Detailed information NTG0320951

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: D9777_RS15035
• Genome accession: NZ_CP033054
• Coordinates: 2985186..2985800 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain Bac57
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2980186..2990800

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9777_RS15005	rpsT	2980489..2980755 (+)	267	WP_021494335.1	30S ribosomal protein S20	-
D9777_RS15010	holA	2980772..2981813 (-)	1042	Protein_2889	DNA polymerase III subunit delta	-
D9777_RS15015	-	2981853..2982004 (-)	152	Protein_2890	hypothetical protein	-
D9777_RS15020	-	2982045..2982179 (+)	135	WP_003152870.1	YqzM family protein	-
D9777_RS15025	comEC	2982198..2984549 (-)	2352	WP_021494336.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
D9777_RS15030	-	2984550..2985119 (-)	570	WP_003152868.1	ComE operon protein 2	-
D9777_RS15035	comEA	2985186..2985800 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
D9777_RS15040	comER	2985859..2986680 (+)	822	WP_014418420.1	late competence protein ComER	-
D9777_RS15045	-	2986749..2987486 (-)	738	WP_021494337.1	class I SAM-dependent methyltransferase	-
D9777_RS15050	rsfS	2987483..2987839 (-)	357	WP_007408260.1	ribosome silencing factor	-
D9777_RS15055	yqeK	2987857..2988417 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
D9777_RS15060	-	2988407..2988976 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
D9777_RS15065	yhbY	2988987..2989277 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
D9777_RS15070	aroE	2989271..2990113 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=320951 D9777_RS15035 WP_014418419.1 2985186..2985800(-) (comEA) [Bacillus velezensis strain Bac57]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=320951 D9777_RS15035 WP_014418419.1 2985186..2985800(-) (comEA) [Bacillus velezensis strain Bac57]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363