Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EO946_RS13025 Genome accession   NZ_CP034943
Coordinates   2525869..2526279 (-) Length   136 a.a.
NCBI ID   WP_003226105.1    Uniprot ID   E0U4U4
Organism   Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2520869..2531279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO946_RS12990 (EO946_12990) - 2520899..2521039 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  EO946_RS12995 (EO946_12995) - 2521342..2522073 (-) 732 WP_003226118.1 SGNH/GDSL hydrolase family protein -
  EO946_RS13005 (EO946_13005) - 2522324..2523076 (-) 753 WP_003226114.1 N-acetylmuramoyl-L-alanine amidase -
  EO946_RS13010 (EO946_13010) - 2523271..2523897 (+) 627 WP_003226112.1 TVP38/TMEM64 family protein -
  EO946_RS13015 (EO946_13015) gnd 2523916..2524809 (-) 894 WP_003226110.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  EO946_RS13020 (EO946_13020) - 2525111..2525836 (+) 726 WP_032727305.1 hypothetical protein -
  EO946_RS13025 (EO946_13025) nucA/comI 2525869..2526279 (-) 411 WP_003226105.1 sporulation-specific Dnase NucB Machinery gene
  EO946_RS13030 (EO946_13030) sigK 2526475..2527203 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  EO946_RS13035 (EO946_13035) - 2527360..2527551 (-) 192 Protein_2508 recombinase -
  EO946_RS21045 (EO946_13040) - 2527509..2527645 (-) 137 Protein_2509 hypothetical protein -
  EO946_RS13045 (EO946_13045) fumC 2527811..2529199 (-) 1389 WP_003226090.1 class II fumarate hydratase -
  EO946_RS13050 (EO946_13050) - 2529354..2530244 (+) 891 WP_003226089.1 LysR family transcriptional regulator -
  EO946_RS13055 (EO946_13055) - 2530676..2530840 (-) 165 Protein_2512 recombinase family protein -
  EO946_RS21050 (EO946_13060) - 2530798..2530934 (-) 137 Protein_2513 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14953.00 Da        Isoelectric Point: 5.1750

>NTDB_id=333596 EO946_RS13025 WP_003226105.1 2525869..2526279(-) (nucA/comI) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
MKKWMAGLFLAAAVLLCLMVPQQIQGASLYDKVLYFPLSRYPETGDHIKDAIAEGHSDICTIDRDGADKRRQESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=333596 EO946_RS13025 WP_003226105.1 2525869..2526279(-) (nucA/comI) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
TTCTTTGTATGACAAAGTGTTGTATTTTCCGCTGTCTCGTTATCCGGAAACGGGGGATCATATTAAAGATGCGATTGCAG
AGGGACATTCTGATATTTGCACTATCGACAGGGATGGAGCGGACAAAAGGCGGCAGGAATCATTAAAAGGAATTCCGACG
AAGCCAGGCTATGACCGGGATGAGTGGCCGATGGCGGTTTGTGAGGAAGGCGGCGCAGGAGCTGATGTCCGATATGTCAC
GCCTTCTGATAATCGCGGGGCCGGCTCGTGGGTAGGGAATCAAATGAGCGGTTATCCTGACGGCACCAGAGTGCTCTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0U4U4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.478

84.559

0.537

Multiple sequence alignment