Detailed information NTG0333595

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EO946_RS12940
• Genome accession: NZ_CP034943
• Coordinates: 2513938..2514555 (-)
• Length: 205 a.a.
• NCBI ID: WP_003226145.1
• Uniprot ID: -
• Organism: Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2508938..2519555

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EO946_RS12910 (EO946_12910)	rpsT	2509239..2509505 (+)	267	WP_003226155.1	30S ribosomal protein S20	-
EO946_RS12915 (EO946_12915)	holA	2509520..2510563 (-)	1044	WP_003226154.1	DNA polymerase III subunit delta	-
EO946_RS12920 (EO946_12920)	-	2510603..2510752 (-)	150	WP_136975859.1	hypothetical protein	-
EO946_RS12925 (EO946_12925)	-	2510793..2510927 (+)	135	WP_003226152.1	YqzM family protein	-
EO946_RS12930 (EO946_12930)	comEC	2510968..2513298 (-)	2331	WP_003226149.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EO946_RS12935 (EO946_12935)	-	2513302..2513871 (-)	570	WP_003226147.1	ComE operon protein 2	-
EO946_RS12940 (EO946_12940)	comEA	2513938..2514555 (-)	618	WP_003226145.1	helix-hairpin-helix domain-containing protein	Machinery gene
EO946_RS12945 (EO946_12945)	comER	2514639..2515460 (+)	822	WP_003226143.1	late competence protein ComER	-
EO946_RS12950 (EO946_12950)	-	2515526..2516269 (-)	744	WP_003226141.1	class I SAM-dependent methyltransferase	-
EO946_RS12955 (EO946_12955)	rsfS	2516266..2516622 (-)	357	WP_003226139.1	ribosome silencing factor	-
EO946_RS12960 (EO946_12960)	yqeK	2516640..2517200 (-)	561	WP_003226137.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EO946_RS12965 (EO946_12965)	-	2517190..2517759 (-)	570	WP_003226135.1	nicotinate-nucleotide adenylyltransferase	-
EO946_RS12970 (EO946_12970)	yhbY	2517771..2518061 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
EO946_RS12975 (EO946_12975)	aroE	2518055..2518897 (-)	843	WP_003226130.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21919.78 Da
• Isoelectric Point: 5.1470

>NTDB_id=333595 EO946_RS12940 WP_003226145.1 2513938..2514555(-) (comEA) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
MNWLNHHKKAIILGASAAVFIAIIFFLASGKNKESMKQTVSTETENTEVKQEAVKDESNETIVIDIKGAVKHPGVYEMRT
GDRLSQAIEKAGGTSEQADEMQVNLAGLLQDGTVVYIPKRGEETAVQQGVGGAIQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=333595 EO946_RS12940 WP_003226145.1 2513938..2514555(-) (comEA) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
ATGAATTGGTTGAATCATCATAAGAAAGCAATTATTTTAGGGGCCTCTGCGGCTGTTTTCATAGCGATCATTTTCTTCCT
GGCCAGCGGGAAAAACAAAGAGTCGATGAAGCAGACTGTATCAACTGAGACAGAAAATACGGAGGTAAAGCAGGAAGCAG
TCAAAGATGAGTCAAACGAAACGATTGTGATTGACATCAAAGGTGCTGTTAAGCACCCTGGTGTTTATGAAATGCGAACA
GGGGACAGATTATCCCAGGCAATTGAGAAAGCCGGCGGGACCAGTGAACAAGCAGACGAAATGCAAGTGAATTTGGCGGG
GCTGCTGCAGGACGGGACAGTGGTGTACATCCCGAAGAGGGGTGAGGAGACAGCGGTGCAGCAAGGAGTCGGAGGCGCCA
TCCAAAGCGATGGAGGAAAGGGGGCGCTGGTAAATATCAATACAGCAACCTTAGAGGAATTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAATGGGCGTTTCCAGACAATTGAAGATATTACGAAGGT
TTCAGGGATCGGTGAAAAGTCGTTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	89.268	100	0.893
comEA	Latilactobacillus sakei subsp. sakei 23K	31.646	100	0.366
comEA	Staphylococcus aureus MW2	37.688	97.073	0.366