Detailed information NTG0032129

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: LLKF_RS12450
• Genome accession: NC_013656
• Coordinates: 2527407..2528408 (-)
• Length: 333 a.a.
• NCBI ID: WP_003131994.1
• Uniprot ID: Q9CDL3
• Organism: Lactococcus lactis subsp. lactis KF147
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2522407..2533408

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLKF_RS12425 (LLKF_2472)	-	2522710..2523147 (-)	438	WP_003131986.1	low molecular weight protein-tyrosine-phosphatase	-
LLKF_RS13655 (LLKF_2473)	-	2523291..2523455 (-)	165	WP_003131988.1	hypothetical protein	-
LLKF_RS12430 (LLKF_2474)	-	2523560..2524177 (-)	618	WP_012898695.1	AAA family ATPase	-
LLKF_RS12435 (LLKF_2475)	-	2524174..2525358 (-)	1185	WP_012898696.1	ABC transporter permease	-
LLKF_RS12440 (LLKF_2476)	-	2525597..2526166 (-)	570	WP_012898697.1	hypothetical protein	-
LLKF_RS12445 (LLKF_2477)	-	2526386..2527270 (-)	885	WP_012898698.1	XRE/MutR family transcriptional regulator	-
LLKF_RS12450 (LLKF_2478)	ruvB	2527407..2528408 (-)	1002	WP_003131994.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
LLKF_RS12455 (LLKF_2479)	ruvA	2528535..2529128 (-)	594	WP_003131995.1	Holliday junction branch migration protein RuvA	Machinery gene
LLKF_RS12460 (LLKF_2480)	hexB	2529241..2531211 (-)	1971	WP_012898699.1	DNA mismatch repair endonuclease MutL	Machinery gene
LLKF_RS12465 (LLKF_2481)	-	2531340..2532035 (-)	696	WP_012898700.1	hypothetical protein	-

Sequence

Protein

• Length: 333 a.a.
• Molecular weight: 37633.17 Da
• Isoelectric Point: 4.5825

>NTDB_id=32129 LLKF_RS12450 WP_003131994.1 2527407..2528408(-) (ruvB) [Lactococcus lactis subsp. lactis KF147]
MNDILNKEPMEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNI
KQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTR
AGMLSNPLRARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVD
KAITDKALSILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQR
AYEHLGYVYNEED

Nucleotide

• Length: 1002 bp

>NTDB_id=32129 LLKF_RS12450 WP_003131994.1 2527407..2528408(-) (ruvB) [Lactococcus lactis subsp. lactis KF147]
ATGAACGATATATTAAACAAAGAGCCAATGGAAGAAGATTACGGTATTGAAAAATCTCTACGTCCGCAATTTTTTAATCA
ATATATTGGTCAAGATAAAGTCAAAGAACAATTAGAAATCTTTATTAAAGCAGCAAAAATGCGTGAAGAAGTTTTGGACC
ATGTATTACTTTTTGGTCCTCCGGGACTTGGTAAGACAACAATGGCATTTGTCATTGCCAATGAGTTGGGAGTTAATATT
AAACAAACAGCGGGTCCTGCCATTGAAAAGCCAGGAGATTTAGTTGCGATTTTGAATGAATTGGAACCTGGCGATGTGCT
ATTTATTGATGAAATTCATCGGATGCCAATGCAAGTTGAAGAAGTTCTTTATAGCGCTATGGAAGATTTCTACATTGATA
TTATGCTTGGTTCTGGTGATGGTTCACGCTCGGTTCATTTAGATTTGCCGCCATTTACTCTAGTTGGGGCAACAACAAGA
GCTGGAATGCTTTCGAATCCATTGCGTGCCCGTTTTGGAATTTCAAGTCATATGGAGTATTATCAAGAGCGTGATTTGGA
AGAAATTGTTAAAAGAACAGCTGATATTTTTGAAGTCGAAGTTATTGATAATGCCGCTTTAGAGATTGCTTTACGTAGTC
GAGGGACTCCGCGGATTGCCAATCGTTTACTTAAACGAGTGCGTGATTTTGCTCAAATAATGGGGGATGGCCGAGTGGAT
AAAGCAATTACCGATAAGGCCTTGTCTATTCTTGATGTTGATGCCGCTGGTTTGGACTATATTGACCAAAAAATTCTCCG
CACAATGATTGAAATGTACCACGGTGGTCCTGTTGGGATTGGAACTTTGGCGGTAAATATTGCCGAAGACCGTGAAACGG
TCGAGGATATGTATGAACCTTATTTGATTCAAAAAGGATTTTTGATGAGAACTAAACAGGGACGTAAGGTGACCCAAAGA
GCCTATGAACACCTAGGTTATGTTTACAATGAAGAAGATTGA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: Q9CDL3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	78.614	99.7	0.784
ruvB	Streptococcus pneumoniae D39	78.614	99.7	0.784
ruvB	Streptococcus pneumoniae TIGR4	78.313	99.7	0.781
ruvB	Bacillus subtilis subsp. subtilis str. 168	61.491	96.697	0.595
ruvB	Helicobacter pylori 26695	51.242	96.697	0.495
ruvB	Synechocystis sp. PCC 6803	51.613	93.093	0.48