Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EHF44_RS07830 Genome accession   NZ_CP033969
Coordinates   1332074..1333795 (-) Length   573 a.a.
NCBI ID   WP_124683221.1    Uniprot ID   A0A3G8GYP1
Organism   Cupriavidus pauculus strain FDAARGOS_614     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1327074..1338795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHF44_RS07805 (EHF44_07805) - 1327899..1328087 (-) 189 WP_124683216.1 DNA gyrase inhibitor YacG -
  EHF44_RS07810 (EHF44_07810) zapD 1328099..1328857 (-) 759 WP_124683217.1 cell division protein ZapD -
  EHF44_RS07815 (EHF44_07815) coaE 1329081..1329695 (-) 615 WP_124683218.1 dephospho-CoA kinase -
  EHF44_RS07820 (EHF44_07820) - 1329759..1330652 (-) 894 WP_124683219.1 A24 family peptidase -
  EHF44_RS07825 (EHF44_07825) - 1330680..1331945 (-) 1266 WP_124683220.1 type II secretion system F family protein -
  EHF44_RS07830 (EHF44_07830) pilF 1332074..1333795 (-) 1722 WP_124683221.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EHF44_RS07840 (EHF44_07840) ispB 1334307..1335236 (-) 930 WP_124685029.1 octaprenyl diphosphate synthase -
  EHF44_RS07845 (EHF44_07845) ltrA 1335400..1337100 (-) 1701 WP_124683222.1 group II intron reverse transcriptase/maturase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62963.68 Da        Isoelectric Point: 7.7727

>NTDB_id=327515 EHF44_RS07830 WP_124683221.1 1332074..1333795(-) (pilF) [Cupriavidus pauculus strain FDAARGOS_614]
MTLGLALAQSRRIAPALLAQLEQSAREKKTQLIDEIIGSGTMSAHDVAIFAADKYQLPLLDLSQYNLNRVPPALAGNREF
HAHRLLPLGRRENRVIVAMSDPANQAGVEAIRQKLNLPVETVVVEHDKLMKHVRAAGEALGTMKNISPVTPTAQKMIEYD
PSAAAAARKGPGNEIDDAPVVRFLQKLLTEAFNRGASDLHFEPFEFFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVLS
RLDISEKRVPQDGRMKLLIAVPKDKDGKETTDKAIDFRVSTLPTLFGEKIVMRILDSSTDKLDIDQLGYEPRQKELLMEV
IKRPYGMVLVTGPTGSGKTVSLYTFLNMLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIMV
GEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRLCSCKREGDLAPQVLLD
AGFTEADIDGSWKPYHPVGCERCNGSGYKGRCGIYQVMPISEAMQQIILAHGTALQIAEQARKEGVLSLRDAGLQKVKQG
ITSLEEVLATTNN

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=327515 EHF44_RS07830 WP_124683221.1 1332074..1333795(-) (pilF) [Cupriavidus pauculus strain FDAARGOS_614]
ATGACACTAGGTCTTGCGCTTGCCCAGAGCCGGCGGATCGCGCCCGCGCTGCTCGCCCAGCTGGAGCAGTCGGCACGGGA
GAAGAAAACGCAGCTGATCGACGAGATCATCGGCAGCGGCACGATGTCCGCGCACGACGTGGCGATCTTCGCGGCAGACA
AGTACCAGTTGCCGCTGCTGGACCTGTCCCAGTACAACCTCAACCGCGTGCCACCGGCTCTGGCCGGCAACCGGGAATTC
CACGCCCACCGGCTGCTGCCGCTGGGCCGGCGCGAGAACCGGGTGATCGTGGCGATGTCGGACCCGGCCAACCAGGCCGG
CGTCGAGGCCATCCGCCAGAAGCTGAACCTGCCCGTGGAAACGGTCGTGGTGGAGCACGACAAGCTGATGAAGCACGTGC
GGGCGGCCGGCGAGGCGCTGGGCACCATGAAAAACATCTCGCCGGTCACGCCCACCGCGCAGAAGATGATCGAATACGAT
CCGTCGGCCGCGGCTGCCGCCCGCAAGGGGCCGGGCAACGAGATCGACGACGCGCCGGTCGTGCGGTTCCTGCAGAAGCT
GCTGACCGAGGCGTTCAACCGCGGCGCCTCAGACCTGCATTTCGAACCGTTCGAGTTCTTCTACCGCATCCGCTTCCGCG
TGGACGGCGTGCTACAGGAAGTGGCGCGCCCCCCGCTGGACATCCGCGACAAGATCGCCACGCGCATCAAGGTGCTGTCG
CGGCTGGACATCTCGGAAAAGCGCGTGCCGCAGGACGGCCGGATGAAGCTGCTGATTGCCGTGCCGAAGGACAAGGACGG
CAAGGAGACGACCGACAAGGCCATCGACTTCCGCGTCTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGCGGA
TCCTGGATTCGTCGACCGACAAGCTCGACATCGACCAGCTTGGCTACGAGCCCAGGCAGAAGGAACTGCTGATGGAAGTG
ATCAAGCGCCCCTACGGGATGGTGCTGGTCACGGGCCCCACGGGCAGCGGCAAGACCGTGTCGCTCTACACGTTCCTGAA
CATGCTGAACCAGGGCGACATCAATATCTCCACGGCCGAGGACCCGGCCGAAATCCAGTTGCCCGGCATCAACCAGGTGA
ACGTCAACGACAAGGCCGGGCTGACGTTTGCCGCCGCGCTGAAGTCGTTCCTGCGGCAGGATCCGGACATCATCATGGTT
GGCGAAATCCGGGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCCACGCTGCA
CACCAACGACGCCCCGACCACGCTGACCCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCGTCGTCGGTGCTGA
TGATTACCGCCCAGCGGCTGGCGCGCCGGCTCTGCTCGTGCAAGCGCGAGGGCGACCTGGCGCCGCAGGTGCTGCTGGAC
GCGGGCTTTACCGAGGCCGACATCGACGGCTCCTGGAAGCCCTACCACCCGGTGGGCTGCGAGCGCTGCAACGGCAGCGG
CTACAAGGGGCGCTGCGGCATCTACCAGGTCATGCCGATTTCCGAGGCGATGCAGCAGATCATCCTGGCGCACGGCACGG
CGCTGCAGATCGCCGAGCAGGCGCGCAAGGAGGGCGTGCTATCGTTGCGCGACGCCGGGCTGCAGAAGGTCAAGCAGGGC
ATCACGTCGCTGGAGGAAGTGCTGGCGACCACGAACAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(186-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G8GYP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

55.37

94.241

0.522
  pilB Acinetobacter baumannii D1279779

50.967

99.302

0.506
  pilB Acinetobacter baylyi ADP1

50.987

97.208

0.496
  pilB Legionella pneumophila strain ERS1305867

48.644

96.51

0.469
  pilB Vibrio cholerae strain A1552

44.714

100

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

45.221

94.939

0.429
  pilB Vibrio campbellii strain DS40M4

44.567

94.764

0.422
  pilF Thermus thermophilus HB27

39.13

100

0.393

Multiple sequence alignment