Detailed information NTG0325644

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IHMA87_RS28205
• Genome accession: NZ_CP041354
• Coordinates: 5885483..5886547 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain AZPAE15042
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5880483..5891547

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IHMA87_RS28180 (IHMA87_05521)	aroK	5881018..5881536 (-)	519	WP_003148769.1	shikimate kinase AroK	-
IHMA87_RS28185 (IHMA87_05522)	pilQ	5881548..5883692 (-)	2145	WP_003148771.1	type 4a pilus secretin PilQ	Machinery gene
IHMA87_RS28190 (IHMA87_05523)	comL/pilP	5883746..5884270 (-)	525	WP_034082521.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
IHMA87_RS28195 (IHMA87_05524)	pilO	5884267..5884890 (-)	624	WP_003148775.1	type 4a pilus biogenesis protein PilO	Machinery gene
IHMA87_RS28200 (IHMA87_05525)	pilN	5884887..5885483 (-)	597	WP_003148777.1	type 4a pilus biogenesis protein PilN	Machinery gene
IHMA87_RS28205 (IHMA87_05526)	pilM	5885483..5886547 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
IHMA87_RS28210 (IHMA87_05527)	-	5886732..5889203 (+)	2472	WP_012077723.1	penicillin-binding protein 1A	-
IHMA87_RS28215 (IHMA87_05528)	-	5889308..5890576 (-)	1269	WP_034082522.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=325644 IHMA87_RS28205 WP_003110888.1 5885483..5886547(-) (pilM) [Pseudomonas aeruginosa strain AZPAE15042]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=325644 IHMA87_RS28205 WP_003110888.1 5885483..5886547(-) (pilM) [Pseudomonas aeruginosa strain AZPAE15042]
GTGCTAGGGCTCATAAAAAAGAAAGCGAATACCTTACTGGGGATCGACATCAGTTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAACGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTG
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACGATCGAGATGGAGGCCGGGCTTTCCGAGGACGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAGGTCGCCATCGACTTCGAGGTCCAGGGTCTTTCCGCGC
GCAATCCCGAGCGCGTGGACGTGCTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTGCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGTTCCTATGCGCTGCTGAGCAGTCAGTTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTGGTGGACATCGGCGCGACCATGACCACGCTCAGCGTCCTGCACAACGGCC
GCACCATCTATACCCGCGAGCAGTTGTTCGGCGGACGCCAGCTCACCGAGGAGATCCAGCGGCGCTACGGGCTTTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGTGGTCTTCCGGATGACTACGACAGCGAAGTCCTGCGTCCGTTCAAGGACGC
CGTGGTCCAGCAGGTTTCCCGTTCGCTGCAGTTCTTCTTCGCCGCCGGCCAGTTCAATGACGTCGACTACATCGTCCTGG
CGGGCGGCACGGCGTCGATCCAGGACCTCGACCGGCTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAACGCCGGCGCCCTGGCCAGCGACGCGCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466