Detailed information NTG0343000

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EU243_RS11455
• Genome accession: NZ_CP035533
• Coordinates: 2241939..2242553 (+)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain DTU001
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2236939..2247553

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EU243_RS11420 (EU243_11610)	aroE	2237627..2238469 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
EU243_RS11425 (EU243_11615)	yhbY	2238463..2238753 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
EU243_RS11430 (EU243_11620)	-	2238764..2239332 (+)	569	Protein_2208	nicotinate-nucleotide adenylyltransferase	-
EU243_RS11435 (EU243_11625)	yqeK	2239322..2239882 (+)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EU243_RS11440 (EU243_11630)	rsfS	2239900..2240256 (+)	357	WP_003152864.1	ribosome silencing factor	-
EU243_RS11445 (EU243_11635)	-	2240253..2240990 (+)	738	WP_032856885.1	class I SAM-dependent DNA methyltransferase	-
EU243_RS11450 (EU243_11640)	comER	2241059..2241880 (-)	822	WP_014305449.1	late competence protein ComER	-
EU243_RS11455 (EU243_11645)	comEA	2241939..2242553 (+)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
EU243_RS11460 (EU243_11650)	-	2242620..2243189 (+)	570	WP_003152868.1	ComE operon protein 2	-
EU243_RS11465 (EU243_11655)	-	2243190..2245540 (+)	2351	Protein_2215	DNA internalization-related competence protein ComEC/Rec2	-
EU243_RS11470 (EU243_11660)	-	2245559..2245693 (-)	135	WP_003152870.1	YqzM family protein	-
EU243_RS11475 (EU243_11665)	-	2245734..2245888 (+)	155	Protein_2217	hypothetical protein	-
EU243_RS11480 (EU243_11670)	holA	2245928..2246969 (+)	1042	Protein_2218	DNA polymerase III subunit delta	-
EU243_RS11485 (EU243_11675)	rpsT	2246986..2247252 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=343000 EU243_RS11455 WP_041482377.1 2241939..2242553(+) (comEA) [Bacillus velezensis strain DTU001]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=343000 EU243_RS11455 WP_041482377.1 2241939..2242553(+) (comEA) [Bacillus velezensis strain DTU001]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363