Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EI420_RS10060 Genome accession   NZ_CP034367
Coordinates   2108906..2110618 (+) Length   570 a.a.
NCBI ID   WP_240645179.1    Uniprot ID   -
Organism   Vreelandella venusta strain MA-ZP17-13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2103906..2115618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EI420_RS10030 (EI420_10030) - 2105195..2105554 (+) 360 WP_125748102.1 hypothetical protein -
  EI420_RS10035 (EI420_10035) - 2105633..2106385 (+) 753 WP_125748105.1 VIT1/CCC1 transporter family protein -
  EI420_RS10045 (EI420_10045) - 2106735..2107544 (-) 810 WP_125748108.1 inositol monophosphatase family protein -
  EI420_RS10050 (EI420_10050) - 2107635..2108090 (-) 456 WP_125748112.1 anti-virulence regulator CigR family protein -
  EI420_RS20830 (EI420_10055) - 2108255..2108647 (-) 393 WP_167468857.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EI420_RS10060 (EI420_10060) pilB 2108906..2110618 (+) 1713 WP_240645179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EI420_RS10065 (EI420_10065) - 2110625..2111854 (+) 1230 WP_125748115.1 type II secretion system F family protein -
  EI420_RS10070 (EI420_10070) pilD 2111909..2112754 (+) 846 WP_125748118.1 A24 family peptidase Machinery gene
  EI420_RS10075 (EI420_10075) coaE 2112795..2113391 (+) 597 WP_125748121.1 dephospho-CoA kinase -
  EI420_RS10080 (EI420_10080) yacG 2113431..2113664 (+) 234 WP_125748124.1 DNA gyrase inhibitor YacG -
  EI420_RS10085 (EI420_10085) - 2113678..2114550 (+) 873 WP_125748127.1 DNA replication terminus site-binding protein -
  EI420_RS10090 (EI420_10090) - 2114582..2114923 (-) 342 WP_125748130.1 hypothetical protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62496.45 Da        Isoelectric Point: 5.5034

