Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   FP509_RS01460 Genome accession   NZ_CP041999
Coordinates   233017..233478 (+) Length   153 a.a.
NCBI ID   WP_001878820.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain GBS11     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 228017..238478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FP509_RS01415 (FP509_01410) - 228094..228348 (+) 255 WP_000611524.1 phage holin -
  FP509_RS01420 (FP509_01415) - 228474..229805 (+) 1332 WP_000257237.1 GH25 family lysozyme -
  FP509_RS01425 (FP509_01420) - 229940..230836 (+) 897 WP_001061853.1 sensor histidine kinase -
  FP509_RS01430 (FP509_01425) - 230830..231129 (+) 300 WP_000258211.1 STAS-like domain-containing protein -
  FP509_RS01435 (FP509_01430) - 231239..231439 (+) 201 WP_000076712.1 CsbD family protein -
  FP509_RS01440 (FP509_01435) - 231480..231650 (-) 171 WP_000356856.1 hypothetical protein -
  FP509_RS10940 - 231786..231911 (-) 126 WP_017285425.1 hypothetical protein -
  FP509_RS01445 (FP509_01440) comGC 232135..232404 (+) 270 WP_000962019.1 competence type IV pilus major pilin ComGC Machinery gene
  FP509_RS01450 (FP509_01445) comGD 232379..232792 (+) 414 WP_000244261.1 competence type IV pilus minor pilin ComGD Machinery gene
  FP509_RS01455 (FP509_01450) comGE 232764..233063 (+) 300 WP_000234804.1 competence type IV pilus minor pilin ComGE Machinery gene
  FP509_RS01460 (FP509_01455) comGF 233017..233478 (+) 462 WP_001878820.1 competence type IV pilus minor pilin ComGF Machinery gene
  FP509_RS01465 (FP509_01460) comGG 233456..233827 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG Machinery gene
  FP509_RS01470 (FP509_01465) comGH 233942..234916 (+) 975 WP_001008574.1 class I SAM-dependent methyltransferase Machinery gene
  FP509_RS01475 (FP509_01470) - 234948..236141 (+) 1194 WP_000047534.1 acetate kinase -
  FP509_RS01480 (FP509_01475) - 236293..236499 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  FP509_RS01485 (FP509_01480) - 236558..236695 (+) 138 WP_001865900.1 hypothetical protein -
  FP509_RS01490 (FP509_01485) - 236736..237191 (+) 456 WP_000905673.1 hypothetical protein -
  FP509_RS01495 (FP509_01490) - 237260..237925 (+) 666 WP_000008113.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17874.95 Da        Isoelectric Point: 10.0299

>NTDB_id=329475 FP509_RS01460 WP_001878820.1 233017..233478(+) (comGF) [Streptococcus agalactiae strain GBS11]
MKIIRKFCTYQKLRVKAFTLLKCLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQ
NKLYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide


Download         Length: 462 bp        

>NTDB_id=329475 FP509_RS01460 WP_001878820.1 233017..233478(+) (comGF) [Streptococcus agalactiae strain GBS11]
TTGAAGATAATAAGGAAATTTTGCACGTATCAAAAGTTAAGGGTTAAAGCATTTACGCTTTTAAAATGTTTGGTAGCATT
GGTTACAATCACAGGAGCTTTACTAGTTTATCAAGGACTGACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTT
CCAGTCAGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAG
AACAAACTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTA
TGATGGTCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTG
TTTTTTATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(49-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA159

47.183

92.81

0.438
  comGF Streptococcus mutans UA140

47.183

92.81

0.438
  comGF/cglF Streptococcus pneumoniae Rx1

42.446

90.85

0.386
  comGF/cglF Streptococcus pneumoniae D39

42.446

90.85

0.386
  comGF/cglF Streptococcus pneumoniae R6

42.446

90.85

0.386
  comGF/cglF Streptococcus pneumoniae TIGR4

42.446

90.85

0.386
  comGF/cglF Streptococcus mitis SK321

43.284

87.582

0.379
  comGF/cglF Streptococcus mitis NCTC 12261

42.537

87.582

0.373
  comGF Lactococcus lactis subsp. cremoris KW2

40

91.503

0.366

Multiple sequence alignment