Detailed information NTG0347853

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: EXZ61_RS16300
• Genome accession: NZ_CP036282
• Coordinates: 3538842..3540575 (+)
• Length: 577 a.a.
• NCBI ID: WP_142812765.1
• Uniprot ID: -
• Organism: Rhodoferax aquaticus strain Gr-4
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3534630..3553286	3538842..3540575	within	0

Gene organization within MGE regions

Location: 3534630..3553286

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXZ61_RS16275 (EXZ61_16285)	proB	3534630..3535766 (-)	1137	WP_142812761.1	glutamate 5-kinase	-
EXZ61_RS16280 (EXZ61_16290)	cgtA	3535821..3536903 (-)	1083	WP_142812762.1	Obg family GTPase CgtA	-
EXZ61_RS16285 (EXZ61_16295)	rpmA	3536975..3537232 (-)	258	WP_142812763.1	50S ribosomal protein L27	-
EXZ61_RS16290 (EXZ61_16300)	rplU	3537245..3537556 (-)	312	WP_142812764.1	50S ribosomal protein L21	-
EXZ61_RS16295 (EXZ61_16305)	-	3537769..3538698 (+)	930	WP_142814281.1	polyprenyl synthetase family protein	-
EXZ61_RS16300 (EXZ61_16310)	pilB	3538842..3540575 (+)	1734	WP_142812765.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EXZ61_RS16305 (EXZ61_16315)	pilC	3540594..3541811 (+)	1218	WP_142812766.1	type II secretion system F family protein	Machinery gene
EXZ61_RS16310 (EXZ61_16320)	pilD	3541811..3542653 (+)	843	WP_142814282.1	A24 family peptidase	Machinery gene
EXZ61_RS16315 (EXZ61_16325)	coaE	3542654..3543268 (+)	615	WP_142812767.1	dephospho-CoA kinase	-
EXZ61_RS16320 (EXZ61_16330)	zapD	3543291..3544046 (+)	756	WP_142814283.1	cell division protein ZapD	-
EXZ61_RS16325 (EXZ61_16335)	-	3544043..3544252 (+)	210	WP_142812768.1	DNA gyrase inhibitor YacG	-
EXZ61_RS16330 (EXZ61_16340)	-	3544333..3544794 (-)	462	WP_142812769.1	NUDIX domain-containing protein	-
EXZ61_RS16335 (EXZ61_16345)	-	3544799..3545680 (-)	882	WP_142812770.1	ATP-binding protein	-
EXZ61_RS16340 (EXZ61_16350)	argJ	3545694..3546923 (-)	1230	WP_142812771.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
EXZ61_RS16345 (EXZ61_16355)	secA	3546933..3549698 (-)	2766	WP_142812772.1	preprotein translocase subunit SecA	-
EXZ61_RS16350 (EXZ61_16360)	-	3549901..3550836 (-)	936	WP_142812773.1	M23 family metallopeptidase	-
EXZ61_RS22200 (EXZ61_16365)	-	3550861..3551175 (+)	315	WP_142812774.1	hypothetical protein	-
EXZ61_RS16365 (EXZ61_16375)	-	3551327..3551803 (-)	477	WP_142812775.1	ClpXP protease specificity-enhancing factor	-
EXZ61_RS16370 (EXZ61_16380)	-	3551812..3552423 (-)	612	WP_142812776.1	glutathione S-transferase N-terminal domain-containing protein	-
EXZ61_RS16375 (EXZ61_16385)	-	3552528..3553286 (-)	759	WP_142812777.1	cytochrome c1	-

Sequence

Protein

• Length: 577 a.a.
• Molecular weight: 62386.62 Da
• Isoelectric Point: 5.7958

>NTDB_id=347853 EXZ61_RS16300 WP_142812765.1 3538842..3540575(+) (pilB) [Rhodoferax aquaticus strain Gr-4]
MSSAETVNRDTAAIALPGLGRALVAAGKLGQKSAEDVYKKALSGKNSFIGELVGSGAVSARDLAHTLSLTFAAPLIDLDA
IDTQRLPKGILDSKICTDYRVVVLSKRGNRLIVATADPSDQGANEKIKFSTQLGVDWVIAEFDKLSKLVDAANTTASEAM
EDIIGGDFEFDESAAEVESENTQANTAEVEDAPVVRFLHKMLMDAFGMRASDLHFEPYEHTYRVRFRIDGELREIASPPI
AIKDKLASRIKVISRMDISEKRIPQDGRMKLKVGADRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPVEKER
LLTAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNDKAGLTFAAALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPATLTRMRNMGIAPFNIASSVILITAQRLARRLCLNCKAPTDVPA
QTLIDAGFQESDLDGSWHPYRPVGCSACNNGYKGRLGIYQVMPITEEIQKIILADGSALEIAKQAEKEGVRSLRQSGLYK
VKLGLTSLEEVLAVSNL

