Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   ETT46_RS00665 Genome accession   NZ_CP035453
Coordinates   102837..103871 (+) Length   344 a.a.
NCBI ID   WP_227877282.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm100     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97837..108871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT46_RS00655 (ETT46_00655) - 101490..101855 (+) 366 WP_002986560.1 DUF1033 family protein -
  ETT46_RS00660 (ETT46_00660) comYA 101948..102901 (+) 954 WP_030126921.1 competence type IV pilus ATPase ComGA Machinery gene
  ETT46_RS00665 (ETT46_00665) comYB 102837..103871 (+) 1035 WP_227877282.1 competence type IV pilus assembly protein ComGB Machinery gene
  ETT46_RS00670 (ETT46_00670) comYC 103873..104199 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  ETT46_RS00675 (ETT46_00675) comGD 104174..104602 (+) 429 WP_023605232.1 competence type IV pilus minor pilin ComGD -
  ETT46_RS00680 (ETT46_00680) comGE 104559..104843 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  ETT46_RS00685 (ETT46_00685) comYF 104836..105270 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  ETT46_RS00690 (ETT46_00690) comGG 105254..105580 (+) 327 WP_002992739.1 competence type IV pilus minor pilin ComGG -
  ETT46_RS00695 (ETT46_00695) comYH 105678..106631 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  ETT46_RS00700 (ETT46_00700) - 106690..107886 (+) 1197 WP_109821056.1 acetate kinase -
  ETT46_RS00705 (ETT46_00705) - 108073..108381 (+) 309 WP_032467031.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39632.10 Da        Isoelectric Point: 9.7085

>NTDB_id=342381 ETT46_RS00665 WP_227877282.1 102837..103871(+) (comYB) [Streptococcus pyogenes strain emm100]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMAILDIMAIEKS
SLMKELGEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=342381 ETT46_RS00665 WP_227877282.1 102837..103871(+) (comYB) [Streptococcus pyogenes strain emm100]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGGCTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGGTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

54.277

98.547

0.535
  comYB Streptococcus mutans UA159

54.277

98.547

0.535
  comYB Streptococcus gordonii str. Challis substr. CH1

50.882

98.837

0.503
  comGB/cglB Streptococcus mitis NCTC 12261

47.917

97.674

0.468
  comGB/cglB Streptococcus mitis SK321

48.048

96.802

0.465
  comGB/cglB Streptococcus pneumoniae Rx1

45.946

96.802

0.445
  comGB/cglB Streptococcus pneumoniae D39

45.946

96.802

0.445
  comGB/cglB Streptococcus pneumoniae R6

45.946

96.802

0.445
  comGB/cglB Streptococcus pneumoniae TIGR4

45.946

96.802

0.445
  comGB Lactococcus lactis subsp. cremoris KW2

43.077

94.477

0.407

Multiple sequence alignment