Detailed information NTG0315715

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: D3880_RS20505
• Genome accession: NZ_CP032419
• Coordinates: 4516243..4517307 (-)
• Length: 354 a.a.
• NCBI ID: WP_119895262.1
• Uniprot ID: A0A385Z7V2
• Organism: Pseudomonas cavernae strain K2W31S-8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4511243..4522307

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D3880_RS20480 (D3880_20485)	aroK	4511886..4512404 (-)	519	WP_119895257.1	shikimate kinase AroK	-
D3880_RS20485 (D3880_20490)	pilQ	4512409..4514472 (-)	2064	WP_119895258.1	type IV pilus secretin PilQ	Machinery gene
D3880_RS20490 (D3880_20495)	pilP	4514527..4515021 (-)	495	WP_119895259.1	type 4a pilus biogenesis lipoprotein PilP	-
D3880_RS20495 (D3880_20500)	pilO	4515051..4515674 (-)	624	WP_119895260.1	type 4a pilus biogenesis protein PilO	-
D3880_RS20500 (D3880_20505)	-	4515671..4516243 (-)	573	WP_119895261.1	PilN domain-containing protein	-
D3880_RS20505 (D3880_20510)	pilM	4516243..4517307 (-)	1065	WP_119895262.1	pilus assembly protein PilM	Machinery gene
D3880_RS20510 (D3880_20515)	-	4517492..4519927 (+)	2436	WP_162935026.1	penicillin-binding protein 1A	-
D3880_RS20515 (D3880_20520)	-	4519994..4521262 (-)	1269	WP_119895264.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38023.40 Da
• Isoelectric Point: 4.4526

>NTDB_id=315715 D3880_RS20505 WP_119895262.1 4516243..4517307(-) (pilM) [Pseudomonas cavernae strain K2W31S-8]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALTRVLVKAKTGVKTAVV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGPSARNPERVDVLLAACRKENVEVREAALAL
SGMTAKVVDVEAYALERAYGLLAPQFGNDHEELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTDEIQRRYGLSV
EEAGLAKKQGGLPDDYESEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=315715 D3880_RS20505 WP_119895262.1 4516243..4517307(-) (pilM) [Pseudomonas cavernae strain K2W31S-8]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATACGCTTCTGGGGATAGATATCAGTTCGACCTCAGTCAAGCTCTTGGAATT
GAGTCGCTCAGGAAGCCGCTACAAGGTAGAGGCATACGCCGTTGAGCCCCTTCCCCCAAATGCAGTGGTCGAGAAGAACA
TCGCCGAGCTGGAGGGGGTCGGGCAGGCCTTGACCCGCGTCCTGGTCAAGGCCAAGACCGGTGTGAAAACCGCCGTGGTG
GCGGTGGCGGGTTCTGCTGTGATCACCAAAACCATCGAGATGGATGCGGGGCTTTCCGATGATGAGCTGGAGAATCAGCT
GAAAATCGAAGCTGACCAGTACATTCCTTATCCGCTTGAAGAAGTCGCCATCGATTTCGAGGTTCAGGGCCCTTCGGCCC
GCAACCCTGAGCGGGTGGATGTGTTGCTTGCCGCCTGCCGCAAGGAGAATGTCGAGGTGCGTGAGGCGGCCCTGGCCTTG
TCGGGCATGACGGCTAAGGTCGTCGATGTCGAGGCCTATGCTCTCGAACGGGCTTACGGCCTGCTGGCTCCGCAATTCGG
TAATGATCACGAAGAATTGACGGTGGCAGTCGTGGACATTGGTGCCACCATGACCACTCTGAGCGTTCTGCATAACGGCC
GCACCATCTATACCCGCGAGCAGCTGTTTGGTGGTAAGCAGCTGACTGACGAGATTCAGCGTCGTTATGGCTTGTCAGTC
GAAGAAGCGGGCCTTGCTAAGAAGCAAGGCGGTCTGCCGGACGACTATGAGAGTGAGGTTCTGCAGCCATTCAAGGACGC
TGTGGTGCAGCAGGTTTCACGCTCGTTGCAGTTTTTCTTCGCGGCTGGCCAATTCAATGATGTGGATTACATCCTCCTGG
CGGGCGGAACTGCCTCCATTCCCGATCTGGATCGGCTGATCCAGCAGAAGATCGGTACCCAGACGCTGGTGGCCAATCCG
TTTGCCGATATGGCGCTTAGCAGCAAGGTCAATGCTGGGGCTTTGGCTAGCGATGCTCCGGCCTTGATGATTGCGTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A385Z7V2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.863	100	0.573
comM	Acinetobacter nosocomialis M2	56.863	100	0.573
comM	Acinetobacter baylyi ADP1	53.221	100	0.537
pilM	Legionella pneumophila strain ERS1305867	45.763	100	0.458