Detailed information NTG0345146

Bioinformatically predicted

Overview

• Name: pilO
• Type: Machinery gene
• Locus tag: C9E88_RS01700
• Genome accession: NZ_CP035934
• Coordinates: 374098..374796 (+)
• Length: 232 a.a.
• NCBI ID: WP_106983929.1
• Uniprot ID: A0A7Y8PZL1
• Organism: Acinetobacter cumulans strain WCHAc060092
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 369098..379796

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C9E88_RS01685 (C9E88_002585)	ponA	369697..372225 (-)	2529	WP_106983926.1	penicillin-binding protein PBP1a	-
C9E88_RS01690 (C9E88_002580)	comM	372390..373448 (+)	1059	WP_106983927.1	pilus assembly protein PilM	Machinery gene
C9E88_RS01695 (C9E88_002575)	pilN	373448..374101 (+)	654	WP_106983928.1	PilN domain-containing protein	Machinery gene
C9E88_RS01700 (C9E88_002570)	pilO	374098..374796 (+)	699	WP_106983929.1	type 4a pilus biogenesis protein PilO	Machinery gene
C9E88_RS01705 (C9E88_002565)	pilP	374796..375323 (+)	528	WP_106983930.1	pilus assembly protein PilP	Machinery gene
C9E88_RS01710 (C9E88_002560)	pilQ	375340..377430 (+)	2091	WP_106983931.1	type IV pilus secretin PilQ family protein	Machinery gene
C9E88_RS01715 (C9E88_002555)	-	377518..378018 (+)	501	WP_240627350.1	shikimate kinase	-
C9E88_RS01720 (C9E88_002550)	aroB	378032..379111 (+)	1080	WP_106983933.1	3-dehydroquinate synthase	-

Sequence

Protein

• Length: 232 a.a.
• Molecular weight: 26325.80 Da
• Isoelectric Point: 4.8418

>NTDB_id=345146 C9E88_RS01700 WP_106983929.1 374098..374796(+) (pilO) [Acinetobacter cumulans strain WCHAc060092]
MSREEFDELNQASVAPAKKKMTLDKFLQQFNTLDPNNYGSWPLSVKITCWIFIVLLVLALAYFVMIKGKIESIANANAQE
QNLLNEFREKESKLRNLQQYQTQLQDMEARFNQQLDQLPKETEIPGLVEDINVTGVNSGLKFKNIRLEPEVKQEFFIEQP
ISMEATGDYHAFGSFVSGIAGLSRIVTLHDFTISSSENPQKKSEIPSINYTLQAKTYRYVGNSDNASTEGAQ

Nucleotide

• Length: 699 bp

>NTDB_id=345146 C9E88_RS01700 WP_106983929.1 374098..374796(+) (pilO) [Acinetobacter cumulans strain WCHAc060092]
ATGAGTCGTGAAGAATTTGATGAATTAAATCAAGCATCTGTTGCGCCCGCAAAGAAAAAAATGACGCTGGATAAATTCTT
ACAACAATTCAATACTTTGGATCCAAATAACTATGGTAGTTGGCCCTTATCGGTCAAAATTACTTGTTGGATTTTTATTG
TACTGCTGGTATTAGCGTTAGCTTATTTTGTCATGATCAAAGGCAAAATTGAATCAATCGCAAATGCAAATGCACAAGAA
CAAAACTTATTAAATGAATTCCGTGAAAAAGAATCTAAGTTGCGTAATTTACAACAATATCAAACTCAATTGCAGGATAT
GGAGGCGCGTTTTAATCAGCAATTGGATCAATTGCCGAAAGAAACTGAAATTCCAGGTTTGGTTGAAGATATCAACGTAA
CAGGTGTGAATTCGGGGTTGAAGTTTAAGAATATTCGACTTGAACCGGAAGTAAAGCAAGAGTTCTTTATTGAACAGCCG
ATTTCGATGGAAGCAACTGGTGATTATCACGCCTTTGGTAGTTTTGTAAGTGGAATTGCAGGATTATCTCGTATTGTCAC
TTTGCATGATTTTACGATTAGCTCTTCTGAAAATCCTCAGAAAAAATCTGAAATTCCAAGCATAAATTATACTCTTCAGG
CCAAAACTTATCGTTATGTAGGTAATAGTGATAATGCATCCACGGAAGGAGCACAATAA

Domains

Predicted by InterproScan.

• Pilus assembly protein, PilO (PilO) (73-220)

3D structure

• AlphaFold DB: A0A7Y8PZL1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilO	Acinetobacter baumannii D1279779	70.435	99.138	0.698
comO	Acinetobacter baylyi ADP1	66.379	100	0.664