Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GE296_RS06185 Genome accession   NZ_CP045564
Coordinates   1325962..1326918 (+) Length   318 a.a.
NCBI ID   WP_004916143.1    Uniprot ID   E4TAL4
Organism   Riemerella anatipestifer strain RA-LZ01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1320962..1331918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GE296_RS06155 - 1321435..1322079 (+) 645 WP_004916137.1 DUF4271 domain-containing protein -
  GE296_RS06160 - 1322102..1322845 (+) 744 WP_013447024.1 uroporphyrinogen-III synthase -
  GE296_RS06165 - 1322937..1324985 (+) 2049 WP_004916139.1 S9 family peptidase -
  GE296_RS06170 rpmB 1325260..1325496 (+) 237 WP_004916140.1 50S ribosomal protein L28 -
  GE296_RS06175 rpmG 1325507..1325689 (+) 183 WP_004916141.1 50S ribosomal protein L33 -
  GE296_RS06180 - 1325712..1325861 (+) 150 WP_004916142.1 DUF4295 family protein -
  GE296_RS06185 pilA 1325962..1326918 (+) 957 WP_004916143.1 signal recognition particle-docking protein FtsY Machinery gene
  GE296_RS06190 - 1326959..1327213 (+) 255 WP_004916144.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  GE296_RS06195 topA 1327400..1329919 (+) 2520 WP_004916145.1 type I DNA topoisomerase -
  GE296_RS06200 - 1329931..1330995 (+) 1065 WP_004916146.1 formimidoylglutamase -

Sequence

Protein


Download         Length: 318 a.a.        Molecular weight: 34733.97 Da        Isoelectric Point: 6.0732

>NTDB_id=345146 GE296_RS06185 WP_004916143.1 1325962..1326918(+) (pilA) [Riemerella anatipestifer strain RA-LZ01]
MSWFKKIFKKEEKETLDRGLEKSSQGFFEKISKAVIGKSKVDDEVLDDLEEVLIASDVGAQTTIKIIERIEDRVARDKYV
GVDELHKLLKEEITALLLENPHADTGNIDEAKKPYVIMVVGVNGVGKTTTIGKLAHQFKTQGKKVVLGAADTFRAAAVDQ
LVIWSERVGVPIVKQNMGSDPASVAFDTVQSAVAQNADVVLIDTAGRLHNKVNLMNELSKIKRVMQKVIPDAPHEVLLVL
DGSTGQNAFEQAKQFTAATEVNALAVTKLDGTAKGGVVIGISDQFQVPVKYIGVGEKMEDLQLFNGTEFVDSLFKKKS

Nucleotide


Download         Length: 957 bp        

>NTDB_id=345146 GE296_RS06185 WP_004916143.1 1325962..1326918(+) (pilA) [Riemerella anatipestifer strain RA-LZ01]
ATGAGTTGGTTTAAAAAAATATTCAAAAAAGAAGAGAAGGAAACTTTAGATAGAGGGTTAGAAAAGTCAAGTCAAGGCTT
TTTTGAAAAAATATCCAAAGCAGTCATAGGGAAAAGTAAGGTAGATGATGAAGTTTTAGATGATTTGGAAGAGGTTCTTA
TTGCATCAGATGTAGGGGCTCAGACAACTATCAAGATTATCGAACGAATTGAAGATAGAGTTGCTAGGGATAAGTATGTT
GGTGTAGATGAACTACATAAGCTTCTTAAAGAAGAAATTACAGCATTGCTTTTAGAAAATCCTCATGCTGATACGGGGAA
TATAGATGAGGCTAAAAAACCTTACGTAATAATGGTGGTGGGAGTCAATGGAGTAGGTAAAACAACTACGATAGGTAAAT
TAGCACATCAGTTTAAAACACAGGGTAAAAAAGTGGTGCTAGGAGCAGCAGATACTTTTAGAGCGGCGGCGGTGGACCAG
CTGGTGATATGGAGTGAGCGAGTAGGTGTACCTATTGTAAAGCAAAATATGGGTTCTGACCCTGCTTCTGTTGCCTTTGA
TACAGTGCAATCTGCAGTAGCTCAAAATGCAGATGTAGTACTTATAGATACAGCTGGGAGGCTTCATAATAAAGTAAATC
TAATGAATGAATTGTCTAAGATAAAAAGAGTAATGCAGAAAGTAATTCCTGATGCTCCACATGAGGTTTTATTGGTATTA
GACGGTTCTACAGGGCAAAATGCATTTGAACAAGCTAAGCAATTTACTGCTGCAACAGAAGTTAATGCTCTTGCGGTTAC
AAAATTAGATGGTACAGCAAAAGGAGGTGTGGTTATTGGTATTTCTGATCAATTTCAAGTTCCTGTAAAATATATAGGGG
TAGGAGAGAAGATGGAAGACTTGCAGTTATTTAATGGCACGGAGTTTGTTGACTCTCTCTTCAAGAAAAAGAGTTGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(115-314)

SRP54-type protein, helical bundle domain (SRP54_N)

(28-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4TAL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

45.515

94.654

0.431

Multiple sequence alignment