>NTDB_id=330985 EI420_RS10060 WP_240645179.1 2108906..2110618(+) (pilB) [Vreelandella venusta strain MA-ZP17-13]
MHSGLHGIAKRLVENGELTDAQATVAENTATELDVSLLQHLVDSGLVESGAAAQAAAWEFGLPVVELDVIRINALPPAND
YPVNLLERLEVLPLARHGHRLTVAVPYPATLTQLDELQFATGLSIDGVLAPVDQLKSTLTQYLAQNERSMLDELDNVDDA
ISALNIVQQDEDEATLDKATKESEDAPVVKFVNKILLDAINRGASDIHFEPYETLYRVRFRIDGMLFEIARPPFALRHRI
AARVKIMSRLDISERRLPQDGAIKLQLSRTHSIDFRVNSLPTVFGEKIVLRLLDPATAQLSIEQLGFSSEQQAVYEHALK
KPQGMILVTGPTGSGKTVTLYTGINILNQIERNICTAEDPVEIKVSGVNQVNVLPKIGLNFASALRAFLRQDPDVVMVGE
IRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGIASYNIASSVSLIIAQRLARKLCSLCKTPAQIPDEALRQA
GFSQQDIHNGTIYSAVGCKHCTQGYKGRIGIYEVVPVTDAMRQLIMRDVDALNIDQQARREGYPSLYQRCLSRVLDGSTS
LAEVNRISKE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=330985 EI420_RS10060 WP_240645179.1 2108906..2110618(+) (pilB) [Vreelandella venusta strain MA-ZP17-13]
ATGCATAGCGGTCTGCACGGTATCGCTAAACGGCTGGTTGAAAATGGCGAGCTGACCGATGCTCAGGCGACTGTCGCGGA
AAACACAGCAACAGAGCTTGATGTGTCGTTGTTACAGCACCTAGTTGATAGCGGGCTAGTAGAATCAGGCGCAGCAGCGC
AGGCCGCTGCCTGGGAGTTTGGCTTGCCGGTCGTTGAGCTAGATGTCATTCGTATAAATGCGCTGCCACCCGCCAACGAC
TATCCCGTTAACTTATTAGAGCGTCTAGAGGTCCTTCCCCTGGCACGCCATGGGCATCGACTGACGGTAGCTGTACCCTA
CCCGGCAACGCTAACCCAGCTAGATGAGCTGCAATTCGCTACCGGGCTAAGTATCGATGGAGTACTGGCTCCCGTTGATC
AACTTAAAAGCACTCTAACGCAGTATCTAGCGCAAAATGAGCGCAGCATGCTAGATGAATTGGACAACGTAGATGATGCG
ATCAGCGCGTTGAATATTGTTCAGCAGGACGAAGATGAGGCAACCTTAGACAAGGCAACAAAAGAGAGTGAAGATGCCCC
TGTCGTCAAATTCGTCAATAAAATACTCTTAGATGCTATCAACCGCGGCGCTTCCGATATTCACTTTGAGCCTTATGAAA
CCCTGTACCGGGTGCGCTTTCGTATTGATGGCATGCTGTTTGAAATTGCCCGCCCACCTTTTGCGCTTCGGCACCGCATT
GCGGCTCGGGTAAAAATCATGTCTCGGCTGGACATCTCTGAGCGGCGCCTACCCCAGGATGGGGCAATCAAGCTGCAGCT
TTCACGCACTCACTCAATTGATTTTCGAGTGAACTCGCTGCCCACTGTGTTTGGTGAAAAAATAGTGCTGCGCTTGCTAG
ATCCTGCCACTGCTCAACTCTCTATCGAACAACTAGGCTTTAGCTCTGAACAACAGGCCGTTTATGAACACGCGCTTAAG
AAGCCTCAAGGAATGATTCTGGTTACCGGGCCTACGGGAAGCGGTAAAACAGTGACGCTTTATACAGGCATCAACATTCT
TAACCAAATTGAGCGTAATATTTGCACCGCCGAAGACCCCGTAGAAATTAAGGTGTCGGGGGTCAATCAGGTCAACGTTC
TTCCTAAAATAGGTCTGAACTTCGCTAGCGCTCTACGTGCCTTTTTACGCCAAGATCCCGATGTCGTCATGGTGGGCGAA
ATTCGTGATTTGGAAACTGCCGAAATTGCCGTGAAAGCAGCCCAAACGGGTCACTTAGTGCTATCTACGGTACACACTAA
CTCCGCCGCTGAAACACTAACCCGCTTAGCAAATATGGGCATCGCTTCCTATAACATCGCCAGCTCGGTTAGCTTGATCA
TTGCCCAACGTTTGGCACGCAAGCTATGCAGCCTATGCAAAACCCCCGCACAGATTCCTGACGAAGCACTGCGTCAGGCT
GGCTTTAGCCAGCAGGACATTCACAACGGCACGATTTATTCAGCCGTCGGCTGTAAACACTGCACCCAGGGCTATAAAGG
TCGTATTGGTATCTATGAAGTGGTGCCTGTTACTGATGCCATGCGCCAGTTGATTATGCGTGATGTTGACGCGCTGAATA
TCGATCAGCAAGCACGCCGCGAAGGTTACCCTAGCCTTTATCAACGCTGCTTAAGTCGTGTGCTGGATGGCAGCACTAGC
TTGGCAGAAGTTAACCGCATCAGTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-147)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.327

99.123

0.509
  pilB Acinetobacter baumannii D1279779

49.824

99.649

0.496
  pilB Acinetobacter baylyi ADP1

49.474

100

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

48.427

100

0.486
  pilB Vibrio cholerae strain A1552

49.005

97.018

0.475
  pilB Vibrio campbellii strain DS40M4

49.257

94.386

0.465
  pilF Neisseria gonorrhoeae MS11

47.917

84.211

0.404

Multiple sequence alignment