Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EAO82_RS20025 Genome accession   NZ_CP033116
Coordinates   4376478..4378586 (-) Length   702 a.a.
NCBI ID   WP_096346907.1    Uniprot ID   A0AA91U224
Organism   Halopseudomonas pelagia strain Kongs-67     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4371478..4383586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAO82_RS20010 (EAO82_20135) - 4373263..4374780 (-) 1518 WP_096346859.1 AAA family ATPase -
  EAO82_RS20015 (EAO82_20140) aroB 4374777..4375856 (-) 1080 WP_096346860.1 3-dehydroquinate synthase -
  EAO82_RS20020 (EAO82_20145) aroK 4375957..4376472 (-) 516 WP_096346861.1 shikimate kinase AroK -
  EAO82_RS20025 (EAO82_20150) pilQ 4376478..4378586 (-) 2109 WP_096346907.1 type IV pilus secretin PilQ Machinery gene
  EAO82_RS20030 (EAO82_20155) - 4378643..4379167 (-) 525 WP_096346908.1 pilus assembly protein PilP -
  EAO82_RS20035 (EAO82_20160) - 4379173..4379793 (-) 621 WP_096346862.1 type 4a pilus biogenesis protein PilO -
  EAO82_RS20040 (EAO82_20165) - 4379790..4380365 (-) 576 WP_096346863.1 PilN domain-containing protein -
  EAO82_RS20045 (EAO82_20170) pilM 4380365..4381429 (-) 1065 WP_096346864.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 702 a.a.        Molecular weight: 75496.51 Da        Isoelectric Point: 4.4780

>NTDB_id=321633 EAO82_RS20025 WP_096346907.1 4376478..4378586(-) (pilQ) [Halopseudomonas pelagia strain Kongs-67]
MPLTKKITLAAMMALLSPLALAANLENLDVASLPGERVELKLSFDEPITSAPKGYTIDQPARIAIDLPGVKNELGERTRD
LGLGNARSVTVVEANDRTRLIINLTDLVPYATRVDGNDLYVMVGSDSAPGYAQAAPQPAAASAAQPVPAATTDWRATSEP
AVTSPAAMAGRSITSLDFRRGEQGEGNVIVDLSDPSVKVDVAEQGGRVRLTFPDTQLPDALRVRLDVKDFATPVNFVSAS
TSGGNSVINIEPTGFYEHLVYQADNRLIVSFKPLTQTEVEKRRSEAFSYTGEKLSLNFQDIEVRSVLQLIADFTDLNLVA
SDTVQGSITLRLQNVPWDQALDLVLKTKGLDQRKIGNVLLVAPADEIAARERQELESQQQIAELAPLRRELIQVNYARAA
DIASLFASVTAGDGEQRGSITVDDRTNSIIALETQQKLDELRRIVVQLDIPVRQVMIEARIVEANVGFDKALGVRWGGSI
NAGDRFDVFGRNPGGTGQMPTPSTNSPFVDMGVAGATSGIGIGFITNNTILDLQLSAMESTGNGEIISQPKVVTSDKETA
KILKGSEIPYQEASSSGATTTAFAEAVLSLEVTPQITPDNQVIMEVVVTKDEPDFTNEVNGVPTIRKNEVNAKILVGDGE
TIVIGGVFSSETQTTREKVPFLGDLPVLGKVFRRDVSLENKSELLIFLTPRIINNGAITVAR

Nucleotide


Download         Length: 2109 bp        

>NTDB_id=321633 EAO82_RS20025 WP_096346907.1 4376478..4378586(-) (pilQ) [Halopseudomonas pelagia strain Kongs-67]
ATGCCTTTAACCAAAAAAATTACGCTTGCTGCCATGATGGCCTTGCTTAGCCCCCTGGCCTTGGCCGCCAATCTTGAAAA
CTTGGATGTTGCCTCTCTGCCTGGAGAGCGGGTTGAGTTGAAACTGTCTTTTGACGAGCCAATAACTTCGGCGCCAAAAG
GCTACACCATCGATCAGCCGGCGCGCATTGCCATCGATTTGCCTGGTGTAAAGAACGAGCTCGGTGAGCGTACCCGCGAT
CTAGGGTTGGGGAATGCGCGTAGCGTGACTGTGGTCGAGGCTAACGACCGTACCCGTCTGATCATTAACCTTACCGATCT
GGTGCCTTACGCCACCCGAGTAGATGGCAACGATCTATATGTCATGGTCGGCTCCGACAGTGCCCCAGGCTATGCCCAGG
CCGCACCGCAGCCTGCAGCTGCCAGCGCAGCCCAGCCAGTTCCAGCGGCCACCACTGACTGGCGCGCCACGTCAGAGCCT
GCTGTGACCAGCCCGGCGGCCATGGCAGGACGCTCTATCACCAGCCTGGATTTCCGTCGTGGTGAGCAGGGCGAGGGCAA
CGTCATCGTTGATTTGTCCGATCCTTCGGTAAAGGTTGATGTGGCTGAGCAGGGCGGGCGTGTCAGGCTGACATTCCCAG
ATACTCAGTTACCTGATGCGCTGCGCGTGCGCCTGGATGTGAAGGACTTTGCCACGCCGGTCAATTTCGTTAGCGCCAGC
ACCAGCGGCGGCAACAGCGTGATCAACATTGAACCAACCGGCTTTTATGAGCATCTGGTGTACCAGGCGGATAATCGTTT
GATCGTCAGCTTCAAACCATTGACTCAGACTGAAGTGGAAAAACGCCGTTCCGAAGCTTTTTCTTACACCGGCGAGAAAC
TATCGCTGAATTTCCAGGATATCGAAGTACGTTCGGTGCTGCAGTTGATCGCCGACTTTACCGATTTGAATCTGGTCGCC
AGTGACACGGTTCAGGGCAGCATTACGCTGCGCCTGCAGAACGTGCCTTGGGATCAGGCGCTTGATCTGGTACTGAAAAC
CAAGGGTCTGGACCAGCGCAAAATAGGTAATGTGCTCCTGGTGGCTCCGGCAGACGAGATTGCCGCCAGAGAGCGCCAAG
AGCTGGAGTCCCAGCAGCAAATTGCCGAGCTGGCGCCCTTGCGACGTGAGCTGATCCAGGTCAACTACGCTCGTGCTGCC
GACATCGCCAGCCTGTTCGCTTCAGTGACCGCCGGTGACGGTGAGCAACGCGGCTCGATTACGGTCGATGATCGTACCAA
CAGCATCATCGCACTGGAGACGCAGCAGAAGCTGGACGAGCTGCGTCGTATCGTCGTGCAACTGGATATCCCGGTTCGCC
AAGTAATGATCGAGGCTCGCATCGTTGAAGCCAACGTCGGTTTCGACAAGGCTTTGGGCGTTCGTTGGGGCGGCAGTATC
AATGCCGGTGATCGCTTCGATGTATTCGGGCGTAATCCTGGCGGTACGGGTCAGATGCCTACACCTTCAACTAACTCACC
TTTTGTTGATATGGGTGTAGCGGGCGCGACTTCGGGTATCGGTATCGGTTTTATCACCAACAACACCATTCTTGACCTGC
AACTGTCAGCGATGGAAAGCACCGGTAACGGTGAAATTATCTCCCAGCCGAAAGTGGTTACCTCTGATAAGGAAACTGCC
AAGATCCTCAAGGGTTCGGAAATCCCCTACCAGGAAGCCAGCTCCAGTGGCGCCACTACTACAGCCTTCGCTGAAGCCGT
GCTGTCTTTGGAAGTAACGCCGCAGATTACCCCGGACAATCAGGTGATCATGGAAGTCGTGGTAACCAAGGATGAGCCGG
ACTTTACCAACGAGGTTAACGGCGTGCCGACGATTCGCAAGAATGAGGTCAATGCCAAGATCCTGGTTGGTGATGGTGAA
ACCATCGTGATTGGTGGTGTATTTTCCAGCGAAACTCAAACTACTCGCGAAAAGGTTCCTTTCCTCGGCGACCTGCCGGT
TCTCGGCAAAGTCTTCCGTCGTGACGTCTCTCTGGAAAACAAGTCGGAACTGCTTATCTTCCTGACGCCTCGTATCATCA
ACAATGGAGCTATTACGGTTGCTAGGTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(42-120)

Bacterial type II/III secretion system short domain (Secretin_N)

(391-454)

Secretin and TonB N terminus short domain (STN)

(317-363)

Bacterial type II and III secretion system protein (Secretin)

(537-693)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

68.011

100

0.697
  comQ Acinetobacter baylyi ADP1

40.483

100

0.406
  pilQ Acinetobacter baumannii strain A118

38.019

100

0.405
  pilQ Acinetobacter baumannii D1279779

38.019

100

0.405
  pilQ Legionella pneumophila strain Lp02

38.102

97.578

0.372
  pilQ Legionella pneumophila strain ERS1305867

37.956

97.578

0.37

Multiple sequence alignment