Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   FGL73_RS08555 Genome accession   NZ_CP042408
Coordinates   1779173..1780174 (-) Length   333 a.a.
NCBI ID   WP_003131994.1    Uniprot ID   Q9CDL3
Organism   Lactococcus lactis strain CBA3619     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1774173..1785174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL73_RS08510 (FGL73_08510) - 1774245..1774610 (-) 366 WP_003131985.1 MerR family transcriptional regulator -
  FGL73_RS08515 (FGL73_08515) - 1774711..1775148 (-) 438 WP_038602480.1 low molecular weight protein-tyrosine-phosphatase -
  FGL73_RS12605 - 1775292..1775456 (-) 165 WP_186758662.1 hypothetical protein -
  FGL73_RS12845 - 1775748..1775915 (-) 168 WP_230315131.1 hypothetical protein -
  FGL73_RS08525 (FGL73_08525) - 1776197..1776499 (+) 303 Protein_1690 integrase core domain-containing protein -
  FGL73_RS08530 (FGL73_08530) - 1776743..1777027 (-) 285 WP_038602483.1 bacteriocin immunity protein -
  FGL73_RS08535 (FGL73_08535) - 1777065..1777238 (-) 174 WP_229049640.1 class IIb bacteriocin, lactobin A/cerein 7B family -
  FGL73_RS08540 (FGL73_08540) - 1777350..1777670 (-) 321 WP_038602485.1 hypothetical protein -
  FGL73_RS08545 (FGL73_08545) - 1777805..1777966 (-) 162 WP_118263635.1 bacteriocin -
  FGL73_RS08550 (FGL73_08550) - 1778152..1779036 (-) 885 WP_038602487.1 XRE/MutR family transcriptional regulator -
  FGL73_RS08555 (FGL73_08555) ruvB 1779173..1780174 (-) 1002 WP_003131994.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  FGL73_RS08560 (FGL73_08560) ruvA 1780301..1780894 (-) 594 WP_003131995.1 Holliday junction branch migration protein RuvA Machinery gene
  FGL73_RS08565 (FGL73_08565) hexB 1781006..1782976 (-) 1971 WP_146978291.1 DNA mismatch repair endonuclease MutL Machinery gene
  FGL73_RS08570 (FGL73_08570) - 1783105..1783800 (-) 696 WP_146978292.1 hypothetical protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 37633.17 Da        Isoelectric Point: 4.5825

>NTDB_id=331616 FGL73_RS08555 WP_003131994.1 1779173..1780174(-) (ruvB) [Lactococcus lactis strain CBA3619]
MNDILNKEPMEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNI
KQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTR
AGMLSNPLRARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVD
KAITDKALSILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQR
AYEHLGYVYNEED

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=331616 FGL73_RS08555 WP_003131994.1 1779173..1780174(-) (ruvB) [Lactococcus lactis strain CBA3619]
ATGAACGATATATTAAACAAAGAGCCAATGGAAGAAGATTACGGTATTGAAAAATCTCTACGTCCGCAATTTTTTAATCA
ATATATTGGTCAAGATAAAGTCAAAGAACAATTAGAAATCTTTATTAAAGCAGCAAAAATGCGTGAAGAAGTTTTGGATC
ACGTATTACTTTTTGGTCCTCCGGGACTTGGTAAGACAACAATGGCATTTGTCATTGCCAATGAGTTGGGGGTTAATATT
AAACAAACAGCGGGTCCTGCCATTGAAAAGCCAGGAGATTTAGTTGCGATTTTGAATGAATTGGAACCTGGCGATGTGCT
ATTTATTGATGAAATTCATCGGATGCCAATGCAAGTTGAAGAAGTTCTTTATAGCGCTATGGAAGATTTCTACATTGATA
TTATGCTTGGTTCTGGTGATGGTTCACGCTCGGTTCATTTAGATTTGCCGCCATTTACTCTAGTTGGGGCAACAACAAGA
GCTGGAATGCTTTCGAATCCATTGCGTGCCCGTTTTGGAATTTCAAGTCATATGGAGTATTATCAAGAGCGTGATTTGGA
AGAAATTGTTAAAAGAACAGCTGATATTTTTGAAGTCGAAGTTATTGATAATGCCGCTTTAGAGATTGCTTTACGTAGTC
GAGGGACTCCGCGGATTGCCAATCGTTTACTTAAAAGAGTGCGTGATTTTGCTCAAATAATGGGGGATGGCCGAGTGGAT
AAAGCAATTACCGATAAGGCCTTGTCTATTCTTGATGTTGATGCCGCTGGTTTGGACTATATTGACCAAAAAATTCTCCG
CACAATGATTGAAATGTACCACGGTGGTCCTGTTGGGATTGGAACTTTGGCGGTAAATATTGCTGAAGACCGTGAAACGG
TCGAGGATATGTATGAACCTTATTTGATTCAAAAAGGATTTTTGATGAGAACCAAACAGGGACGTAAGGTGACTCAAAGA
GCCTATGAACACCTAGGTTACGTTTATAATGAAGAAGATTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

RuvB AAA lid domain (AAA_lid_4)

(181-254)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CDL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae R6

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae D39

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae TIGR4

78.313

99.7

0.781
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.491

96.697

0.595
  ruvB Helicobacter pylori 26695

51.242

96.697

0.495
  ruvB Synechocystis sp. PCC 6803

51.613

93.093

0.48