Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AFE_RS00855 Genome accession   NC_011761
Coordinates   160451..162160 (+) Length   569 a.a.
NCBI ID   WP_012536005.1    Uniprot ID   B7J3S9
Organism   Acidithiobacillus ferrooxidans ATCC 23270     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 155451..167160
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AFE_RS00830 (AFE_0178) xth 156035..156808 (+) 774 WP_012536003.1 exodeoxyribonuclease III -
  AFE_RS00835 (AFE_0179) hemH 156805..157818 (+) 1014 WP_009562604.1 ferrochelatase -
  AFE_RS00840 (AFE_0180) - 157886..158374 (+) 489 WP_009562607.1 peptidylprolyl isomerase -
  AFE_RS00845 (AFE_0181) - 158441..159604 (+) 1164 WP_012536004.1 anhydro-N-acetylmuramic acid kinase -
  AFE_RS00850 (AFE_0182) aroE 159585..160481 (+) 897 WP_009562611.1 shikimate dehydrogenase -
  AFE_RS00855 (AFE_0183) pilB 160451..162160 (+) 1710 WP_012536005.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AFE_RS00860 (AFE_0184) - 162276..163892 (+) 1617 WP_236608970.1 nitrogen regulation protein NR(II) -
  AFE_RS00865 (AFE_0185) - 163885..165240 (+) 1356 WP_041645259.1 sigma-54 dependent transcriptional regulator -
  AFE_RS00870 (AFE_0186) - 165237..166037 (+) 801 WP_012536008.1 A24 family peptidase -
  AFE_RS00875 (AFE_0187) coaE 166038..166652 (+) 615 WP_009562646.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62704.42 Da        Isoelectric Point: 6.3292

>NTDB_id=32317 AFE_RS00855 WP_012536005.1 160451..162160(+) (pilB) [Acidithiobacillus ferrooxidans ATCC 23270]
MATNPEHISLTPVLRALVSNGLSDEARLQSLAADPARGKTPLLFYVVEKGAVPAAVLMAHLSARYNMPMLDLDAAVIDAL
LIRKLDKGLMTRYLVLPLSKHGDTLYLAMADPTDFKAVEDVKFNSGLQVMPVLVEANKLVKAVHAAVNSLQGGIDDVFVD
KPHGEEDPEEFDLAQENDGSVEDAPVVRFVRQLLLDAIQREVSDIHLEPYEKDFRVRYRLDGVLQDALHPPVALRDGVTS
RLKILCRLDISERRLPQDGRLRVRVPPARVIDFRVSFLPTSFGETVVLRLLDPASSRVPIEQLGFLPEQRKAFEDAIHRP
YGMILVTGPTGSGKTTTLYTALNILNTGDCNISTAEDPVEIPVYGINQVNINERIGLTFAAALRSFLRQDPDIIMVGEVR
DLETAETAIKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGSVHLVMAQRLVRKLCPHCKKSLRIPERALIEAGF
APEDLQGWRPLGAVGCEQCNKTGYKGRMGLYQVMPVSDAMREIIMHGGTSIDLARQAAQEGVLTMRQNGLKRIREGVTSL
EEVLRVTNL

