Detailed information NTG0316918

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: PA34_RS26105
• Genome accession: NZ_CP032552
• Coordinates: 5442446..5444149 (+)
• Length: 567 a.a.
• NCBI ID: WP_003141354.1
• Uniprot ID: A0A0H2ZH73
• Organism: Pseudomonas aeruginosa strain PA34
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5431211..5441615	5442446..5444149	flank	831

Gene organization within MGE regions

Location: 5431211..5444149

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA34_RS26035 (PA34_026035)	-	5431211..5432032 (-)	822	WP_062832572.1	hypothetical protein	-
PA34_RS33315	-	5432347..5432472 (+)	126	WP_265324936.1	hypothetical protein	-
PA34_RS32925	-	5432601..5432762 (+)	162	WP_162998314.1	hypothetical protein	-
PA34_RS26040 (PA34_026040)	-	5433174..5433488 (+)	315	WP_062832573.1	helix-turn-helix domain-containing protein	-
PA34_RS26045 (PA34_026045)	-	5433492..5435891 (+)	2400	WP_079767670.1	bifunctional DNA primase/polymerase	-
PA34_RS26050 (PA34_026050)	-	5436482..5436997 (+)	516	WP_062832575.1	hypothetical protein	-
PA34_RS26055 (PA34_026055)	-	5436987..5437235 (+)	249	WP_062832576.1	hypothetical protein	-
PA34_RS26060 (PA34_026060)	-	5437210..5437656 (+)	447	WP_062832577.1	hypothetical protein	-
PA34_RS26065 (PA34_026065)	-	5437882..5438598 (+)	717	WP_062832578.1	hypothetical protein	-
PA34_RS26070 (PA34_026070)	-	5438591..5438866 (+)	276	WP_050714322.1	hypothetical protein	-
PA34_RS26075 (PA34_026075)	-	5439084..5439551 (+)	468	WP_062832579.1	hypothetical protein	-
PA34_RS26080 (PA34_026080)	-	5439545..5439925 (+)	381	WP_062832580.1	hypothetical protein	-
PA34_RS26085 (PA34_026085)	-	5440016..5440483 (-)	468	WP_062832581.1	hypothetical protein	-
PA34_RS26095 (PA34_026095)	-	5440917..5441615 (-)	699	WP_016254081.1	hypothetical protein	-
PA34_RS26100 (PA34_026100)	-	5441678..5442217 (-)	540	WP_003141352.1	pilin	-
PA34_RS26105 (PA34_026105)	pilB	5442446..5444149 (+)	1704	WP_003141354.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 567 a.a.
• Molecular weight: 62610.54 Da
• Isoelectric Point: 5.5603

>NTDB_id=316918 PA34_RS26105 WP_003141354.1 5442446..5444149(+) (pilB) [Pseudomonas aeruginosa strain PA34]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFP
EDKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide

• Length: 1704 bp

>NTDB_id=316918 PA34_RS26105 WP_003141354.1 5442446..5444149(+) (pilB) [Pseudomonas aeruginosa strain PA34]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTAATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCAGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGCACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCAGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCTA
GCGCGCAGATGGGCATCGACGCCCTGGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAATCTGATCATCGCCCAGC
GCCTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCG
GAAGACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

3D structure

• AlphaFold DB: A0A0H2ZH73

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.719	100	0.58
pilB	Acinetobacter baylyi ADP1	57.27	99.471	0.57
pilB	Legionella pneumophila strain ERS1305867	55.106	100	0.552
pilF	Neisseria gonorrhoeae MS11	50.444	99.295	0.501
pilB	Vibrio parahaemolyticus RIMD 2210633	51.948	95.062	0.494
pilB	Vibrio cholerae strain A1552	50.182	97.002	0.487
pilB	Vibrio campbellii strain DS40M4	50.832	95.414	0.485
pilF	Thermus thermophilus HB27	38.584	99.647	0.384
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.189	93.474	0.376