Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   EMO93_RS01935 Genome accession   NZ_CP035003
Coordinates   422603..423100 (-) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain PCFA-221     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417603..428100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EMO93_RS01910 gcvPB 417765..419237 (-) 1473 WP_000202179.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  EMO93_RS01915 gcvPA 419230..420576 (-) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  EMO93_RS01920 gcvT 420596..421687 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  EMO93_RS01925 - 421846..422370 (-) 525 WP_001015120.1 shikimate kinase -
  EMO93_RS01930 - 422360..422506 (-) 147 WP_001789879.1 hypothetical protein -
  EMO93_RS01935 comGF 422603..423100 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  EMO93_RS01940 comGE 423018..423317 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  EMO93_RS01945 comGD 423304..423750 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  EMO93_RS01950 comGC 423728..423994 (-) 267 WP_000927911.1 competence type IV pilus major pilin ComGC Machinery gene
  EMO93_RS01955 comGB 424052..425122 (-) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  EMO93_RS01960 comGA 425094..426068 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  EMO93_RS01965 - 426120..426743 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  EMO93_RS01970 - 426740..427069 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EMO93_RS01975 - 427069..428055 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=333873 EMO93_RS01935 WP_001825340.1 422603..423100(-) (comGF) [Staphylococcus aureus strain PCFA-221]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=333873 EMO93_RS01935 WP_001825340.1 422603..423100(-) (comGF) [Staphylococcus aureus strain PCFA-221]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97

Multiple sequence alignment