Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FEV15_RS07410 Genome accession   NZ_CP040397
Coordinates   1533415..1533855 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain BA22372     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1528415..1538855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEV15_RS07390 (FEV15_07390) coaE 1528905..1529525 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  FEV15_RS24230 - 1529550..1529594 (+) 45 WP_120795372.1 protein YacM -
  FEV15_RS07395 (FEV15_07395) guaC 1529750..1530793 (+) 1044 WP_001217338.1 GMP reductase -
  FEV15_RS07400 (FEV15_07400) hofC 1530828..1532030 (-) 1203 WP_000157236.1 protein transport protein HofC -
  FEV15_RS07405 (FEV15_07405) pilB 1532020..1533405 (-) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  FEV15_RS07410 (FEV15_07410) pilA 1533415..1533855 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  FEV15_RS07415 (FEV15_07415) nadC 1534058..1534951 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FEV15_RS07420 (FEV15_07420) ampD 1535039..1535590 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FEV15_RS07425 (FEV15_07425) ampE 1535587..1536441 (+) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  FEV15_RS07430 (FEV15_07430) aroP 1536484..1537857 (-) 1374 WP_001347572.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=321633 FEV15_RS07410 WP_000360895.1 1533415..1533855(-) (pilA) [Escherichia coli strain BA22372]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=321633 FEV15_RS07410 WP_000360895.1 1533415..1533855(-) (pilA) [Escherichia coli strain BA22372]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment