Detailed information NTG0033143

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: SPJ_RS03705
• Genome accession: NC_012466
• Coordinates: 712769..713443 (+)
• Length: 224 a.a.
• NCBI ID: WP_000590640.1
• Uniprot ID: D4I4V1
• Organism: Streptococcus pneumoniae JJA
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	711295..712551	712769..713443	flank	218

Gene organization within MGE regions

Location: 711295..713443

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPJ_RS03700 (SPJ_0743)	-	711295..712551 (-)	1257	WP_000436237.1	ISL3-like element IS1167A family transposase	-
SPJ_RS03705 (SPJ_0744)	ciaR	712769..713443 (+)	675	WP_000590640.1	two-component system response regulator CiaR	Regulator

Sequence

Protein

• Length: 224 a.a.
• Molecular weight: 25466.29 Da
• Isoelectric Point: 4.3283

>NTDB_id=33143 SPJ_RS03705 WP_000590640.1 712769..713443(+) (ciaR) [Streptococcus pneumoniae JJA]
MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTA
KESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGYLLKDVQ

Nucleotide

• Length: 675 bp

>NTDB_id=33143 SPJ_RS03705 WP_000590640.1 712769..713443(+) (ciaR) [Streptococcus pneumoniae JJA]
ATGATAAAAATCTTATTGGTTGAGGATGACCTAGGTCTGTCAAATTCAGTATTTGACTTTTTAGACGATTTTGCGGATGT
TATGCAGGTATTTGATGGAGAAGAAGGTCTCTACGAAGCTGAGAGTGGTGTCTATGACTTGATTTTGCTGGATTTGATGT
TGCCAGAAAAAAATGGTTTCCAAGTCTTAAAAGAATTGCGTGAAAAGGGAATTACGACACCAGTTCTGATTATGACTGCC
AAGGAAAGTTTGGATGACAAGGGACATGGATTTGAACTGGGAGCGGATGATTATCTGACCAAACCTTTCTACCTAGAAGA
ACTTAAAATGCGAATTCAGGCCCTTCTCAAACGTTCAGGGAAGTTTAATGAAAACACCTTGACTTATGGGAATATCGTGG
TTAATTTATCAACCAATACCGTTAAAGTTGAAGATACTCCTGTCGAATTGCTGGGGAAAGAGTTCGATTTACTAGTTTAT
TTCCTTCAAAATCAAAATGTGATTTTGCCTAAGACGCAGATTTTTGACCGTCTATGGGGATTTGATAGTGATACAACGAT
TTCGGTTGTCGAAGTCTATGTTTCAAAAGTCCGTAAGAAATTAAAGGGAACCACTTTTGCAGAGAATTTGCAAACCTTGC
GTAGTGTTGGGTATCTTTTAAAAGATGTTCAGTAA

Domains

Predicted by InterproScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (146-220)

• Response regulator receiver domain (Response_reg) (4-112)

3D structure

• AlphaFold DB: D4I4V1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	100	100	1
ciaR	Streptococcus pneumoniae D39	100	100	1
ciaR	Streptococcus pneumoniae R6	100	100	1
ciaR	Streptococcus pneumoniae TIGR4	100	100	1
ciaR	Streptococcus mutans UA159	88.393	100	0.884
covR	Lactococcus lactis subsp. lactis strain DGCC12653	36.726	100	0.371
vicR	Streptococcus mutans UA159	35.622	100	0.371