Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   ETT61_RS00735 Genome accession   NZ_CP035438
Coordinates   113726..114760 (+) Length   344 a.a.
NCBI ID   WP_231871599.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm22.8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 108726..119760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT61_RS00725 (ETT61_00725) - 112394..112759 (+) 366 WP_002986560.1 DUF1033 family protein -
  ETT61_RS00730 (ETT61_00730) comYA 112852..113790 (+) 939 WP_023079010.1 competence type IV pilus ATPase ComGA Machinery gene
  ETT61_RS00735 (ETT61_00735) comYB 113726..114760 (+) 1035 WP_231871599.1 competence type IV pilus assembly protein ComGB Machinery gene
  ETT61_RS00740 (ETT61_00740) comYC 114762..115088 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  ETT61_RS00745 (ETT61_00745) comGD 115063..115490 (+) 428 Protein_98 competence type IV pilus minor pilin ComGD -
  ETT61_RS00750 (ETT61_00750) comGE 115447..115731 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  ETT61_RS00755 (ETT61_00755) comYF 115724..116158 (+) 435 WP_136020264.1 competence type IV pilus minor pilin ComGF Machinery gene
  ETT61_RS00760 (ETT61_00760) comGG 116142..116468 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  ETT61_RS00765 (ETT61_00765) comYH 116566..117519 (+) 954 WP_136020265.1 class I SAM-dependent methyltransferase Machinery gene
  ETT61_RS00770 (ETT61_00770) - 117578..118774 (+) 1197 WP_010921803.1 acetate kinase -
  ETT61_RS00775 (ETT61_00775) - 118960..119268 (+) 309 WP_030126458.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39690.18 Da        Isoelectric Point: 9.7085

>NTDB_id=341566 ETT61_RS00735 WP_231871599.1 113726..114760(+) (comYB) [Streptococcus pyogenes strain emm22.8]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLIPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=341566 ETT61_RS00735 WP_231871599.1 113726..114760(+) (comYB) [Streptococcus pyogenes strain emm22.8]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAATCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTTTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCTATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)

Type II secretion system (T2SS), protein F (T2SSF)

(28-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.982

98.547

0.532
  comYB Streptococcus mutans UA159

53.982

98.547

0.532
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment