Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EAO82_RS20045 Genome accession   NZ_CP033116
Coordinates   4380365..4381429 (-) Length   354 a.a.
NCBI ID   WP_096346864.1    Uniprot ID   A0AA91U207
Organism   Halopseudomonas pelagia strain Kongs-67     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4375365..4386429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAO82_RS20020 (EAO82_20145) aroK 4375957..4376472 (-) 516 WP_096346861.1 shikimate kinase AroK -
  EAO82_RS20025 (EAO82_20150) pilQ 4376478..4378586 (-) 2109 WP_096346907.1 type IV pilus secretin PilQ Machinery gene
  EAO82_RS20030 (EAO82_20155) - 4378643..4379167 (-) 525 WP_096346908.1 pilus assembly protein PilP -
  EAO82_RS20035 (EAO82_20160) - 4379173..4379793 (-) 621 WP_096346862.1 type 4a pilus biogenesis protein PilO -
  EAO82_RS20040 (EAO82_20165) - 4379790..4380365 (-) 576 WP_096346863.1 PilN domain-containing protein -
  EAO82_RS20045 (EAO82_20170) pilM 4380365..4381429 (-) 1065 WP_096346864.1 pilus assembly protein PilM Machinery gene
  EAO82_RS20050 (EAO82_20175) - 4381615..4384026 (+) 2412 WP_096346865.1 penicillin-binding protein 1A -
  EAO82_RS20055 (EAO82_20180) - 4384259..4385950 (+) 1692 WP_096346866.1 AMP-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38278.79 Da        Isoelectric Point: 4.3697

>NTDB_id=321634 EAO82_RS20045 WP_096346864.1 4380365..4381429(-) (pilM) [Halopseudomonas pelagia strain Kongs-67]
MLGLIKKKANTLLGIDISSTTVKLLELSRSGNRYKVEAYAVEPLPPSAVVEKNIVELEGVGQALEKVLARSRSSLKQAAV
AVAGSAVITKTIEMDADLDPEEMEEQIKLEADQYIPYPLDEVAIDFEVQGPSPRNPDRAEVLLAACRRENVDIREAALAL
AGLGARVVEVEAYAMERACDLVIDQLDGDRDEMTVAVIDIGATMMTLSVLSKGRIIYTREQLFGGKQLTDEIQRRYGLSQ
EEAGLAKKQGGLPDDYESEVLGPFKDAVVQQVSRSLQFFFAGGQYNDVDYILLAGGTASIPGLDHLIQQKIGTTTLVANP
FANMTLSNKVNAVALSNDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=321634 EAO82_RS20045 WP_096346864.1 4380365..4381429(-) (pilM) [Halopseudomonas pelagia strain Kongs-67]
GTGCTAGGGCTCATAAAGAAAAAAGCGAATACGCTACTGGGCATTGATATCAGCTCCACTACGGTGAAGCTGCTCGAACT
CAGTCGCTCAGGTAACCGTTATAAGGTCGAGGCCTATGCGGTTGAACCTCTCCCACCTAGTGCGGTGGTAGAAAAAAACA
TCGTCGAGCTTGAAGGTGTTGGCCAGGCATTGGAGAAAGTCCTTGCCCGGTCGCGCAGCTCTCTCAAGCAAGCCGCCGTT
GCGGTCGCCGGTTCTGCGGTGATCACCAAGACCATTGAAATGGATGCGGATCTCGACCCAGAGGAAATGGAAGAGCAGAT
CAAGCTAGAAGCTGATCAGTACATTCCATATCCTCTGGATGAGGTTGCGATAGACTTCGAGGTCCAGGGGCCGTCACCGC
GCAATCCCGATCGAGCTGAAGTGCTACTGGCCGCCTGTCGCCGTGAGAACGTCGATATTCGCGAGGCAGCGCTAGCGCTT
GCCGGACTGGGCGCACGGGTGGTCGAGGTCGAGGCATACGCGATGGAGCGAGCCTGTGATCTGGTCATCGATCAGCTTGA
TGGTGACCGCGATGAGATGACCGTTGCGGTGATCGATATCGGCGCCACCATGATGACCCTCAGCGTATTGAGCAAGGGTC
GGATCATTTATACCCGCGAGCAATTGTTCGGTGGCAAGCAGCTCACTGACGAGATTCAGCGACGCTACGGTTTGTCGCAG
GAAGAGGCGGGTCTGGCGAAAAAACAGGGTGGTTTGCCAGACGATTACGAGAGTGAAGTGCTGGGGCCTTTCAAGGACGC
AGTGGTTCAGCAGGTATCTCGTTCTCTGCAATTCTTCTTTGCCGGTGGCCAGTATAACGATGTGGATTACATCCTTCTTG
CCGGCGGTACAGCCTCCATACCGGGACTTGATCATCTGATACAGCAAAAGATTGGTACCACCACGCTGGTCGCCAACCCC
TTTGCAAACATGACCCTGTCAAACAAAGTCAATGCAGTGGCGCTTAGTAATGATGCGCCGGCATTGATGATTGCCTGTGG
TCTGGCAATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

45.763

100

0.458

Multiple sequence alignment