Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EGH20_RS00655 Genome accession   NZ_CP034026
Coordinates   103215..105410 (-) Length   731 a.a.
NCBI ID   WP_050156267.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain FQ20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 98215..110410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGH20_RS00610 (EGH20_00610) - 98687..99529 (-) 843 WP_003687345.1 class II glutamine amidotransferase -
  EGH20_RS00625 (EGH20_00625) aroB 100271..101350 (-) 1080 WP_124723890.1 3-dehydroquinate synthase -
  EGH20_RS00635 (EGH20_00635) - 101430..101942 (-) 513 WP_003687349.1 shikimate kinase -
  EGH20_RS00655 (EGH20_00655) pilQ 103215..105410 (-) 2196 WP_050156267.1 type IV pilus secretin PilQ Machinery gene
  EGH20_RS00660 (EGH20_00660) pilP 105429..105974 (-) 546 WP_124723892.1 pilus assembly protein PilP Machinery gene
  EGH20_RS00665 (EGH20_00665) pilO 105992..106639 (-) 648 WP_003690570.1 type 4a pilus biogenesis protein PilO -
  EGH20_RS00670 (EGH20_00670) pilN 106640..107239 (-) 600 WP_003687354.1 PilN domain-containing protein Machinery gene
  EGH20_RS00675 (EGH20_00675) - 107242..108357 (-) 1116 WP_003687356.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 731 a.a.        Molecular weight: 78872.99 Da        Isoelectric Point: 10.0901

>NTDB_id=328267 EGH20_RS00655 WP_050156267.1 103215..105410(-) (pilQ) [Neisseria gonorrhoeae strain FQ20]
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTALQRSLD
VADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIR
TILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADL
GALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMI
EARIVEAADGFSRDLGVKFGATGRKKLKNETSAFGWGVNSGFRGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLEL
SASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDS
PAQCASGNNTILCISTKSLNTQAMVENGGTLIVGGIYEENNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPR
IIDTAGNSLRY

Nucleotide


Download         Length: 2196 bp        

>NTDB_id=328267 EGH20_RS00655 WP_050156267.1 103215..105410(-) (pilQ) [Neisseria gonorrhoeae strain FQ20]
ATGAATACCAAACTGACAAAAATCATTTCCGGTCTCTTTGTCGCAACCGCCGCCTTTCAGACGGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGATTGTCAACCCGA
CCGGCTTCGTAACCTCCTCACCGGCCCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGGATCAACAGGTACTC
GAATATGCCGATCCTCTGTTGAGCAAAATCAGTGCCGCACAAAACAGCAGCCGTGCGCGTCTGGTTCTGAATTTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGGAACAAAGTTTGGATATTCATTAACGAATCGGACGATACCGTGTCCGCCC
CCGCCCGCCCAGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTA
TCCGTATCCGCACCGTTCAGCCCGGCAAAACAACAGGCAGCGGCATCGGCAAAACAACAGGCGGCGACACCGGCAAAACA
AACCAATATCGATTTCCGCAAAGACGGCAAAAATGCCGGCATTATCGAATTGGCGGCATTGGGCTTTGCCGGGCAGCCCG
ACATCAGCCAACAGCACGACCACATCATCGTTACGCTGAAAAACCATACCCTGCCGACCGCGCTCCAACGCAGTTTGGAT
GTGGCAGACTTCAAAACACCGGTTCAAAAGGTTACGCTGAAACGCCTCAATAACGACACCCAGCTGATTATCACAACAAC
CGGCAACTGGGAACTCGTCAACAAATCCGCCGCGCCCGGATACTTTACCTTCCAAGTCCTGCCGAAAAAACAAAACCTCG
AGTCAGGCGGCGTGAACAATGCGCCCAAAACCTTCACAGGCCGGAAAATCTCCCTTGACTTCCAAGATGTCGAAATCCGC
ACCATCCTGCAGATTTTGGCAAAAGAATCCGGGATGAACATTGTTGCCAGCGACTCCGTCAACGGCAAAATGACCCTCTC
CCTCAAAGATGTGCCTTGGGATCAGGCTTTGGATTTGGTTATGCAGGCGCGCAACCTCGATATGCGCCAGCAAGGGAACA
TCGTCAACATCGCGCCCCGCGACGAGCTGCTTGCCAAAGACAAAGCCTTCTTACAGGCGGAAAAAGACATTGCCGATCTG
GGCGCGCTGTATTCCCAAAACTTCCAATTGAAATACAAAAATGTGGAAGAATTCCGCAGCATCCTGCGTTTGGACAATGC
CGACACGACCGGAAACCGCAACACGCTTGTCAGCGGCAGGGGCAGCGTGCTGATCGATCCCGCCACCAACACCCTGATTG
TTACCGATACCCGCAGCGTCATCGAAAAATTCCGCAAACTGATTGACGAATTGGACGTACCCGCGCAACAAGTGATGATT
GAGGCGCGTATCGTCGAAGCGGCAGACGGCTTCTCGCGCGATTTGGGCGTTAAGTTCGGCGCGACAGGCAGGAAAAAACT
GAAAAATGAGACGAGCGCATTCGGCTGGGGCGTGAACTCCGGCTTCAGGGGCGGCGATAAATGGGAGGCCCAAACCAAAA
TCAACCTGCCGGTTGCCGCTGCCGCAAACAGCATTTCGCTGGTGCGCGCGATTTCCTCCGGCGCGTTGAATTTGGAATTG
TCCGCATCCGAGTCGCTTTCAAAAACCAAAACGCTTGCCAATCCGCGCGTGCTGACCCAAAACCGCAAAGAGGCCAAAAT
CGAATCCGGTTACGAAATTCCTTTTACCGTAACTACAGCCTCGGGCGGCGGCAACTCTACCAACACGGAACTCAAAAAAG
CCGTCTTGGGGCTGACCGTTACGCCGAACATCACGCCCGACGGACAAATCATCATGACCGTCAAAATCAACAAAGACTCG
CCTGCACAATGTGCTTCAGGCAACAACACAATCCTATGTATTTCGACCAAAAGCCTGAATACGCAGGCTATGGTTGAAAA
CGGCGGCACTTTGATTGTCGGCGGTATTTATGAAGAAAACAACGGCAATACGCTGACCAAAGTCCCCCTGTTGGGCGACA
TCCCCGTTATCGGCAACCTCTTTAAAACACGCGGGAAAAAAACCGACCGCCGCGAACTGCTGATTTTCATTACCCCGAGG
ATTATAGATACGGCCGGCAACAGCCTGCGCTATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(406-477)

Bacterial type II and III secretion system protein (Secretin)

(563-723)

Secretin and TonB N terminus short domain (STN)

(333-378)

AMIN domain (AMIN)

(43-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

98.632

100

0.986
  pilQ Neisseria meningitidis MC58

90.897

100

0.956
  pilQ Neisseria meningitidis M1080

91.59

100

0.953

Multiple sequence alignment