Detailed information NTG0319946

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: D9C08_RS17215
• Genome accession: NZ_CP032872
• Coordinates: 3205135..3205752 (-)
• Length: 205 a.a.
• NCBI ID: WP_046160608.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain 2KL1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3200135..3210752

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9C08_RS17185 (D9C08_17185)	rpsT	3200437..3200703 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
D9C08_RS17190 (D9C08_17190)	holA	3200718..3201761 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
D9C08_RS17195 (D9C08_17195)	-	3201801..3201950 (-)	150	WP_003229985.1	hypothetical protein	-
D9C08_RS17200 (D9C08_17200)	yqzM	3201991..3202125 (+)	135	WP_003229983.1	YqzM family protein	-
D9C08_RS17205 (D9C08_17205)	comEC	3202165..3204495 (-)	2331	WP_121509424.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
D9C08_RS17210 (D9C08_17210)	comEB	3204499..3205068 (-)	570	WP_003229978.1	ComE operon protein 2	-
D9C08_RS17215 (D9C08_17215)	comEA	3205135..3205752 (-)	618	WP_046160608.1	competence protein ComEA	Machinery gene
D9C08_RS17220 (D9C08_17220)	comER	3205836..3206657 (+)	822	WP_032726195.1	late competence protein ComER	-
D9C08_RS17225 (D9C08_17225)	yqeM	3206723..3207466 (-)	744	WP_069837641.1	class I SAM-dependent methyltransferase	-
D9C08_RS17230 (D9C08_17230)	rsfS	3207463..3207819 (-)	357	WP_014480315.1	ribosome silencing factor	-
D9C08_RS17235 (D9C08_17235)	yqeK	3207837..3208397 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
D9C08_RS17240 (D9C08_17240)	nadD	3208387..3208956 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
D9C08_RS17245 (D9C08_17245)	yhbY	3208968..3209258 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
D9C08_RS17250 (D9C08_17250)	aroE	3209252..3210094 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21738.48 Da
• Isoelectric Point: 4.7284

>NTDB_id=319946 D9C08_RS17215 WP_046160608.1 3205135..3205752(-) (comEA) [Bacillus subtilis subsp. subtilis strain 2KL1]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=319946 D9C08_RS17215 WP_046160608.1 3205135..3205752(-) (comEA) [Bacillus subtilis subsp. subtilis strain 2KL1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376