Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   ETT60_RS06705 Genome accession   NZ_CP035439
Coordinates   1299511..1301409 (-) Length   632 a.a.
NCBI ID   WP_012560855.1    Uniprot ID   A0A5S4TNG2
Organism   Streptococcus pyogenes strain emm77     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 1294511..1306409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT60_RS06680 (ETT60_06670) - 1295247..1295624 (-) 378 WP_002991882.1 S1 RNA-binding domain-containing protein -
  ETT60_RS06685 (ETT60_06675) - 1295624..1297024 (-) 1401 WP_023079249.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  ETT60_RS06690 (ETT60_06680) - 1297061..1297702 (-) 642 WP_002983671.1 response regulator transcription factor -
  ETT60_RS06695 (ETT60_06685) - 1297695..1298699 (-) 1005 WP_002991890.1 sensor histidine kinase -
  ETT60_RS06700 (ETT60_06690) liaF 1298696..1299388 (-) 693 WP_011054833.1 cell wall-active antibiotics response protein LiaF -
  ETT60_RS06705 (ETT60_06695) pknB 1299511..1301409 (-) 1899 WP_012560855.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  ETT60_RS06710 (ETT60_06700) - 1301406..1302146 (-) 741 WP_002983660.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ETT60_RS06715 (ETT60_06705) rsmB 1302184..1303506 (-) 1323 WP_032465161.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ETT60_RS06720 (ETT60_06710) fmt 1303496..1304431 (-) 936 WP_129284318.1 methionyl-tRNA formyltransferase -

Sequence

Protein


Download         Length: 632 a.a.        Molecular weight: 68833.09 Da        Isoelectric Point: 7.2899

>NTDB_id=341657 ETT60_RS06705 WP_012560855.1 1299511..1301409(-) (pknB) [Streptococcus pyogenes strain emm77]
MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVLRTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIG
EEDGQQFLVMEYVDGADLKRYIQNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSIIEENHNVP
QALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYNRSRERKIIFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESR
LDQTNQTDALQPPTKKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDVGLKVGKIR
QIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDYQEAMNSLIETYGVPKSKIKIERIVTNEYPE
NTVISQSPSAGDKFNPNGKSKITLSVAVSDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSS
KAIVSGQSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSPSETTAQTP

Nucleotide


Download         Length: 1899 bp        

>NTDB_id=341657 ETT60_RS06705 WP_012560855.1 1299511..1301409(-) (pknB) [Streptococcus pyogenes strain emm77]
ATGATTCAGATTGGCAAATTATTTGCTGGTCGTTATCGCATTCTGAAATCTATTGGCCGCGGTGGTATGGCGGATGTTTA
TTTAGCAAATGATTTGATCTTGGATAATGAAGACGTTGCAATCAAGGTCTTGCGTACCAATTATCAAACAGATCAGGTAG
CAGTTGCGCGTTTCCAACGAGAAGCGCGGGCCATGGCTGAATTGAACCATCCCAATATTGTTGCCATCCGGGATATAGGT
GAAGAAGACGGACAGCAATTTTTAGTAATGGAATATGTGGATGGTGCTGACCTAAAGAGATACATTCAAAATCATGCTCC
ATTATCTAATAATGAAGTGGTTAGAATTATGGAAGAAGTCCTTTCTGCTATGACTTTAGCCCACCAAAAAGGAATTGTAC
ACAGAGATTTAAAACCTCAAAATATCCTACTAACTAAGGAGGGTGTTGTCAAAGTAACTGATTTCGGCATCGCAGTAGCC
TTTGCAGAAACAAGCTTGACACAAACTAATTCGATGTTAGGCAGTGTTCATTACTTGTCTCCAGAACAGGCTCGCGGCTC
CAAAGCGACGATTCAAAGTGATATTTATGCGATGGGGATTATGCTCTTTGAGATGTTGACAGGCCATATCCCTTATGATG
GCGATAGTGCTGTTACGATTGCCTTGCAACATTTTCAAAAGCCTCTTCCATCTATTATCGAGGAGAACCACAATGTGCCA
CAAGCTTTGGAGAATGTTGTTATTCGAGCAACAGCCAAGAAATTAAGCGATCGTTACGGGTCAACCTTTGAAATGAGTCG
TGACTTAATGACGGCGCTTAGTTATAATCGTAGTCGGGAGCGTAAGATTATCTTTGAGGATGTTGAAAGTACCAAACCCC
TCCCCAAAGTGGCCTCAGGTCCCACCGCTTCTGTAAAATTGTCTCCCCCTACCCCAACAGTGTTAACACAGGAAAGTCGA
TTAGATCAAACTAATCAAACAGATGCTTTACAGCCCCCCACCAAAAAGAAAAAAAGTGGTCGTTTTTTAGGTACTTTATT
CAAAATTCTTTTTTCTTTCTTTATTGTAGGTGTAGCACTCTTTACTTATCTTATACTAACTAAACCAACTTCTGTGAAAG
TTCCTAATGTAGCAGGCACTAGTCTTAAAGTTGCCAAACAAGAACTGTATGATGTTGGGCTAAAAGTGGGTAAAATCAGG
CAAATTGAGAGTGATACGGTTGCTGAGGGAAATGTAGTTAGAACAGATCCTAAAGCAGGAACAGCTAAGAGGCAAGGCTC
AAGCATTACGCTTTATGTGTCAATTGGAAACAAAGGTTTTGACATGGAAAACTACAAAGGACTAGATTATCAAGAAGCTA
TGAATAGTTTGATAGAAACTTATGGTGTTCCAAAATCAAAAATCAAAATTGAGCGCATTGTAACTAATGAATATCCTGAA
AATACAGTCATCAGTCAATCGCCAAGTGCGGGTGATAAATTTAATCCAAACGGAAAGTCTAAAATTACGCTCAGTGTTGC
TGTTAGTGATACGATCACTATGCCTATGGTAACAGAATATAGTTATGCAGATGCAGTCAATACCTTAACAGCTTTAGGTA
TAGATGCATCTAGAATAAAAGCTTATGTGCCAAGCTCTAGCTCAGCAACCGGCTTTGTGCCAATTCATTCTCCTAGTTCT
AAAGCTATTGTCAGTGGTCAATCTCCTTACTATGGAACGTCTTTGAGTCTGTCTGATAAAGGAGAGATTAGTCTTTACCT
TTATCCAGAAGAAACACACTCTTCTAGTAGCTCATCGAGTTCAACGTCAAGTTCAAACAGTTCTTCAACAAATGATAGTA
CTGCACCAGGTAGCAACACTGAATTAAGCCCATCAGAAACTACTGCTCAAACACCTTAA

Domains

Predicted by InterproScan.

PASTA domain (PASTA)

(372-434)

PASTA domain (PASTA)

(441-506)

PASTA domain (PASTA)

(512-588)

Protein kinase domain (Pkinase)

(12-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TNG2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

65.254

93.354

0.609
  stkP/pknB Streptococcus salivarius strain HSISS4

61.345

94.146

0.578
  stkP Streptococcus pneumoniae TIGR4

57.364

81.646

0.468
  stkP Streptococcus pneumoniae D39

57.171

81.646

0.467
  stkP Streptococcus pneumoniae R6

57.171

81.646

0.467
  stkP Streptococcus pneumoniae Rx1

56.977

81.646

0.465

Multiple sequence alignment