Detailed information    

insolico Bioinformatically predicted

Overview

Name   htrA   Type   Regulator
Locus tag   EWM54_RS01780 Genome accession   NZ_CP035654
Coordinates   276704..277993 (-) Length   429 a.a.
NCBI ID   WP_002290101.1    Uniprot ID   A0A828ZUK2
Organism   Enterococcus faecium strain UAMSEF_08     
Function   repress competence development (predicted from homology)   
Competence regulation

Genomic Context

Location: 271704..282993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWM54_RS01745 (EWM54_01740) - 272148..273101 (-) 954 WP_002299300.1 glycosyltransferase family 2 protein -
  EWM54_RS01750 (EWM54_01745) - 273286..273636 (-) 351 WP_002309710.1 PepSY domain-containing protein -
  EWM54_RS01755 (EWM54_01750) pepA 273836..274915 (+) 1080 WP_002294156.1 glutamyl aminopeptidase -
  EWM54_RS01760 (EWM54_01755) - 275058..275378 (+) 321 WP_002292632.1 thioredoxin family protein -
  EWM54_RS01765 (EWM54_01760) - 275400..275864 (+) 465 WP_002345012.1 universal stress protein -
  EWM54_RS01775 (EWM54_01770) ytpR 276060..276665 (+) 606 WP_002313997.1 YtpR family tRNA-binding protein -
  EWM54_RS01780 (EWM54_01775) htrA 276704..277993 (-) 1290 WP_002290101.1 trypsin-like peptidase domain-containing protein Regulator
  EWM54_RS01785 (EWM54_01780) rlmH 278421..278900 (+) 480 WP_002345011.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  EWM54_RS01795 (EWM54_01790) - 279253..279567 (+) 315 WP_000420682.1 YdcP family protein -
  EWM54_RS01800 (EWM54_01795) - 279583..279969 (+) 387 WP_000985015.1 YdcP family protein -
  EWM54_RS01805 (EWM54_01800) - 279998..281383 (+) 1386 WP_000813488.1 FtsK/SpoIIIE domain-containing protein -
  EWM54_RS01810 (EWM54_01805) - 281386..281539 (+) 154 Protein_278 conjugal transfer protein -
  EWM54_RS01815 (EWM54_01810) mobT 281562..282767 (+) 1206 WP_000398284.1 MobT family relaxase -

Sequence

Protein


Download         Length: 429 a.a.        Molecular weight: 44916.93 Da        Isoelectric Point: 4.5419

>NTDB_id=343368 EWM54_RS01780 WP_002290101.1 276704..277993(-) (htrA) [Enterococcus faecium strain UAMSEF_08]
MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAMGTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKA
VDKVQDAVVSVINLQSQNQSSGFGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYANSVTSGIISSLNRQVTSTNESNE
TVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSKIASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALG
ITMVDLSAVSTQQQEQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKKVGDSVKIT
YYEGSKEKTTTVELTIDQSALKQNQNSEN

Nucleotide


Download         Length: 1290 bp        

>NTDB_id=343368 EWM54_RS01780 WP_002290101.1 276704..277993(-) (htrA) [Enterococcus faecium strain UAMSEF_08]
ATGGATAGAAAAAATGTGACACCAAAGATGAAAAAAAATAAAAATAATAGCCTCTGGCGTAAGTTGGGTCTGGGGCTTGT
TGGAGGGATCGTTGGAGGACTACTGACAGCTGGCATTTTTTATGCAGCGATGGGCACTGGCAACAATAGTTCTACTGCTA
CAAGCGGCAATCAAAATTCTGCCGGTGAAACAGTTGTGGAAAATGTCAAAGTCAATGTAGATTCTGATATCACCAAAGCG
GTAGATAAAGTTCAAGACGCTGTCGTTTCTGTTATCAACTTACAAAGTCAAAACCAATCAAGCGGTTTTGGCGGATTATT
CGGGCAGCAAGAAGAAAGTAGCAGCTCAGATGATAGTAATTTGGAAGCTTATAGTGAAGGAAGCGGCGTAATCTACAAAA
AAGACGGGAATACTGCTTATGTAGTCACAAACAATCACGTAGTAGATGGACAGCAAGGTTTGGAAGTCATGATGAAAGAC
GGTACGAAGGTAAAAGCTGAATTAGTTGGAACAGATGCCTATTCAGATTTAGCGGTGTTGAAGATCAATTCAGACAAAGT
CGAAACGGTTGCCTCTTTTGGGGACTCAAGTGCTTTGAAGGTTGGTGAACCTGCGATTGCGATTGGTTCCCCATTAGGAT
CTGAATATGCGAATTCTGTCACTTCAGGAATCATTTCTTCCTTGAACCGACAAGTAACAAGTACGAATGAATCGAACGAA
ACAGTCAATATCAATGCGATCCAAACAGATGCAGCGATCAACCCGGGAAATTCTGGTGGTCCTTTAGTGAATATCGAAGG
ACAAGTTATCGGTATCAACTCAAGTAAGATCGCAAGTACTTCTGAATCGACTTCTAATGTAAGCGTAGAAGGAATGGGAT
TTGCCATCCCAAGTAACGATGTAGTGAATATCATCAACCAACTGGAAAAAGACGGAAAAGTGACACGTCCAGCTCTAGGT
ATCACAATGGTCGACTTATCTGCCGTTTCTACTCAGCAACAAGAACAAATCTTGAAGATTCCTTCATCTGTAACTAACGG
TGTCATCGTGACAAATGTCCAAACAGCTACACCGGCAGAAAAAGCTGGGTTGAAACAATACGACGTCATTACTAAGATCG
ATGACAAAGAAGTTTCTTCTGGTGTAGAACTTCAATCTGCTTTGTATAAGAAAAAAGTGGGAGATTCTGTCAAAATCACT
TACTATGAAGGCTCGAAAGAAAAAACAACTACTGTTGAATTGACGATCGATCAATCGGCATTAAAACAAAATCAAAATAG
TGAAAATTAA

Domains

Predicted by InterproScan.

Peptidase family M50 (Peptidase_M50)

(10-417)

Trypsin-like peptidase domain (Trypsin_2)

(127-272)

PDZ domain (PDZ_2)

(317-414)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A828ZUK2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus gordonii str. Challis substr. CH1

54.569

91.841

0.501
  htrA Streptococcus mutans UA159

60.169

82.517

0.496
  htrA Streptococcus mitis NCTC 12261

52.778

92.308

0.487
  htrA Streptococcus pneumoniae TIGR4

59.05

78.555

0.464
  htrA Streptococcus pneumoniae Rx1

59.05

78.555

0.464
  htrA Streptococcus pneumoniae D39

59.05

78.555

0.464
  htrA Streptococcus pneumoniae R6

59.05

78.555

0.464

Multiple sequence alignment