Detailed information NTG0326605

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EG365_RS06580
• Genome accession: NZ_CP033866
• Coordinates: 1353601..1354659 (-)
• Length: 352 a.a.
• NCBI ID: WP_004644147.1
• Uniprot ID: A0A7U3Y305
• Organism: Acinetobacter sp. FDAARGOS_494
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1348601..1359659

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EG365_RS06555 (EG365_06560)	aroK	1348876..1349445 (-)	570	WP_000643712.1	shikimate kinase AroK	-
EG365_RS06560 (EG365_06565)	pilQ	1349457..1351622 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
EG365_RS06565 (EG365_06570)	pilP	1351685..1352212 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
EG365_RS06570 (EG365_06575)	pilO	1352223..1352963 (-)	741	WP_000076099.1	type 4a pilus biogenesis protein PilO	Machinery gene
EG365_RS06575 (EG365_06580)	pilN	1352960..1353601 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
EG365_RS06580 (EG365_06585)	pilM	1353601..1354659 (-)	1059	WP_004644147.1	pilus assembly protein PilM	Machinery gene
EG365_RS06585 (EG365_06590)	ponA	1354821..1357376 (+)	2556	WP_000736667.1	penicillin-binding protein PBP1a	-
EG365_RS06590 (EG365_06595)	-	1357394..1358218 (-)	825	WP_002022249.1	putative RNA methyltransferase	-
EG365_RS06595 (EG365_06600)	-	1358221..1358553 (-)	333	WP_000169602.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39062.61 Da
• Isoelectric Point: 4.4618

>NTDB_id=326605 EG365_RS06580 WP_004644147.1 1353601..1354659(-) (pilM) [Acinetobacter sp. FDAARGOS_494]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLTNPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=326605 EG365_RS06580 WP_004644147.1 1353601..1354659(-) (pilM) [Acinetobacter sp. FDAARGOS_494]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTA
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A7U3Y305

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	99.716	100	0.997
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423