Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   ETT64_RS00770 Genome accession   NZ_CP035435
Coordinates   116428..117462 (+) Length   344 a.a.
NCBI ID   WP_227868957.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm64.3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 111428..122462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT64_RS00760 (ETT64_00760) - 115096..115461 (+) 366 WP_002986560.1 DUF1033 family protein -
  ETT64_RS00765 (ETT64_00765) comYA 115554..116492 (+) 939 WP_063812149.1 competence type IV pilus ATPase ComGA Machinery gene
  ETT64_RS00770 (ETT64_00770) comYB 116428..117462 (+) 1035 WP_227868957.1 competence type IV pilus assembly protein ComGB Machinery gene
  ETT64_RS00775 (ETT64_00775) comYC 117464..117790 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  ETT64_RS00780 (ETT64_00780) comGD 117765..118193 (+) 429 WP_136293154.1 competence type IV pilus minor pilin ComGD -
  ETT64_RS00785 (ETT64_00785) comGE 118150..118434 (+) 285 WP_315940846.1 competence type IV pilus minor pilin ComGE -
  ETT64_RS00790 (ETT64_00790) comYF 118427..118861 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  ETT64_RS00795 (ETT64_00795) comGG 118845..119171 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  ETT64_RS00800 (ETT64_00800) comYH 119269..120222 (+) 954 WP_136293153.1 class I SAM-dependent methyltransferase Machinery gene
  ETT64_RS00805 (ETT64_00805) - 120281..121477 (+) 1197 WP_011284424.1 acetate kinase -
  ETT64_RS00810 (ETT64_00810) - 121664..121972 (+) 309 Protein_111 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39690.18 Da        Isoelectric Point: 9.7085

>NTDB_id=341409 ETT64_RS00770 WP_227868957.1 116428..117462(+) (comYB) [Streptococcus pyogenes strain emm64.3]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEIITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=341409 ETT64_RS00770 WP_227868957.1 116428..117462(+) (comYB) [Streptococcus pyogenes strain emm64.3]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAATTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.687

98.547

0.529
  comYB Streptococcus mutans UA159

53.687

98.547

0.529
  comYB Streptococcus gordonii str. Challis substr. CH1

50.294

98.837

0.497
  comGB/cglB Streptococcus mitis NCTC 12261

47.321

97.674

0.462
  comGB/cglB Streptococcus mitis SK321

47.447

96.802

0.459
  comGB/cglB Streptococcus pneumoniae Rx1

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae D39

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae R6

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae TIGR4

45.345

96.802

0.439
  comGB Lactococcus lactis subsp. cremoris KW2

42.462

94.477

0.401

Multiple sequence alignment