Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   ETT47_RS00665 Genome accession   NZ_CP035452
Coordinates   102719..103753 (+) Length   344 a.a.
NCBI ID   WP_227868940.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm123     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97719..108753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT47_RS00655 (ETT47_00655) - 101387..101752 (+) 366 WP_002986560.1 DUF1033 family protein -
  ETT47_RS00660 (ETT47_00660) comYA 101845..102783 (+) 939 WP_002993471.1 competence type IV pilus ATPase ComGA Machinery gene
  ETT47_RS00665 (ETT47_00665) comYB 102719..103753 (+) 1035 WP_227868940.1 competence type IV pilus assembly protein ComGB Machinery gene
  ETT47_RS00670 (ETT47_00670) comYC 103755..104081 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  ETT47_RS00675 (ETT47_00675) comGD 104056..104484 (+) 429 WP_109991901.1 competence type IV pilus minor pilin ComGD -
  ETT47_RS00680 (ETT47_00680) comGE 104441..104725 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  ETT47_RS00685 (ETT47_00685) comYF 104718..105152 (+) 435 WP_109991902.1 competence type IV pilus minor pilin ComGF Machinery gene
  ETT47_RS00690 (ETT47_00690) comGG 105136..105462 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  ETT47_RS00695 (ETT47_00695) comYH 105560..106513 (+) 954 WP_136038165.1 class I SAM-dependent methyltransferase Machinery gene
  ETT47_RS00700 (ETT47_00700) - 106572..107768 (+) 1197 WP_030126459.1 acetate kinase -
  ETT47_RS00705 (ETT47_00705) - 107954..108262 (+) 309 Protein_89 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39734.23 Da        Isoelectric Point: 9.7085

>NTDB_id=342329 ETT47_RS00665 WP_227868940.1 102719..103753(+) (comYB) [Streptococcus pyogenes strain emm123]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMVRIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQTFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=342329 ETT47_RS00665 WP_227868940.1 102719..103753(+) (comYB) [Streptococcus pyogenes strain emm123]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGTAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGTCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAACCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.982

98.547

0.532
  comYB Streptococcus mutans UA159

53.982

98.547

0.532
  comYB Streptococcus gordonii str. Challis substr. CH1

50.294

98.837

0.497
  comGB/cglB Streptococcus mitis NCTC 12261

47.321

97.674

0.462
  comGB/cglB Streptococcus mitis SK321

47.447

96.802

0.459
  comGB/cglB Streptococcus pneumoniae Rx1

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae D39

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae R6

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae TIGR4

45.345

96.802

0.439
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment