Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   ETT61_RS00730 Genome accession   NZ_CP035438
Coordinates   112852..113790 (+) Length   312 a.a.
NCBI ID   WP_023079010.1    Uniprot ID   A0A8B6IZ99
Organism   Streptococcus pyogenes strain emm22.8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 107852..118790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT61_RS00720 (ETT61_00720) rpoC 108600..112241 (+) 3642 WP_136020263.1 DNA-directed RNA polymerase subunit beta' -
  ETT61_RS00725 (ETT61_00725) - 112394..112759 (+) 366 WP_002986560.1 DUF1033 family protein -
  ETT61_RS00730 (ETT61_00730) comYA 112852..113790 (+) 939 WP_023079010.1 competence type IV pilus ATPase ComGA Machinery gene
  ETT61_RS00735 (ETT61_00735) comYB 113726..114760 (+) 1035 WP_231871599.1 competence type IV pilus assembly protein ComGB Machinery gene
  ETT61_RS00740 (ETT61_00740) comYC 114762..115088 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  ETT61_RS00745 (ETT61_00745) comGD 115063..115490 (+) 428 Protein_98 competence type IV pilus minor pilin ComGD -
  ETT61_RS00750 (ETT61_00750) comGE 115447..115731 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  ETT61_RS00755 (ETT61_00755) comYF 115724..116158 (+) 435 WP_136020264.1 competence type IV pilus minor pilin ComGF Machinery gene
  ETT61_RS00760 (ETT61_00760) comGG 116142..116468 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  ETT61_RS00765 (ETT61_00765) comYH 116566..117519 (+) 954 WP_136020265.1 class I SAM-dependent methyltransferase Machinery gene
  ETT61_RS00770 (ETT61_00770) - 117578..118774 (+) 1197 WP_010921803.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35086.41 Da        Isoelectric Point: 9.1037

>NTDB_id=341565 ETT61_RS00730 WP_023079010.1 112852..113790(+) (comYA) [Streptococcus pyogenes strain emm22.8]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQKA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YVRLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=341565 ETT61_RS00730 WP_023079010.1 112852..113790(+) (comYA) [Streptococcus pyogenes strain emm22.8]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAAGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAAAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGTAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B6IZ99

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

61.415

99.679

0.612
  comYA Streptococcus mutans UA159

61.415

99.679

0.612
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.679

0.606
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.359

0.599
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

99.679

0.587
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

39.31

92.949

0.365

Multiple sequence alignment