Nucleotide

• Length: 1734 bp

>NTDB_id=347853 EXZ61_RS16300 WP_142812765.1 3538842..3540575(+) (pilB) [Rhodoferax aquaticus strain Gr-4]
ATGTCCTCAGCCGAAACAGTTAACCGCGATACCGCTGCAATTGCCTTGCCAGGACTCGGTAGAGCGTTGGTTGCAGCCGG
CAAACTCGGGCAAAAGTCGGCGGAGGACGTTTATAAGAAAGCATTGTCAGGAAAAAACAGTTTCATTGGCGAGTTAGTTG
GCTCTGGCGCGGTTTCAGCACGAGACTTGGCGCACACGTTGTCGCTGACCTTCGCTGCGCCTTTGATTGACCTGGATGCC
ATAGACACCCAACGACTTCCAAAAGGCATTTTGGACTCCAAAATTTGCACCGACTACCGGGTCGTAGTCCTGAGTAAAAG
AGGCAATCGGCTTATCGTTGCGACGGCCGACCCGTCTGATCAAGGCGCGAATGAAAAAATCAAGTTCTCGACCCAGTTGG
GTGTCGACTGGGTCATTGCAGAATTTGATAAGTTATCGAAGTTAGTCGATGCGGCCAACACCACCGCAAGCGAAGCGATG
GAAGACATCATTGGTGGCGACTTTGAATTCGATGAAAGCGCCGCCGAGGTTGAATCAGAAAACACCCAAGCGAATACTGC
AGAAGTTGAGGATGCGCCCGTTGTACGTTTTCTTCACAAAATGCTAATGGACGCTTTTGGAATGCGCGCCTCCGACCTGC
ATTTTGAACCTTACGAACACACCTACAGGGTGCGCTTTCGCATCGATGGTGAATTGCGTGAAATAGCGAGTCCCCCCATT
GCGATTAAGGACAAGCTCGCGTCGCGCATCAAGGTGATATCCCGTATGGATATCTCTGAAAAGAGAATTCCCCAAGATGG
CCGCATGAAGCTCAAAGTGGGGGCTGACCGTGTGATTGATTTTCGTGTGAGCACACTACCCACTTTGTTTGGTGAAAAAA
TAGTGATCCGTATTCTCGATCCGAGTAGCGCCAAACTGGGGATTGATGCGCTTGGTTACGAACCAGTAGAAAAAGAACGC
TTACTGACAGCCATTGGTCGCCCTTACGGAATGATCTTAGTAACAGGACCAACTGGCTCAGGTAAGACGGTCTCTTTGTA
TACATGCTTGAACTTGTTGAATAAGCCGGGTGTCAATATAGCGACAGCTGAGGATCCATCTGAAATCAACCTTCCTGGGG
TTAATCAGGTTAACGTCAATGATAAGGCTGGGCTTACCTTTGCAGCCGCACTTAAATCATTTCTGCGCCAGGATCCTGAC
ATTATTATGGTTGGTGAAATTCGCGACTTAGAGACAGCCGACATCTCAATCAAGGCAGCGCAGACGGGACATTTGGTGCT
CTCTACGCTGCATACCAATGACGCTCCCGCGACACTCACTCGCATGCGCAATATGGGTATTGCACCCTTTAACATTGCCT
CTAGCGTCATACTTATCACTGCACAGCGCTTGGCGCGTCGTTTGTGTCTCAATTGCAAAGCACCGACCGACGTACCAGCA
CAAACCTTGATTGATGCGGGATTTCAAGAATCCGACTTAGATGGCTCATGGCACCCGTACCGACCCGTAGGCTGTTCGGC
GTGCAATAACGGCTATAAAGGTCGGCTGGGCATCTATCAAGTCATGCCAATCACCGAAGAAATTCAAAAAATCATATTGG
CGGATGGCAGTGCCCTAGAAATTGCTAAACAGGCTGAAAAAGAAGGGGTCCGATCTTTACGACAGTCGGGCTTATACAAA
GTGAAGCTTGGGCTAACTTCCTTGGAAGAAGTACTTGCAGTTTCCAACCTCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (200-467)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (52-151)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	54.707	97.574	0.534
pilB	Acinetobacter baylyi ADP1	53.982	97.92	0.529
pilF	Neisseria gonorrhoeae MS11	53.737	97.4	0.523
pilB	Legionella pneumophila strain ERS1305867	51.779	97.4	0.504
pilB	Vibrio campbellii strain DS40M4	44.444	98.267	0.437
pilB	Vibrio parahaemolyticus RIMD 2210633	46.455	92.894	0.432
pilB	Vibrio cholerae strain A1552	47.184	89.255	0.421
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.33	98.267	0.386
pilF	Thermus thermophilus HB27	38.179	98.96	0.378