Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   FO444_RS10295 Genome accession   NZ_CP041759
Coordinates   2042930..2044813 (-) Length   627 a.a.
NCBI ID   WP_211752841.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain IMAU11823     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 2037930..2049813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FO444_RS10265 (FO444_10235) - 2038332..2039012 (-) 681 WP_017864968.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  FO444_RS10270 (FO444_10240) macP 2039044..2039322 (-) 279 WP_003130796.1 cell wall synthase accessory phosphoprotein MacP -
  FO444_RS10275 (FO444_10245) - 2039315..2039899 (-) 585 WP_017864969.1 NUDIX hydrolase -
  FO444_RS10280 (FO444_10250) glmU 2040004..2041380 (-) 1377 WP_017864970.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  FO444_RS10285 (FO444_10255) proC 2041608..2042396 (+) 789 WP_012898440.1 pyrroline-5-carboxylate reductase -
  FO444_RS10290 (FO444_10260) rpsO 2042475..2042744 (-) 270 WP_015427039.1 30S ribosomal protein S15 -
  FO444_RS10295 (FO444_10265) pknB 2042930..2044813 (-) 1884 WP_211752841.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  FO444_RS10300 (FO444_10270) - 2044813..2045589 (-) 777 WP_003130802.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  FO444_RS10305 (FO444_10275) rsmB 2045669..2046943 (-) 1275 WP_017864972.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  FO444_RS10315 (FO444_10290) - 2048113..2048892 (-) 780 Protein_2023 peptidoglycan amidohydrolase family protein -
  FO444_RS10320 (FO444_10295) - 2048892..2049179 (-) 288 WP_058206378.1 phage holin -
  FO444_RS10325 (FO444_10300) - 2049193..2049417 (-) 225 WP_010905920.1 hemolysin XhlA family protein -
  FO444_RS10330 (FO444_10305) - 2049430..2049666 (-) 237 WP_211753286.1 hypothetical protein -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 67811.89 Da        Isoelectric Point: 4.7783

>NTDB_id=328338 FO444_RS10295 WP_211752841.1 2042930..2044813(-) (pknB) [Lactococcus lactis subsp. lactis strain IMAU11823]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPEQARGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKDHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVGNKNKKSKKGLIIGLIILLLVVGGATLAWVVSTPTNVKIPNVTNSTLSQAKSKIKDAKLKVGTVHKQQSSTI
AEGKVIKTDPTSGATVRSNSSVDIYVSTGNEDIIKIKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKAAGFTNITLDPQATTNQQADGYVYSVYPNVGT
SVDPTQEIVVTYSVYTAPSSSSTTSESTTTSETSSSTTSSTSSSTTSQPSTDNNNSSKEGSITSSSS

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=328338 FO444_RS10295 WP_211752841.1 2042930..2044813(-) (pknB) [Lactococcus lactis subsp. lactis strain IMAU11823]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCAAGCGCGTGGTTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAGCTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATTAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGACCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGATCATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGGAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CACTTAGCCAAGCAAAAAGCAAAATTAAAGATGCAAAACTTAAAGTGGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAAAGTTATCAAGACTGATCCAACTAGTGGAGCAACCGTGAGATCTAATAGCTCCGTTGATATCTATGTTTC
TACGGGAAATGAAGATATCATCAAAATAAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCCTTAAAAGCTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAATCAACAAGCTGATGGTTATGTTTACTCAGTGTATCCAAATGTAGGGACA
TCTGTTGATCCAACTCAAGAAATTGTAGTCACTTATAGCGTTTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACTACGACGTCTGAAACTTCATCAAGTACAACTTCAAGTACTTCAAGCTCTACAACAAGTCAGCCGTCAACAGATAACA
ACAATAGCAGCAAAGAAGGTTCAATAACTTCATCAAGTAGTTAA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(365-428)

PASTA domain (PASTA)

(435-501)

PASTA domain (PASTA)

(506-573)

Protein kinase domain (Pkinase)

(12-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

49.081

86.762

0.426
  stkP/pknB Streptococcus salivarius strain HSISS4

51.252

82.775

0.424
  stkP Streptococcus pneumoniae TIGR4

48.024

80.702

0.388
  stkP Streptococcus pneumoniae D39

47.826

80.702

0.386
  stkP Streptococcus pneumoniae R6

47.826

80.702

0.386
  stkP Streptococcus pneumoniae Rx1

47.628

80.702

0.384

Multiple sequence alignment