Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   SPN23F_RS10625 Genome accession   NC_011900
Coordinates   2010169..2011185 (-) Length   338 a.a.
NCBI ID   WP_078134418.1    Uniprot ID   -
Organism   Streptococcus pneumoniae ATCC 700669     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2005169..2016185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPN23F_RS14145 - 2005247..2005378 (-) 132 WP_000768904.1 hypothetical protein -
  SPN23F_RS10580 (SPN23F20660) - 2005379..2006569 (-) 1191 WP_000167766.1 acetate kinase -
  SPN23F_RS10585 (SPN23F20670) comYH 2006620..2007573 (-) 954 WP_000345133.1 class I SAM-dependent methyltransferase Machinery gene
  SPN23F_RS12710 (SPN23F20680) - 2007634..2008228 (-) 595 Protein_2004 class I SAM-dependent methyltransferase -
  SPN23F_RS10600 (SPN23F20700) comGG/cglG 2008331..2008777 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  SPN23F_RS10605 (SPN23F20710) comGF/cglF 2008755..2009216 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPN23F_RS10610 (SPN23F20720) comGE/cglE 2009179..2009481 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SPN23F_RS10615 (SPN23F20730) comGD/cglD 2009444..2009848 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  SPN23F_RS10620 (SPN23F20740) comGC/cglC 2009841..2010167 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  SPN23F_RS10625 (SPN23F20750) comGB/cglB 2010169..2011185 (-) 1017 WP_078134418.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPN23F_RS10630 (SPN23F20760) comGA/cglA/cilD 2011133..2012074 (-) 942 WP_000249573.1 competence type IV pilus ATPase ComGA Machinery gene
  SPN23F_RS10635 (SPN23F20770) - 2012150..2012515 (-) 366 WP_000286415.1 DUF1033 family protein -
  SPN23F_RS10640 (SPN23F20780) - 2012666..2013724 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  SPN23F_RS10645 (SPN23F20790) nagA 2013887..2015038 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38448.51 Da        Isoelectric Point: 9.6162

>NTDB_id=32716 SPN23F_RS10625 WP_078134418.1 2010169..2011185(-) (comGB/cglB) [Streptococcus pneumoniae ATCC 700669]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVIQMRAGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=32716 SPN23F_RS10625 WP_078134418.1 2010169..2011185(-) (comGB/cglB) [Streptococcus pneumoniae ATCC 700669]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGCGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.675

100

0.947
  comGB/cglB Streptococcus mitis NCTC 12261

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment