Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   D3880_RS20485 Genome accession   NZ_CP032419
Coordinates   4512409..4514472 (-) Length   687 a.a.
NCBI ID   WP_119895258.1    Uniprot ID   A0A385Z628
Organism   Pseudomonas cavernae strain K2W31S-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4507409..4519472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3880_RS20470 (D3880_20475) - 4509091..4510719 (-) 1629 WP_119895255.1 AAA family ATPase -
  D3880_RS20475 (D3880_20480) aroB 4510737..4511834 (-) 1098 WP_119895256.1 3-dehydroquinate synthase -
  D3880_RS20480 (D3880_20485) aroK 4511886..4512404 (-) 519 WP_119895257.1 shikimate kinase AroK -
  D3880_RS20485 (D3880_20490) pilQ 4512409..4514472 (-) 2064 WP_119895258.1 type IV pilus secretin PilQ Machinery gene
  D3880_RS20490 (D3880_20495) pilP 4514527..4515021 (-) 495 WP_119895259.1 type 4a pilus biogenesis lipoprotein PilP -
  D3880_RS20495 (D3880_20500) pilO 4515051..4515674 (-) 624 WP_119895260.1 type 4a pilus biogenesis protein PilO -
  D3880_RS20500 (D3880_20505) - 4515671..4516243 (-) 573 WP_119895261.1 PilN domain-containing protein -
  D3880_RS20505 (D3880_20510) pilM 4516243..4517307 (-) 1065 WP_119895262.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 687 a.a.        Molecular weight: 74018.03 Da        Isoelectric Point: 4.8032

>NTDB_id=315714 D3880_RS20485 WP_119895258.1 4512409..4514472(-) (pilQ) [Pseudomonas cavernae strain K2W31S-8]
MKSSLSRLCVSFLAALLSPALLAANLQALDVAALPGDRVELKLAFDEPVAAPRGYTIEQPARIALDLPGVKNGLGTKNRE
LGVGNARSVTVVEAKDRTRLIINLTTLAPYSTRTEGNALYVVVGESSNAAPVSRVAPSAPIVAKKSYAPSGRVIRNIDFQ
RGEQGEGNVVIELSDPSVSPDIQEQGGKIRLDFAKTQLPESLRVRLDVKDFATPVQFVSAAGGADKASITIEPTGFYDYL
AYQADNKLTVSIKPLNQQDVEKRKAESFAYNGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADIAKLFQSVTSGDASKDER
GSITVDDRTNSIIAYQTQDKLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVEWRGVSVGDNNFVVGGSNSLVKN
SDPLEIDAGTFVDLGATGASSGLNLGFITDNTILDLELSAMEKTGNGEVVSQPKVVTSDKETAKILKGSEVPYQEASSSG
ATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFSVVASTGGVPAINKNEVNAKVLVADGETIVIGGVFSNTQTTS
VDKVPFLGDLPFLGRLFRRDVVQNKKSELLVFLTPRIMNNQAIAVSQ

Nucleotide


Download         Length: 2064 bp        

>NTDB_id=315714 D3880_RS20485 WP_119895258.1 4512409..4514472(-) (pilQ) [Pseudomonas cavernae strain K2W31S-8]
ATGAAGAGCTCTCTTTCGCGCCTCTGCGTTTCTTTTCTTGCGGCGCTTCTGTCGCCGGCTTTGCTGGCGGCAAATCTGCA
GGCACTTGATGTGGCTGCTTTGCCGGGGGATCGGGTGGAGCTCAAACTGGCGTTTGATGAGCCAGTGGCTGCGCCACGTG
GTTATACCATCGAACAGCCGGCGCGCATCGCGCTGGATCTTCCGGGGGTGAAGAACGGCCTTGGCACAAAGAATCGCGAG
CTGGGCGTTGGTAATGCTCGCAGCGTTACGGTGGTGGAGGCCAAGGATCGTACCCGGCTGATCATCAATCTCACCACCCT
GGCCCCCTATAGCACGCGCACCGAAGGTAATGCCCTTTATGTGGTGGTTGGTGAGAGCTCCAACGCTGCGCCGGTATCGC
GCGTGGCGCCGTCGGCACCCATAGTTGCCAAGAAAAGCTATGCACCTTCAGGGCGGGTTATCCGCAATATTGACTTTCAG
CGGGGCGAGCAGGGTGAGGGCAATGTAGTGATCGAGCTGTCCGATCCTTCGGTAAGCCCGGATATTCAAGAGCAAGGTGG
CAAGATCCGCCTGGATTTCGCCAAAACGCAATTGCCTGAAAGCCTCCGGGTCCGTCTGGATGTCAAGGATTTTGCGACGC
CTGTGCAGTTCGTCAGTGCTGCGGGGGGGGCGGATAAAGCCAGTATCACCATTGAGCCAACGGGCTTCTATGACTACTTG
GCCTATCAAGCTGACAACAAGTTGACCGTCAGCATCAAGCCGCTGAATCAGCAGGATGTTGAGAAACGCAAGGCCGAGAG
CTTCGCCTACAACGGCGAGAAGCTTTCGTTGAATTTCCAGGATATCGATGTGCGTTCGGTGCTGCAGTTGATCGCCGACT
TCACTGATCTGAACTTGGTGGCCAGTGATACCGTACAGGGCAATATCACTTTGCGTCTGCAAAATGTGCCGTGGGACCAG
GCGCTTGATCTGGTGTTGAAAACCAAGGGGTTGGACAAGCGCAAGGTGGGCAACGTTCTGCTGGTTGCTCCGGCTGATGA
AATTGCTGCGCGTGAGCGCCAAGAGCTGGAGTCGCAGAAGCAAATTGCCGAGCTTGCGCCGCTGCGCCGCGAGCTGATTC
AAGTCAACTATGCCAAGGCGGCCGATATCGCCAAGCTGTTCCAGTCGGTCACTAGTGGCGATGCCAGTAAAGATGAGCGT
GGTTCCATTACGGTGGATGATCGCACCAACAGCATTATCGCCTACCAGACTCAGGATAAGCTCGATGAGCTGCGGCGCAT
CGTGTCGCAATTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAATGTCGATTACGACAAGT
CGCTCGGGGTTGAGTGGCGTGGCGTGAGTGTTGGCGACAATAACTTTGTGGTCGGCGGCTCGAACAGTCTAGTCAAGAAC
AGTGACCCTTTGGAAATCGATGCTGGGACCTTTGTCGATCTAGGTGCCACCGGGGCAAGCTCCGGATTGAATCTGGGATT
TATCACCGACAACACCATTCTCGATCTGGAACTGTCGGCCATGGAAAAGACCGGCAACGGCGAGGTCGTGTCGCAACCGA
AGGTGGTCACATCGGATAAGGAAACTGCCAAAATCCTGAAAGGTTCCGAAGTGCCCTACCAAGAAGCCAGTTCCAGCGGT
GCAACCAGTACTTCCTTTAAAGAGGCGGCGCTGTCGCTAGAGGTTACGCCGCAAATCACGCCGGATAACCGAATCATCAT
GGAGGTCAAGGTTACCAAGGATGCCCCGGACTTTTCGGTCGTGGCGTCTACGGGGGGGGTTCCCGCGATCAACAAAAATG
AGGTCAATGCCAAGGTGTTGGTGGCCGACGGAGAGACTATCGTGATCGGCGGGGTGTTTTCCAACACCCAGACCACGTCC
GTCGACAAGGTGCCGTTTCTCGGCGACCTGCCCTTCCTTGGTCGACTGTTTAGGCGCGATGTTGTGCAGAATAAGAAGTC
CGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAACAATCAGGCCATTGCTGTGAGCCAGTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(520-678)

AMIN domain (AMIN)

(38-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A385Z628

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

76.536

100

0.798
  comQ Acinetobacter baylyi ADP1

43.516

100

0.44
  pilQ Acinetobacter baumannii D1279779

41.774

100

0.425
  pilQ Acinetobacter baumannii strain A118

41.774

100

0.425
  pilQ Legionella pneumophila strain ERS1305867

39.013

97.38

0.38
  pilQ Legionella pneumophila strain Lp02

39.013

97.38

0.38

Multiple sequence alignment