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=32317 AFE_RS00855 WP_012536005.1 160451..162160(+) (pilB) [Acidithiobacillus ferrooxidans ATCC 23270]
ATGGCTACAAACCCGGAACACATCTCGTTAACACCAGTATTGCGGGCACTCGTCAGTAACGGTTTAAGCGACGAGGCGCG
CTTGCAAAGCCTTGCCGCCGATCCGGCCCGCGGTAAGACGCCGCTGCTGTTTTATGTGGTTGAGAAGGGCGCCGTCCCGG
CCGCGGTGCTCATGGCGCACCTTTCCGCGCGCTACAACATGCCCATGCTCGATCTCGACGCAGCGGTGATCGATGCGTTG
CTCATCCGCAAGCTCGACAAAGGGCTGATGACCCGCTATCTGGTGCTGCCCTTATCGAAGCATGGGGACACCCTCTACCT
CGCCATGGCCGATCCCACGGACTTCAAGGCGGTGGAGGATGTGAAATTTAATTCCGGTTTGCAGGTGATGCCGGTGCTGG
TCGAGGCCAATAAACTGGTCAAGGCGGTACATGCTGCGGTCAACAGCTTGCAGGGCGGGATCGACGACGTATTCGTGGAT
AAGCCGCACGGTGAGGAAGATCCGGAAGAATTCGACCTCGCCCAGGAGAACGACGGCAGTGTCGAGGATGCGCCGGTGGT
GCGCTTTGTCCGACAACTGCTGCTGGATGCCATTCAGCGCGAGGTGTCCGATATTCATCTGGAGCCCTATGAAAAGGATT
TTCGGGTACGTTACCGCCTCGATGGCGTACTGCAGGACGCGCTGCATCCGCCCGTGGCGTTGCGGGACGGGGTCACCTCG
CGCCTGAAGATTCTTTGCCGTCTGGACATTTCCGAGCGACGTCTGCCTCAGGACGGCCGACTGCGCGTCCGCGTGCCCCC
GGCCCGGGTCATCGATTTTCGCGTCTCCTTTCTGCCCACCAGTTTCGGCGAAACGGTCGTGCTGCGCCTGCTGGATCCCG
CCAGTTCCAGGGTGCCCATCGAGCAACTGGGCTTTTTACCAGAGCAGCGCAAAGCCTTTGAGGACGCCATCCATCGGCCC
TACGGCATGATTCTGGTCACCGGGCCCACGGGTTCCGGCAAGACCACGACCCTCTATACCGCCCTGAACATCCTCAACAC
TGGTGACTGCAACATCAGTACCGCAGAGGACCCCGTGGAAATCCCGGTGTACGGGATCAATCAGGTGAACATCAACGAGC
GCATCGGCCTCACCTTCGCCGCAGCCCTGCGCTCCTTCCTGCGCCAGGACCCGGATATCATCATGGTCGGCGAGGTACGC
GATCTGGAAACCGCCGAAACCGCCATCAAGGCCGCCCAGACGGGCCATCTGGTATTGGCCACCCTGCACACCAATGATGC
TCCACAGAGTCTCACCCGCCTCGAGAACATGGGTATTCCCACCTATAACATCGCCGGCAGCGTACATCTGGTGATGGCGC
AGCGACTCGTCCGCAAGCTCTGTCCGCACTGTAAGAAATCCCTGCGCATACCCGAACGGGCCCTGATCGAGGCGGGCTTT
GCGCCCGAGGATCTGCAGGGTTGGCGCCCTCTGGGTGCCGTGGGCTGCGAACAGTGCAATAAAACCGGATATAAGGGCCG
GATGGGGCTTTATCAGGTGATGCCGGTGAGCGACGCCATGCGCGAAATCATCATGCATGGCGGCACTTCCATCGACTTGG
CGCGGCAGGCGGCACAGGAGGGGGTGCTGACCATGCGGCAGAATGGGCTGAAGCGGATCCGGGAGGGCGTGACCAGTCTG
GAGGAGGTGCTGCGGGTGACCAATCTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7J3S9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

48.348

100

0.489
  pilB Acinetobacter baumannii D1279779

48.596

100

0.487
  pilB Acinetobacter baylyi ADP1

47.544

100

0.476
  pilF Neisseria gonorrhoeae MS11

45.633

98.594

0.45
  pilB Vibrio cholerae strain A1552

45.747

92.97

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

42.152

99.649

0.42
  pilB Vibrio campbellii strain DS40M4

44.487

92.443

0.411
  pilF Thermus thermophilus HB27

39.146

98.77

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.313

92.091

0.362

Multiple sequence alignment