACCGGTCACCTGGTGCTGTCCACGCTGCA
CACGAACGACGCGCCGACCACGCTGACACGCCTGATGAATATGGGCGTGGCACCGTTCAACATCGCCTCGTCGGTCCTGC
TGATCACCGCGCAGCGCCTGGCGCGCCGGCTTTGCACTTGCAAGCGCGAGGGCGACCTGGCGCCGCAGGTGCTGATCGAC
GCTGGCTTCCAGGAATCAGACCTCGACGGAACATGGAAGCCCTATCACCCGGTGGGCTGCGACCGATGCAGCGGTAGCGG
CTACAAGGGGCGATGCGGTATCTACCAGGTCATGCCGATTACCGAAACCATTCAACAGATCATCCTGTCGCACGGCACGG
CATTGCAGATTGCTGAACAGGCGCGCAAGGAAGGCGTGCTATCGTTGCGGGAGGCTGGCCTGCTCAAGGTCAAACAGGGC
GTCACGTCACTCGAAGAAGTGCTGGCGACCACGAACACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(186-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0XA98

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.444

94.241

0.513
  pilB Acinetobacter baumannii D1279779

51.767

98.778

0.511
  pilB Acinetobacter baylyi ADP1

50.906

96.335

0.49
  pilB Legionella pneumophila strain ERS1305867

49.02

97.906

0.48
  pilB Vibrio cholerae strain A1552

45.133

98.604

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

46.642

93.543

0.436
  pilB Vibrio campbellii strain DS40M4

45.756

94.59

0.433
  pilF Thermus thermophilus HB27

38.103

100

0.386

Multiple sequence alignment