Detailed information NTG0334105

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EP550_RS01560
• Genome accession: NZ_CP035043
• Coordinates: 329517..330575 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain ABUH796
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 324517..335575

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EP550_RS01545 (EP550_01545)	-	325622..325954 (+)	333	WP_000169594.1	hypothetical protein	-
EP550_RS01550 (EP550_01550)	-	325957..326781 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
EP550_RS01555 (EP550_01555)	ponA	326799..329354 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
EP550_RS01560 (EP550_01560)	pilM	329517..330575 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
EP550_RS01565 (EP550_01565)	pilN	330575..331216 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
EP550_RS01570 (EP550_01570)	pilO	331213..331953 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
EP550_RS01575 (EP550_01575)	pilP	331964..332491 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
EP550_RS01580 (EP550_01580)	pilQ	332559..334724 (+)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
EP550_RS01585 (EP550_01585)	aroK	334736..335305 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=334105 EP550_RS01560 WP_002014066.1 329517..330575(+) (pilM) [Acinetobacter baumannii strain ABUH796]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=334105 EP550_RS01560 WP_002014066.1 329517..330575(+) (pilM) [Acinetobacter baumannii strain ABUH796]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423