Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D7H65_RS23930 Genome accession   NZ_CP032569
Coordinates   5041157..5042860 (+) Length   567 a.a.
NCBI ID   WP_140786471.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain BA7823     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5036157..5047860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7H65_RS23910 (D7H65_23920) nadC 5038615..5039463 (+) 849 WP_033990663.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D7H65_RS23920 (D7H65_23930) - 5039627..5040184 (-) 558 WP_224754157.1 hypothetical protein -
  D7H65_RS23925 (D7H65_23935) - 5040389..5040928 (-) 540 WP_033938465.1 pilin -
  D7H65_RS23930 (D7H65_23940) pilB 5041157..5042860 (+) 1704 WP_140786471.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D7H65_RS23935 (D7H65_23945) pilC 5042864..5044081 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  D7H65_RS23940 (D7H65_23950) pilD 5044084..5044956 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  D7H65_RS23945 (D7H65_23955) coaE 5044953..5045564 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  D7H65_RS23950 (D7H65_23960) yacG 5045561..5045761 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  D7H65_RS23955 (D7H65_23965) - 5045798..5046007 (-) 210 WP_003094660.1 hypothetical protein -
  D7H65_RS23960 (D7H65_23970) - 5046113..5046802 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  D7H65_RS23965 (D7H65_23975) - 5046799..5047269 (-) 471 WP_003094664.1 hypothetical protein -
  D7H65_RS23970 (D7H65_23980) - 5047266..5047691 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62594.49 Da        Isoelectric Point: 5.5603

>NTDB_id=316970 D7H65_RS23930 WP_140786471.1 5041157..5042860(+) (pilB) [Pseudomonas aeruginosa strain BA7823]
MNDTIQLSGLARQPVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=316970 D7H65_RS23930 WP_140786471.1 5041157..5042860(+) (pilB) [Pseudomonas aeruginosa strain BA7823]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCCCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAAAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGCACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACTTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGATCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.58
  pilB Acinetobacter baylyi ADP1

57.092

99.471

0.568
  pilB Legionella pneumophila strain ERS1305867

54.93

100

0.55
  pilF Neisseria gonorrhoeae MS11

50.266

99.295

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

51.948

95.062

0.494
  pilB Vibrio cholerae strain A1552

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.584

99.647

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.474

0.376

Multiple sequence alignment