Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW59_RS26250 Genome accession   NZ_CP036084
Coordinates   4218145..4219188 (-) Length   347 a.a.
NCBI ID   WP_002014996.1    Uniprot ID   R8I3I9
Organism   Bacillus mycoides strain JAS1004     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4213145..4224188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW59_RS26205 (EXW59_26790) - 4213544..4213744 (-) 201 WP_002015009.1 YqzE family protein -
  EXW59_RS26210 (EXW59_26795) aroK 4213783..4214280 (-) 498 WP_098635944.1 shikimate kinase AroK -
  EXW59_RS26215 (EXW59_26800) - 4214432..4215082 (-) 651 WP_098635943.1 2OG-Fe(II) oxygenase -
  EXW59_RS26220 (EXW59_26805) comGG 4215256..4215627 (-) 372 WP_061674785.1 competence type IV pilus minor pilin ComGG -
  EXW59_RS26225 (EXW59_26810) comGF 4215624..4216085 (-) 462 WP_002088620.1 competence type IV pilus minor pilin ComGF -
  EXW59_RS26230 (EXW59_26815) - 4216069..4216365 (-) 297 WP_002088621.1 hypothetical protein -
  EXW59_RS26235 (EXW59_26820) comGD 4216358..4216813 (-) 456 WP_002088622.1 competence type IV pilus minor pilin ComGD -
  EXW59_RS26240 (EXW59_26825) comGC 4216810..4217109 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  EXW59_RS26245 (EXW59_26830) comGB 4217121..4218158 (-) 1038 WP_002138336.1 competence type IV pilus assembly protein ComGB -
  EXW59_RS26250 (EXW59_26835) comGA 4218145..4219188 (-) 1044 WP_002014996.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW59_RS26255 (EXW59_26840) - 4219394..4220089 (+) 696 WP_078172814.1 helix-turn-helix domain-containing protein -
  EXW59_RS26260 (EXW59_26845) - 4220218..4220460 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW59_RS26265 (EXW59_26850) - 4220568..4221962 (+) 1395 WP_002138338.1 L-cystine transporter -
  EXW59_RS26270 (EXW59_26855) - 4222043..4222435 (-) 393 WP_098635942.1 hypothetical protein -
  EXW59_RS26275 (EXW59_26860) - 4222523..4222738 (-) 216 WP_002015115.1 DUF3912 family protein -
  EXW59_RS26280 (EXW59_26865) - 4222991..4223302 (+) 312 WP_002165070.1 hypothetical protein -
  EXW59_RS26285 (EXW59_26870) - 4223338..4223826 (-) 489 WP_002015119.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39264.73 Da        Isoelectric Point: 9.3368

>NTDB_id=346541 EXW59_RS26250 WP_002014996.1 4218145..4219188(-) (comGA) [Bacillus mycoides strain JAS1004]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=346541 EXW59_RS26250 WP_002014996.1 4218145..4219188(-) (comGA) [Bacillus mycoides strain JAS1004]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8I3I9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

43.151

84.15

0.363

Multiple sequence alignment